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Phosphopeptides interacting with colloidal calcium phosphate isolated by tryptic hydrolysis of bovine casein micelles

Published online by Cambridge University Press:  01 June 2009

Valerie Gagnaire ,
Alice Pierre ,
Daniel Molle  and
Joelle Leonil
Valerie Gagnaire
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherche de Technologic Laitière, 65 rue de St-Brieuc, F-35042 Rennes Cedex, France
Alice Pierre
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherche de Technologic Laitière, 65 rue de St-Brieuc, F-35042 Rennes Cedex, France
Daniel Molle
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherche de Technologic Laitière, 65 rue de St-Brieuc, F-35042 Rennes Cedex, France
Joelle Leonil
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherche de Technologic Laitière, 65 rue de St-Brieuc, F-35042 Rennes Cedex, France
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Summary

After extended tryptic hydrolysis of large bovine casein micelles, a mineral-rich peptide fraction was recovered by ultracentrifugation. Its mineral part contained 72% of the colloidal Ca and 49% of the colloidal Pi originally present in the native micelle. Colloidal nitrogenous components were also present, amounting to 27% of the original N content. They contained most of the phosphopeptides and 82% of the micellar phosphoseryl residues. These tryptic peptides were characterized by reversed-phase HPLC on-line electrospray ion source–mass spectrometry analysis. Among the peptides produced 14 phosphopeptides were identified: αs2-CN(l–24), αs2-CN(1–21), αs1-CN(43–79), αs1-CN(35–79)7P, αs1-CN(35–79)8P, αs1-CN(37–79), αs1-CN(104–119), αs1-CN(104–124), β-CN(1–25), β-CN(1–28), β-CN(1–29), β-CN(30–97), β-CN(33–97) and β-CN(29–97). The proportion of the phosphopeptides interacting with colloidal calcium phosphate was correlated with their relative content of phosphoserine residues, since phosphopeptides containing more than four phos-phoserine residues were consistently present within this fraction. It also appeared that other types of peptides, some of them hydrophobic in nature, were also partly or completely present within the colloidal fraction, including αs1-CN(91–100), αs1-CN(152–193), αs1-CN(23–34), αs1-CN(125–193), αs1-CN(125–199), β-CN(177–209), β-CN( 184–209), β-CN(114–169) and β-CN(108–169). Their possible involvement in the micellar backbone is discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 3 , August 1996 , pp. 405 - 422
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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References

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