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Phagocytic activity of bovine polymorphonuclear neutrophil leucocytes

Published online by Cambridge University Press:  01 June 2009

Alfonso Zecconi ,
Valerio Bronzo ,
Renata Piccinini ,
Giovanna Spreafico  and
Gianfranco Ruffo
Alfonso Zecconi
Affiliation:
Università degli Studi di Milano, Istituto Malattie Infettive Profilassi e Polizia Veterinaria, CNR Centro Studio Patologia della Mammella, Via Celoria 10, 20133 Milano, Italia
Valerio Bronzo
Affiliation:
Università degli Studi di Milano, Istituto Malattie Infettive Profilassi e Polizia Veterinaria, CNR Centro Studio Patologia della Mammella, Via Celoria 10, 20133 Milano, Italia
Renata Piccinini
Affiliation:
Università degli Studi di Milano, Istituto Malattie Infettive Profilassi e Polizia Veterinaria, CNR Centro Studio Patologia della Mammella, Via Celoria 10, 20133 Milano, Italia
Giovanna Spreafico
Affiliation:
Università degli Studi di Milano, Istituto Malattie Infettive Profilassi e Polizia Veterinaria, CNR Centro Studio Patologia della Mammella, Via Celoria 10, 20133 Milano, Italia
Gianfranco Ruffo
Affiliation:
Università degli Studi di Milano, Istituto Malattie Infettive Profilassi e Polizia Veterinaria, CNR Centro Studio Patologia della Mammella, Via Celoria 10, 20133 Milano, Italia
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Summary

Two different investigations were conducted on the chemiluminescent activity of bovine milk polymorphonuclear neutrophil leucocytes (PMN) activated by different stimuli: zymosan, derived from the wall of Saccharomyces cerevisiae (Experiment A), and Streptococcus uberis and Escherichia coli (Experiment B). In Experiment A, a quarter with a phagocytic activity of PMN < 20 mV/1000 PMN following stimulation with zymosan was found to be 23 times more likely to be a clinical mastitis case than a quarter with phagocytic activity above this threshold value. In Experiment B, calculation of the odds ratio showed similar results following stimulation with Str. uberis or Esch. coli. These results provide evidence that immunocompromisation of mammary gland defences could predispose to clinical mastitis. They also support the need to challenge phagocytic cells with appropriate stimuli, and the Esch. coli test seems to be the most sensitive.

Type
Original Articles
Information
Journal of Dairy Research , Volume 61 , Issue 2 , May 1994 , pp. 271 - 279
Copyright
Copyright © Proprietors of Journal of Dairy Research 1994

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