Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-26T13:04:36.137Z Has data issue: false hasContentIssue false

Pategrás cheese as a suitable carrier for six probiotic cultures

Published online by Cambridge University Press:  25 February 2010

Carina Bergamini*
Affiliation:
Instituto de Lactología Industrial, Universidad Nacional del Litoral – Consejo Nacional de Investigaciones Científicas y Técnicas. 1° de mayo 3250, S3000AOMSanta Fe, Argentina
Erica Hynes
Affiliation:
Instituto de Lactología Industrial, Universidad Nacional del Litoral – Consejo Nacional de Investigaciones Científicas y Técnicas. 1° de mayo 3250, S3000AOMSanta Fe, Argentina
Carlos Meinardi
Affiliation:
Instituto de Lactología Industrial, Universidad Nacional del Litoral – Consejo Nacional de Investigaciones Científicas y Técnicas. 1° de mayo 3250, S3000AOMSanta Fe, Argentina
Viviana Suárez
Affiliation:
Instituto de Lactología Industrial, Universidad Nacional del Litoral – Consejo Nacional de Investigaciones Científicas y Técnicas. 1° de mayo 3250, S3000AOMSanta Fe, Argentina
Andrea Quiberoni
Affiliation:
Instituto de Lactología Industrial, Universidad Nacional del Litoral – Consejo Nacional de Investigaciones Científicas y Técnicas. 1° de mayo 3250, S3000AOMSanta Fe, Argentina
Carlos Zalazar
Affiliation:
Instituto de Lactología Industrial, Universidad Nacional del Litoral – Consejo Nacional de Investigaciones Científicas y Técnicas. 1° de mayo 3250, S3000AOMSanta Fe, Argentina
*
*For correspondence; e-mail: [email protected]

Abstract

The viability of five single-strain and one three-strain probiotic cultures was assessed during Pategrás cheese ripening. Probiotics were inoculated into cheese-milk after a pre-incubation step – intended to improve their survival – or directly as a lyophilised culture; control cheeses without probiotics were also obtained. pH of probiotic and control cheeses was similar, except in probiotic cheeses containing the strain Lb. acidophilus B or the mixed culture. In these cases, the probiotic cheeses were more acid than their respective control cheeses. All the probiotics tested maintained counts above 107 cfu/g during the shelf-life settled for the product. Strains of the Lb. casei group: Lb. paracasei, Lb. casei and Lb. rhamnosus reached and kept the highest cell concentration during cheese ripening, followed by Lb. acidophilus and bifidobacteria. The direct addition of the probiotic cultures was more efficient than their inoculation after a pre-incubation step, for all the probiotics assayed. We have provided evidence that support the use of Pategrás cheese as a performing food-based vehicle for probiotic bacteria.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bergamini, C, Hynes, E, Quiberoni, A, Suárez, V & Zalazar, C 2005 Probiotic bacteria as adjunct starters: influence of the addition methodology on their survival in a semi-hard Argentinean cheese. Food Research International 38 597604CrossRefGoogle Scholar
Bertazzoni Minelli, E, Benini, A, Marzotto, M, Sbarbati, A, Ruzzenente, O, Ferrario, R, Hendriks, H & Dellaglio, F 2004 Assessment of novel probiotic Lactobacillus casei strains for the production of functional dairy foods. International Dairy Journal 14 723736CrossRefGoogle Scholar
Boylston, TD, Vinderola, CG, Ghoddusi, HB & Reinheimer, JA 2004 Incorporation of bifidobacteria into cheeses: challenges and rewards. International Dairy Journal 14 375387CrossRefGoogle Scholar
Bradley, RL, Arnold, E, Barbano, DM, Semerad, RG, Smith, DE & Vines, BK 1993 Chemical and physical methods. In Standard methods for the examination of dairy product, pp. 433531 (Ed. Marshall, R). Washington: American Public Health AssociationGoogle Scholar
Corbo, MR, Albenzio, M, De Angelis, M, Sevi, A & Gobbetti, M 2001 Microbiological and biochemical properties of Canestrato Pugliese hard cheese supplemented with bifidobacteria. Journal of Dairy Science 84 (3) 551561CrossRefGoogle ScholarPubMed
Crow, VL, Curry, B & Hayes, M 2001 The ecology of non-starter lactic acid bacteria (NSLAB) and their use as adjuncts in New Zealand Cheddar. International Dairy Journal 11 275283CrossRefGoogle Scholar
Darukaradhya, J, Phillips, M & Kailasapathy, K 2006 Selective enumeration of Lactobacillus acidophilus, Bifidobacterium spp., starter lactic acid bacteria and non-starter lactic acid bacteria from Cheddar cheese. International Dairy Journal 16 (5) 439445CrossRefGoogle Scholar
FAO/OMS 2001 Informe de la consulta de expertos FAO/OMS sobre evaluación de las propiedades saludables y nutricionales de los probióticos en los alimentos, incluida la leche en polvo con bacterias vivas del ácido láctico. Córdoba, Argentina, 1 al 4 de octubre de 2001, pág 1–29Google Scholar
Gomes, AMP, Malcata, FX & Klaver, FAM 1998 Growth enhancement of Bifidobacterium lactis Bo and Lactobacillus acidophilus Ki by milk hydrolyzates. Journal of Dairy Science 81 28172825CrossRefGoogle Scholar
Gomes, AMP, Malcata, FX, Klaver, FAM & Grande, HJ 1995 Incorporation and survival of Bifidobacterium sp. Strain Bo and Lactobacillus acidophilus strain Ki in a cheese product. Netherlands Milk Dairy Journal 49 7195Google Scholar
Guardini, E 2008 Estadísticas lácteas argentinas. Resumen de las tablas de productos lácteos. Lácteos – Dirección de Industria Alimentaria – Secretaría de Agricultura, Ganadería, Pesca y Alimentos. http://www.sagpya.mecon.gov.ar/. Accessed March 9, 2009Google Scholar
Heller, KJ, Bockelmann, W, Schrezenmeir, J & de Vrese, M 2003 Cheese and its potential as a probiotic food. In Handbook of fermented functional foods, pp. 203225 (Ed. Farnworth, ER). Boca Raton: CRC PressCrossRefGoogle Scholar
Mc Brearty, S, Ross, R, Fitzgerald, G, Collins, J, Wallace, J & Stanton, C 2001 Influence of two commercially available bifidobacteria cultures on Cheddar cheese quality. International Dairy Journal 11 599610CrossRefGoogle Scholar
Milesi, MM, Candioti, M & Hynes, E 2007 Mini soft cheese as a simple model for biochemical studies on cheesemaking and ripening LWT – Food Science and Technology 40 (8) 14271433CrossRefGoogle Scholar
Ong, L, Henriksson, A & Shah, NP 2006 Development of probiotic Cheddar cheese containing Lactobacillus acidophilus, Lb. casei, Lb. paracasei and Bifidobacterium spp. and the influence of these bacteria on proteolytic patterns and production of organic acid. International Dairy Journal 16 446456CrossRefGoogle Scholar
Ong, L, Henriksson, A & Shah, NP 2007 Proteolytic pattern and organic acid profiles of probiotic Cheddar cheese as influenced by probiotic strains of Lactobacillus acidophilus, Lb. paracasei, Lb. casei or Bifidobacterium sp. International Dairy Journal 17 (1) 6778CrossRefGoogle Scholar
Phillips, M, Kailasapathy, K & Tran, L 2006 Viability of commercial probiotic cultures (L. acidophilus, Bifidobacterium sp.; L. casei, L. paracasei and L. rhamnosus) in Cheddar cheese. International Journal of Food Microbiology 108 276280CrossRefGoogle ScholarPubMed
Thomas, T 1987 Cannibalism among bacteria found in cheese. New Zealand Journal of Dairy Science and Technology 22 215219Google Scholar
Vinderola, CG & Reinheimer, JA 2000 Enumeration of Lactobacillus casei in the presence of L. acidophilus, bifidobacteria and lactic starter bacteria in fermented dairy products. International Dairy Journal 10 271275CrossRefGoogle Scholar
Williams, AG, Withers, SE & Banks, JM 2000 Energy sources of non-starter lactic acid bacteria isolated from Cheddar cheese. International Dairy Journal 10 1723CrossRefGoogle Scholar
Zalazar, CA, Meinardi, C & Hynes, E 1999 Los quesos argentinos. In Quesos típicos argentinos: Una revisión general sobre producción y características, pp. 2049. Santa Fe: Centro de Publicaciones Universidad Nacional del LitoralGoogle Scholar