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Oral administration of dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei delayed the progression of streptozotocin-induced diabetes in rats

Published online by Cambridge University Press:  12 May 2008

Hariom Yadav ,
Shalini Jain  and
Pushpalata Ravindra Sinha
Hariom Yadav*
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal – 132001Haryana, India Current address: Regenerative Biology Section, Diabetes Branch, National Institute of Diabetes & Digestive & Kidney Diseases, National Institutes of Health, Building 10, Clinical Research Center, West Laboratories, 5-5872, South Drive and Old Georgetown Road, Bethesda, MD20892
Shalini Jain
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal – 132001Haryana, India
Pushpalata Ravindra Sinha
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal – 132001Haryana, India
*
*For correspondence; e-mail: [email protected]
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Abstract

In this study, the effect of dahi containing probiotic Lactobacillus acidophilus NCDC14 and Lactobacillus casei NCDC19 (∼73×108 cfu/g) on progression of streptozotocin (STZ)-induced diabetes in rats (15 g/day/rat) for 28 days was investigated. Feeding of probiotic dahi significantly suppressed the incremental peaks and area under the curve and delayed reduction of insulin secretion during oral glucose tolerance test more than skim milk or control dahi. The feeding of milk products reduced the total cholesterol, triglycerides, LDL and VLDL-cholesterol and increased HDL-cholesterol levels (P<0·05). Moreover, probiotic dahi significantly suppressed STZ-induced oxidative damage in pancreatic tissues by inhibiting the lipid peroxidation and formation of nitric oxide, and preserving antioxidant pool such as glutathione content and activities of superoxide dismutase, catalase and glutathione peroxidase. The results suggest that the supplementation of probiotic Lb. acidophilus and Lb. casei with dahi cultures increased the efficacy of dahi to suppress STZ-induced diabetes in rats by inhibiting depletion of insulin as well as preserving diabetic dyslipidemia, and inhibiting lipid peroxidation and nitrite formation. This may empower antioxidant system of β-cells and may slow down the reduction of insulin and elevation of blood glucose levels.

Keywords

DiabetesdahiprobioticsantihyperlipidemicantioxidantLactobacillusmilkrat
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 2 , May 2008 , pp. 189 - 195
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Abbas, Z & Jafri, W 1992 Yoghurt (dahi): a probiotic and therapeutic view. Journal of Pakistan Medical Association 42 221–224Google Scholar
Agrawal, RAK & Kansal, VK 1991 Influence of skim milk on plasma lipids, lipoproteins and Lecithin: cholesterol acytransferase activity in cholesterol fed rabbits. Milchwissenschaft 46 355–357Google Scholar
Ahmed, RG 2005 The physiological and biochemical effects of diabetes on the balance of between oxidative stress and antioxidant defense system. Medical Journal of Islamic World of Academic Sciences 15 31–42Google Scholar
Aneja, RP, Mathur, BN, Chandan, RC & Benerjee, AK 2002 Dahi and Yogurt. In Technology of Indian milk products. pp. 160–169. DairyIndia yearbook (New Delhi, India)Google Scholar
Anuradha, CV & Selvam, R 1993 Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan induced diabetic rats. Journal of Nutritional Biochemistry 4 212–217CrossRefGoogle Scholar
Association of Official Analytical Chemists 1995 In Official methods of analysis (16th ed.). Gairthersburg: AOAC InternationalGoogle Scholar
Bruce, A, Freeman, D & James, C 1982 Biology of disease free radicals and tissue injury. Laboratory Investigations 47 412–426Google Scholar
Chance, B, Greenstein, DS & Roughton, RJW 1952 The mechanism of catalase action 1-steady state analysis. Archives in Biochemistry & Biophysics 37 301–339CrossRefGoogle Scholar
Chawla, K & Kansal, VK 1984 Effect of milk & its culture products on the plasma and organ lipids in rats. Indian Journal of Medical Research 79 418–421Google ScholarPubMed
Ellman, GL 1959 Tissue sulfhydryl groups. Achieves in Biochemistry & Biophysics 82 70–77CrossRefGoogle ScholarPubMed
FAO/WHO (2001) Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria. Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. http://www.who.int/foodsafety/publications/fs_management/probiotics/en/index.htmlGoogle Scholar
Folch, J, Lees, M & Sloane-Stanley, GH 1957 A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226 497–506.CrossRefGoogle ScholarPubMed
Friedewald, WT, Levi, RI & Fredrickson, DJ 1972 Estimation of the concentration of low density lipoprotein cholesterol in plasma without use of the ultracentrifuge. Clinical Chemistry 18 499–452CrossRefGoogle ScholarPubMed
Henry, RR 2001 Preventing cardiovascular complications of type 2 diabetes: focus on lipid management. Clinical Diabetes 19 113–120CrossRefGoogle Scholar
Klover, PJ & Mooney, RA 2004 Hepatocytes: critical for glucose homeostasis. International Journal of Biochemistry & Cell Biology 36 753–758CrossRefGoogle ScholarPubMed
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ 1951 Protein measurement with the Folin Phenol Reagent. Journal of Biological Chemistry 193 265–275CrossRefGoogle ScholarPubMed
Lyons, TJ 1991 Oxidized low density lipoproteins, a role in the pathogenesis of atherosclerosis in diabetes. Diabetes Medicine 8 411–419CrossRefGoogle ScholarPubMed
Marklund, S & Marklund, S 1974 Involvement of superoxide dismutase anion radical in autooxidation of pyrogallol and a convenient assay for super oxide dismutase. European Journal of Biochemistry 42 469–474CrossRefGoogle Scholar
Matsuzaki, T, Nagata, Y, Kado, S, Uchida, K, Hashimoto, S & Yokokura, T 1997c Effect of oral administration of Lactobacillus casei on alloxan-induced diabetes in mice. APMIS 105 637–642CrossRefGoogle ScholarPubMed
Matsuzaki, T, Nagata, Y, Kado, S, Uchida, K, Kato, I, Hashimoto, S & Yokokura, T 1997a Prevention of onset in an insulin-dependent diabetes mellitus model, NOD mice, by oral feeding of Lactobacillus casei. APMIS 105 643–649CrossRefGoogle Scholar
Matsuzaki, T, Yamazaki, R, Hashimoto, S & Yokokura, T 1997b Antidiabetic effects of an oral administration of Lactobacillus casei in a non-insulin-dependent diabetes mellitus (NIDDM) model using KK-Ay mice. Endocrinology Journal 44 357–365Google Scholar
McCord, JM, Keele, BB & Fridovich, I 1976 An enzyme based theory of obligate anaerobiosis; the physiological functions of super oxide dismutase. PNAS 68 1024–1027CrossRefGoogle Scholar
Moshage, H, Kok, B, Huizenga, JR & Jansen, PL 1995 Nitrite and nitrate determination in plasma: a crtical evaluation. Clinical Chemistry 41 892–896CrossRefGoogle Scholar
Ohkawa, H, Ohishi, N & Yagi, K 1979 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95 351–358CrossRefGoogle ScholarPubMed
Rajasekaran, S, Sivagnanam, K & Subramanian, S 2005 Antioxidant effect of Aloe vera gel extract in streptozotcin-indcued diabetes in rats. Pharmacological Reports 57 90–96Google ScholarPubMed
Reid, G, Anand, S, Bingham, MO, Mugua, G, Wadstrom, T, Fuller, R, Anukam, K & Katsivo, M 2005 Probiotics for the developing world. Journal of Clinical Gastroenterology 39 485–488CrossRefGoogle ScholarPubMed
Riezzo, G, Chiloiro, M & Russo, F 2005 Functional foods: salient features and clinical applications. Current Drug Targets in Immune and Endocrine Metabolic Disorders 5 331–337CrossRefGoogle ScholarPubMed
Roberfroid, MB 2000 Prebiotics and probiotics: are they functional foods. American Journal of Clinical Nutrition 71 1682S–1687SCrossRefGoogle ScholarPubMed
Robertson, RP & Harmon, JS 2006 Diabetes, glucose toxicity and oxidative stress: A case of double jeopardy for the pancreatic islet β-cells. Free Radical Biology and Medicine 41 177–184CrossRefGoogle Scholar
Rotruck, JT, Papeat, L, Ganther, HE & Swanson, AB 1973 Selenium biochemical role as a component of Glutathione peroxidase. Science 179 588–590CrossRefGoogle ScholarPubMed
Saionton, B 2002 Animal experimentation rules – Separating the reality from the rhetoric. Current Science 83 800–802Google Scholar
Sinha, AK 1972 Colorimetric assay of Catalase. Analytical Biochemistry 47 389–394CrossRefGoogle ScholarPubMed
Szkudelski, T 2001 The mechanism of alloxan and streptozotocin action in beta cells of the rat pancreas. Physiological Research 50 536–546Google Scholar
Tabuchi, M, Ozaki, M, Tamura, A, Yamada, N, Ishida, T, Hosoda, M & Hosono, A 2003 Antidiabetic effect of Lactobacillus GG in streptozotocin-induced diabetic rats. Bioscience, Biotechnology & Biochemistry 67 1421–1424CrossRefGoogle ScholarPubMed
Taylor, GRJ & Williams, CM 1998 Effects of probioics and prebiotics on blood lipids. British Journal of Nutrition 80 S225–S230CrossRefGoogle ScholarPubMed
Vander Vries, J 1954 Two methods for the determination of glycogen in liver. Biochemistry Journal 57 410–416CrossRefGoogle Scholar
Wild, S, Roglic, G & Green, A 2004 Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 27 1047–1053CrossRefGoogle ScholarPubMed
Wilson, GL, Patton, NJ, McCord, JM, Millins, DW & Mossman, BT 1984 Mechanisms of streptozotocin and alloxan induced damage in rat beta cells. Diabetologia 27 587–596CrossRefGoogle Scholar
Yadav, H, Jain, S & Sinha, PR 2005 Preparation of low fat dahi. Journal of Dairying Food & Home Sciences 24 172–177Google Scholar
Yadav, H, Jain, S & Sinha, PR 2007a Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus, Lactobacillus casei and Lactococcus lactis bacteria in high fructose diet fed rats. Nutrition 72 62–68CrossRefGoogle Scholar
Yadav, H, Jain, S & Sinha, PR 2007b Formation of oligosaccharides in skim milk fermented with mixed dahi cultures, Lactococcus lactis ssp diacetylactis and probiotic strains of lactobacilli. Journal of Dairy Research 74 154–159CrossRefGoogle ScholarPubMed
Yadav, H, Jain, S & Sinha, PR 2007c Production of free fatty acids and conjugated linoleic acid in probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei during fermentation and storage. International Dairy Journal 60 1006–1010CrossRefGoogle Scholar