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Neutrophil and CD4+ milk cell count related to natural incidence of mastitis in Jersey cattle

Published online by Cambridge University Press:  08 July 2021

Zlatina Chengolova ,
Milka Atanasova  and
Tzonka Godjevargova
Zlatina Chengolova*
Affiliation:
Biotechnology Department, “Prof. Dr Asen Zlatarov” University of Burgas, Burgas, Bulgaria
Milka Atanasova
Affiliation:
Biotechnology Department, “Prof. Dr Asen Zlatarov” University of Burgas, Burgas, Bulgaria
Tzonka Godjevargova
Affiliation:
Biotechnology Department, “Prof. Dr Asen Zlatarov” University of Burgas, Burgas, Bulgaria
*
Author for correspondence: Zlatina Chengolova, Email: [email protected]
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Abstract

This Research Communication describes the relation between somatic cells and microbial content in milk from Jersey cattle. Milk samples were classified in groups: healthy, dirty and mastitic (from Staphylococcus spp., Escherichia coli, Coliforms). The somatic cells in each of those groups were analysed by two methods – flow cytometric and automatic fluorescent cell counting. Those methods were compared. Total somatic cell count (SCC), neutrophil count, and lymphocytes with cluster of differentiation 4 (CD4+cells) were determined. There was a positive relationship between microbes and somatic cells. It was noticed that the neutrophil count was generally increased together with SCC, whilst the CD4+ cell count was higher in healthy milk samples (about 8%) compared to mastitic ones (about 3%). Lower number of CD4+ cells (from 1 to 4%) was determined in samples positive for Staphylococcus spp. but with lower SCC (from 2.7 to 4.0 × 105 cells/ml). Also, the number of CD4+ cells in Staphylococcus spp.-positive samples increased (to 4.8%) together with higher SCC, something that was not observed in the other mastitic samples. Knowledge of those relations could be useful for veterinary medical tests in the initial phase of inflammation.

Keywords

CD4+ cellsmastitisneutrophilssomatic cell countStaphylococcus aureus
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 334 - 336
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Alhussien, MN and Dang, AK (2020) Sensitive and rapid lateral-flow assay for early detection of subclinical mammary infection in dairy cows. Scientific Reports 10, 11161.CrossRefGoogle ScholarPubMed
Becheva, Z, Gabrovska, K and Godjevargova, T (2017) Immunofluorescence microscope assay of neutrophils and somatic cells in bovine milk. Food and Agricultural Immunology 28, 1196–1210.CrossRefGoogle Scholar
Blagitz, MG, Souza, FN, Batista, CF, Azevedo, LFF, Benites, NR, Melville, PA, Della Libera, AMMP, et al. (2015) The neutrophil function and lymphocyte profile of milk from bovine mammary glands infected with Streptococcus dysgalactiae. Journal of Dairy Research 82, 460–469.CrossRefGoogle ScholarPubMed
Chengolova, Z, Ivanov, Y and Grigorova, G (2021) The relationship of bovine milk somatic cell count to neutrophil level in samples of cow's milk assessed by an automatic cell counter. Journal of Dairy Research 88, In press.Google Scholar
Cobirka, M, Tancin, V and Slama, P (2020) Epidemiology and classification of mastitis. Animals 10, 2212.CrossRefGoogle ScholarPubMed
Goldberg, JJ, Pankey, JW, Politis, I, Zavizion, B and Bramley, AJ (1995) Effect of oxygen tension on killing of Escherichia coli by bovine polymorphonuclear neutrophil leucocytes in vitro. Journal of Dairy Research 62, 331–338.CrossRefGoogle ScholarPubMed
Hohmann, MF, Wente, N, Zhang, Y and Krömker, V (2020) Bacterial load of the teat apex skin and associated factors at herd level. Animals 10, 1647.CrossRefGoogle ScholarPubMed
Newbould, FH (1970) Enhancement of phagocytosis in bovine milk leukocytes in vitro. Canadian Journal of Comparative Medicine 34, 261–264.Google ScholarPubMed
Putz, EJ, Palmer, MV, Ma, H, Casas, E, Reinhardt, TA and Lippolis, JD (2020) Case report: characterization of a persistent, treatment-resistant, novel Staphylococcus aureus infection causing chronic mastitis in a Holstein dairy cow. BMC Veterinary Research 16, 1–8.CrossRefGoogle Scholar
Rainard, P, Foucras, G, Boichard, D and Rupp, R (2018) Invited review: low milk somatic cell count and susceptibility to mastitis. Journal of Dairy Science 101, 6703–6714.CrossRefGoogle ScholarPubMed
Rasheed, A, Usman, T and Niaz, K (2020) A review on bovine mastitis with special focus on CD4 as a potential candidate gene for mastitis resistance: a review. Annals of Animal Science 20, 735–755.CrossRefGoogle Scholar
Taylor, BC, Keefe, RG, Dellinger, JD, Nakamura, Y, Cullor, JS and Stott, JL (1997) T cell populations and cytokine expression in milk derived from normal and bacteria-infected bovine mammary glands. Cellular Immunology 182, 68–76.CrossRefGoogle ScholarPubMed
Yang, JD (2018) Antigen Specific CD4 + and CD8 + T Cell Recognition During Mycobacterium tuberculosis Infection (GSBS dissertations and theses). https://doi.org/10.13028/M27M3N.CrossRefGoogle Scholar
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