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Modification of the volatile compound profile of cheese, by a Lactococcus lactis strain expressing a mutant oligopeptide binding protein

Published online by Cambridge University Press:  29 January 2008

Antonia Picon ,
Estrella Fernández-García ,
Pilar Gaya  and
Manuel Nuñez
Antonia Picon
Affiliation:
Departamento de Tecnología de Alimentos, SGIT-INIA, Carretera de La Coruña Km 7.5, 28040 MadridSpain
Estrella Fernández-García*
Affiliation:
Departamento de Tecnología de Alimentos, SGIT-INIA, Carretera de La Coruña Km 7.5, 28040 MadridSpain
Pilar Gaya
Affiliation:
Departamento de Tecnología de Alimentos, SGIT-INIA, Carretera de La Coruña Km 7.5, 28040 MadridSpain
Manuel Nuñez
Affiliation:
Departamento de Tecnología de Alimentos, SGIT-INIA, Carretera de La Coruña Km 7.5, 28040 MadridSpain
*
*For correspondence; e-mail: [email protected]
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Abstract

Lactococcus lactis strain AMP2I expresses OppA(D471R), a mutant oligopeptide binding OppA protein in which the aspartyl residue at position 471 was replaced by arginine. As a consequence of a different peptide transport in this strain, experimental Hispánico cheese made with Lc. lactis AMP2I had a higher content of total free amino acids than control cheese made with Lc. lactis AMP1I, an isogenic strain expressing wild-type OppA (Picon et al. 2005, 2007). In this work higher levels of diketones, hydroxy-ketones and, to a lesser extent, branched chain aldehydes were recorded for experimental cheese compared with control cheese. These differences levelled off as ripening proceeded. Strong correlations support the hypothesis that the increased levels of these volatile compounds in cheese made with Lc. lactis AMP2I are linked to higher concentrations of free amino acids threonine, valine and leucine.

Keywords

Oligopeptide binding protein (OppA)Hispánico cheeseVolatile compounds
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 1 , February 2008 , pp. 30 - 36
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Arfi, K, Landaud, S & Bonnarme, P 2006 Evidence for distinct L-methionine catabolic pathways in the yeast Geotrichum candidum and the bacterium Brevibacterium linens. Applied and Environmental Microbiology 72 2155–2162CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists 1984 In Official Methods of Analysis, 14th ed (Ed. Williams, S). Washington, DC, USA: AOACGoogle Scholar
Curioni, PMG & Bosset, JO 2002 Key odorants in various cheese types as determined by gas chromatography-olfactometry. International Dairy Journal 12 959–984CrossRefGoogle Scholar
Fernández-García, E, Carbonell, M & Nuñez, M 2002 Volatile fraction and sensory characteristics of Manchego cheese, 1. Comparison of raw and pasteurised milk cheese. Journal of Dairy Research 69 579–593CrossRefGoogle Scholar
Fox, PF & Wallace, JM 1997 Formation of flavour compounds in cheese. Advances in Applied Microbiology 45 17–85CrossRefGoogle ScholarPubMed
Garde, S, Carbonell, M, Fernández-García, E, Medina, M & Nuñez, M 2002 Volatile compounds in Hispánico cheese manufactured using a mesophilic starter, a thermophilic adjunct culture, and bacteriocin-producing Lactococcus lactis subsp. lactis INIA 415. Journal of Agricultural and Food Chemistry 50 6752–6757CrossRefGoogle Scholar
Kieronczyk, A, Skeie, S, Langsrud, T, Le Bars, D & Yvon, M 2004 The nature of aroma compounds produced in a cheese model by glutamate dehydrogenase positive Lactobacillus INF15D depends on its relative aminotransferase activities towards the different amino acids. International Dairy Journal 14 227–235CrossRefGoogle Scholar
Kunji, ERS, Mierau, I, Hagting, A, Poolman, B & Konings, WN 1996 The proteolytic system of lactic acid bacteria. Antonie van Leeuwenhoek 70 187–221CrossRefGoogle ScholarPubMed
Lees, GJ & Jago, GR 1976 Formation of acetaldehyde from threonine by lactic acid bacteria. Journal of Dairy Research 43 75–83CrossRefGoogle ScholarPubMed
Molimard, P & Spinnler, HE 1996 Review: Compounds involved in the flavor of surface mold-ripened cheeses: origins and properties. Journal of Dairy Science 79 169–184CrossRefGoogle Scholar
Ott, A, Germond, JE & Chaintreau, A 2000 Vicinal diketone formation in yogurt: 13C precursors and effect of branched-chain amino acids. Journal of Agriculture and Food Chemistry 48 724–731CrossRefGoogle ScholarPubMed
Picon, A, Kunji, ERS, Lanfermeijer, FC, Konings, WN & Poolman, B 2000 Specificity mutants of the binding protein of the oligopeptide transport system of Lactococcus lactis. Journal of Bacteriology 182 1600–1608CrossRefGoogle ScholarPubMed
Picon, A, de Torres, B, Gaya, P & Nuñez, M 2005 Cheesemaking with a Lactococcus lactis strain expressing a mutant oligopeptide binding protein as starter results in a different peptide profile. International Journal of Food Microbiology 104 299–307CrossRefGoogle Scholar
Picon, A, Gaya, P & Nuñez, M 2007 Lowering hydrophobic peptides and increasing free amino acids in cheese made with a Lactococcus lactis strain expressing a mutant oligopeptide binding protein. International Dairy Journal 17 218–225CrossRefGoogle Scholar
Ramos, A, Jordan, KN, Cogan, TM & Santos, H 1994 13C nuclear magnetic resonance studies of citrate and glucose co-metabolism by Lactococcus lactis. Applied and Environmental Microbiology 60 1739–1748CrossRefGoogle Scholar
Sablé, S & Cottenceau, G 1999 Current knowledge of soft cheeses flavor and related compounds. Journal of Agricultural and Food Chemistry 47 4825–4836CrossRefGoogle ScholarPubMed
Singh, TK, Drake, MA & Cadwallader, KR 2003 Flavor of Cheddar cheese: a chemical and sensory perspective. Comprehensive Reviews in Food Science and Food Safety 2 166–189CrossRefGoogle ScholarPubMed
Smit, G, Smit, BA & Engels, WJM 2005 Flavour formation by lactic acid bacteria and biochemical flavour profiling of cheese products. FEMS Microbiology Reviews 29 591–610CrossRefGoogle ScholarPubMed