Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-23T09:43:41.241Z Has data issue: false hasContentIssue false
  • English
  • Français

Milk fatty acid variability: effect of some candidate genes involved in lipid synthesis

Published online by Cambridge University Press:  11 March 2013

Cinzia Marchitelli ,
Giovanna Contarini ,
Giovanna De Matteis ,
Alessandra Crisà ,
Lorraine Pariset ,
Maria Carmela Scatà ,
Gennaro Catillo ,
Francesco Napolitano  and
Bianca Moioli
Cinzia Marchitelli
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Giovanna Contarini
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Lombardo 19, 26900 Lodi, Italy
Giovanna De Matteis
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Alessandra Crisà
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Lorraine Pariset
Affiliation:
University of Tuscia, via de Lellis, 01100 Viterbo, Italy
Maria Carmela Scatà
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Gennaro Catillo
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Francesco Napolitano
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Bianca Moioli*
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In this work, the genetic variation of milk FA was investigated in three different bovine breeds, the Jersey, the Piedmontese and the Valdostana, and at different lactation stages. All animals were genotyped for 21 Single Nucleotide Polymorphisms located within nine candidate genes involved in lipid synthesis: diacylglycerol acyltransferase 1 and 2 (DGAT1, 2); stearoyl-CoA desaturase (SCD); growth hormone receptor (GHR); fatty acid synthase (FASN); acyl-CoA dehydrogenase (ACAD); fatty acid binding protein (FABP4); lipoprotein lipase (LPL); and leptin gene (LEP). The highest milk-fat Jersey breed also showed the highest content of saturated FA. Throughout lactation, the breeds showed a similar variation in the FA, with a decrease in the short-chain, this was accompanied by a general increase in the long chain FA at the end of lactation. The increase in long chain saturated FA was particularly evident in the case of the Jersey. The effect of SCD gene on the C14 desaturation index was confirmed; the DGAT1 gene was polymorphic only in the Jersey breed, but its effect was confirmed only on milk fat content; three further potential candidate genes were identified: first, the FABP4 gene, which was found to influence medium and long chain FA in all the breeds, but not the desaturation indices; second, the FASN gene, which was found to influence the amount of PUFA in the Piedmontese and the Valdostana, and third, the LPL gene, which was found to affect fat content in the Piedmontese.

Keywords

Milk fatty acidsSingle Nucleotide PolymorphismCandidate genes
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 2 , May 2013 , pp. 165 - 173
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barendse, W, Harrison, BE, Bunch, RJ & Thomas, MB 2008 Variation at the Calpain 3 gene is associated with meat tenderness in zebu and composite breeds of cattle. BMC Genetics 9 41Google Scholar
Blott, S, Kim, JJ, Moisio, S, Schmidt-Kuntzel, A, Cornet, A, Berzi, P, Cambisano, N, Ford, C, Grisart, B, Johnson, D, Karim, L, Simon, P, Snell, R, Spelman, R, Wong, J, Vilkki, J, Georges, M, Farnir, F & Coppieters, W 2003 Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition. Genetics 163 253–266Google Scholar
Bouwman, AC, Bovenhuis, H, Visker, MH & van Arendok, JA 2011 Genome-wide association of milk fatty acids in Dutch dairy cattle. BMC Genetics 12 43Google Scholar
Buchanan, FC, Van Kessel, AG, Waldner, C, Christensen, DA, Laarveld, B & Schumutz, SM 2003 Hot topic: an association between a leptin single nucleotide polymorphism and milk and protein yield. Journal of Dairy Science 86 3164–3166CrossRefGoogle ScholarPubMed
Cases, S, Stone, SJ, Zhou, P, Yen, E, Tow, B, Lardizabal, KD, Voelker, T & Farese, RV 2001 Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members. Journal of Biological Chemistry 276 38870–38876CrossRefGoogle ScholarPubMed
Conte, G, Mele, M, Chessa, S, Castiglioni, B, Serra, A, Pagnacco, G & Secchiari, P 2010 Dyacylglycerol acyltransferase 1, stearoyl-CoA desaturase 1, and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle. Journal of Dairy Science 93 753–763Google Scholar
Ding, XZ, Liang, CN, Guo, X, Xing, CF, Bao, CJ, Chu, M, Pei, J, Zhu, XS & Yan, P 2012 A novel single nucleotide polymorphism in exon 7 of LPL gene and its association with carcass traits and visceral fat deposition in yak (Bos grunniens) steers. Molecular Biology Reports 39 669–673Google Scholar
Garcia-Fernandez, M, Gutierrez-Gil, B, Garcia-Gamez, E, Sanchez, JP & Arranz, JJ 2010 The identification of QTL that affect the fatty acid composition of milk on sheep chromosome 11. Animal Genetics 41 324–328CrossRefGoogle ScholarPubMed
Grisart, B, Farnir, F, Karim, L, Cambisano, N, Kim, JJ, Kvasz, A, Mni, M, Simon, P, Frère, JM, Coppieters, W & Georges, M 2004 Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition. Proceedings of the National Academy of Sciences of the United States of America 101 2398–403Google Scholar
Hulshof, KF, van Erp-Baart, MA, Anttolainen, M, Becker, W, Church, SM, Couet, C, Hermann-Kunz, E, Kesteloot, H, Leth, T, Martins, I, Moreiras, O, Moschandreas, J, Pizzoferrato, L, Rimestad, AH, Thorgeirsdottir, H, van Amelsvoort, JM, Aro, A, Kafatos, AG, Lanzmann-Petithory, D & van Poppel, G 1999 Intake of fatty acids in western Europe with emphasis on trans fatty acids: the TRANSFAIR Study. European Journal of Clinical Nutrition 53 143–57Google Scholar
ISO (2001) Milk and milk products – extraction methods for lipids and liposoluble compounds. International Organization for Standardization: Geneva, Switzerland International Standard ISO 14156Google Scholar
ISO (2002) Milk fat: preparation of fatty acid methyl esters. International Organization for Standardization: Geneva, Switzerland International Standard ISO 15884Google Scholar
Kaneda, M, Linz, BZ, Sasazaki, S, Oyama, K & Mannen, H 2011 Allele frequencies of gene polymorphisms related to economic traits in Bos Taurus and Bos indicus cattle breeds. Journal of Animal Science 82 717–721Google Scholar
Karijord, O, Standal, N& Syrstad, O 1982 Sources of variation in composition of milk fat. Zeitschrift für Tierzüchtung und Züchtungsbiologie 99 81–93CrossRefGoogle Scholar
Lawless, F, Stanton, C, Escop, P, Devery, R, Dillon, P & Murphy, JJ 1999 Influence of breed on bovine milk cis-9, trans-11-conjugated linoleic acid content. Livestock Production Science 62 43–49CrossRefGoogle Scholar
Lea, W, Abbas, AS, Sprecher, H, Vockley, J & Schulz, H 2000 Long-chain acyl-CoA dehydrogenase is a key enzyme in the mitochondrial beta-oxidation of unsaturated fatty acids. Biochimica et Biophysica Acta 1485 121–128Google Scholar
Madeja, Z, Adamowicz, T, Chmurzynska, A, Jankowski, T, Melonek, J, Switonski, M & Strabel, T 2004 Short communication: effect of leptin gene polymorphism on breeding value for milk production traits. Journal of Dairy Science 87 3925–3927Google Scholar
Manuguerra, M, Matullo, G, Veglia, F, Autrup, H, Dunning, AM, Garte, S, Gormally, E, Malaveille, C, Guarrera, S, Polidoro, S, Saletta, F, Peluso, M, Airoldi, L, Overvad, K, Raaschou-Nielsen, O, Clavel-Chapelon, F, Linseisen, J, Boeing, H, Trichopoulos, D, Kalandidi, A, Palli, D, Krogh, V, Tumino, R, Panico, S, Bueno De-Mesquita, HB, Peeters, PH, Lund, E, Pera, G, Martinez, C, Amiano, P, Barricarte, A, Tormo, MJ, Quiros, JR, Berglund, G, Janzon, L, Jarvholm, B, Day, NE, Allen, N, Saracci, R, Kaaks, R, Ferrari, P, Riboli, E & Vineis, P 2007 Multi-factor dimensionality reduction applied to a large prospective investigation on gene-gene and gene-environment interactions. Carcinogenesis 28 414–422Google Scholar
Matsuhashi, T, Maruyama, S, Uemoto, Y, Kobayashi, N, Mannen, H, Abe, T, Sakaguchi, S & Kobayashi, E 2011 Effects of bovine fatty acid synthase, stearoyl-coenzyme A desaturase, sterol regulatory element-binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle. Journal of Animal Science 12 22–89Google Scholar
Mechanic, LE, Luke, BT, Goodman, JE, Chanock, SJ & Harris, CC 2008 Polymorphism Interaction Analysis (PIA): a method for investigating complex gene-gene interactions. BMC Bioinformatics 9 146Google Scholar
Michal, JJ, Zhang, ZW, Gaskins, CT & Jiang, Z 2006 The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu×Limousin F2 crosses. Animal Genetics 37 400–402Google Scholar
Moioli, B, Contarini, G, Avalli, A, Catillo, G, Orru, L, De Matteis, G, Masoero, G & Napolitano, F 2007 Short communication: effect of stearoyl-coenzyme A desaturase polimorphism on fatty acid composition of milk. Journal of Dairy Science 90 3553–3558Google Scholar
Roy, R, Ordovas, L, Zaragoza, P, Romero, A, Moreno, C, Altarriba, J & Rodellar, C 2006 Association of polymorphisms in the bovine FASN gene with milk-fat content. Animal Genetics 37 215–218Google Scholar
SAS Institute. 2007 SAS/STAT User's Guide. Version 9.1. Cary, NC: SAS Institute Inc.Google Scholar
Schennink, A, Stoop, WM, Visker, MHPW, Heck, JML, Bovenhuis, H, van der Poel, JJ, van Valenberg, HJF & van Arendonk, JAM 2007 DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Animal Genetics 38 467–473Google Scholar
Schennink, A, Heck, JML, Bovenhuis, H, Visker, MHPW, van Valenberg, HJF & van Arendonk, JAM 2008 Milk fatty acid unsaturation: genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and acyl CoA:diacylglycerol acyltransferase 1 (DGAT1). Journal of Dairy Science 91 2135–2143Google Scholar
Sherman, EL, Nkrumah, JD, Murdoch, BM, Li, C, Wang, Z, Fu, A & Moore, S 2008 Polymorphisms and haplotypes in the bovine NPY, GHR, GHRL, IGF2, UCP2, and UCP3 genes and their associations with measures of growth, performance, feed efficiency and carcass merit in beef cattle. Journal of Animal Science 86 1–16Google Scholar
Stoop, WM, Schennink, A, Visker, MHPW, Mullaart, E, van Arendonk, JAM & Bovenhuis, H 2009 Genome-wide scan for bovine milk-fat composition. I. Quantitative trait loci for short and medium-chain fatty acids. Journal of Dairy Science 92 4664–4675Google Scholar
Taniguchi, M, Utsugi, T, Oyama, K, Mannen, H, Kobayashi, M, Tanabe, Y, Ogino, A & Tsuji, S 2004 Genotype of Stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle. Mammalian Genome 14 142–148Google Scholar
Viitala, S, Szyda, J, Blott, S, Schulman, N, Lidauer, M, Maki-Tanila, A, Georges, M & Vilkki, J 2006 The role of the bovine growth hormone receptor and prolactin receptor genes in milk, fat and protein production in Finnish Ayrshire dairy cattle. Genetics 173 2151–2164CrossRefGoogle ScholarPubMed
Williams, JL, Dunner, S, Valentini, A, Mazza, R, Amarger, V, Checa, ML, Crisà, A, Razzaq, N, Delourme, D, Grandjean, F, Marchitelli, C, Garcia, D, Perez Gomez, R, Negrini, R, Ajmone Marsan, P & Leveziel, H 2009 Discovery, characterization and validation of single nucleotide polymorphisms within 206 bovine genes that may be considered as candidate genes for beef production and quality. Animal Genetics 40 486–491CrossRefGoogle ScholarPubMed
Winter, A, van Eckeveld, M, Bininda-Emonds, ORP, Habermann, FA & Fries, R 2003 Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals. Cytogenetic and Genome Research 102 42–47CrossRefGoogle ScholarPubMed