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Milk cathelicidin and somatic cell counts in dairy goats along the course of lactation

Published online by Cambridge University Press:  03 June 2019

Vittorio Tedde ,
Valerio Bronzo ,
Giulia Maria Grazia Puggioni ,
Claudia Pollera ,
Antonio Casula ,
Giulio Curone ,
Paolo Moroni ,
Sergio Uzzau  and
Maria Filippa Addis
Vittorio Tedde
Affiliation:
Porto Conte Ricerche, Alghero, Italy
Valerio Bronzo
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy
Giulia Maria Grazia Puggioni
Affiliation:
Porto Conte Ricerche, Alghero, Italy
Claudia Pollera
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy
Antonio Casula
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy
Giulio Curone
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy
Paolo Moroni
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy Cornell University, Animal Health Diagnostic Center, Ithaca, NY, USA
Sergio Uzzau
Affiliation:
Porto Conte Ricerche, Alghero, Italy Dipartimento di Scienze Biomediche, Università degli Studi di Sassari, Sassari, Italy
Maria Filippa Addis*
Affiliation:
Porto Conte Ricerche, Alghero, Italy Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy
*
Author for correspondence: Maria Filippa Addis, Email: [email protected]
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Abstract

This research communication reports the evaluation of cathelicidin in dairy goat milk for its relationship with the somatic cell count (SCC) and microbial culture results. Considering the limited performances of SCC for mastitis monitoring in goats, there is interest in evaluating alternative diagnostic tools. Cathelicidin is an antimicrobial protein involved in innate immunity of the mammary gland. In this work, half-udder milk was sampled bimonthly from a herd of 37 Alpine goats along an entire lactation and tested with the cathelicidin ELISA together with SCC and bacterial culture. Cathelicidin and SCC showed a strong correlation (r = 0.72; n = 360 milk samples). This was highest in mid-lactation (r = 0.83) and lowest in late lactation (r = 0.61), and was higher in primiparous (0.80, n = 130) than in multiparous goats (0.71, n = 230). Both markers increased with stage of lactation, but cathelicidin increased significantly less than SCC. In addition, peak level in late lactation was lower for cathelicidin (5.05-fold increase) than for SCC (7.64-fold increase). Twenty-one (5.8%) samples were positive to bacteriological culture, 20 for coagulase-negative staphylococci and one for Streptococcus spp.; 18 of them were positive to the cathelicidin ELISA (85.71% sensitivity). Sensitivity of SCC >500 000 and of SCC >1 000 000 cells/ml was lower (71.43 and 23.81%, respectively). Therefore, the high correlation of cathelicidin with SCC during the entire lactation, along with its lower increase in late lactation and good sensitivity in detecting intramammary infection (IMI), indicate a potential for monitoring subclinical mastitis in dairy goats. However, based on this preliminary assessment, specificity should be improved (40.41% for cathelicidin vs. 54.57 and 67.85% for SCC >500 000 and >1 000 000 cells/ml, respectively). Therefore, the application of cathelicidin for detecting goat IMI will require further investigation and optimization, especially concerning the definition of diagnostic thresholds.

Keywords

Cathelicidindairy goatsELISAsomatic cell countssubclinical mastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 217 - 221
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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