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Metabolism of [U-14C; 2, 3-3H]-L-valine by the isolated perfused goat udder

Published online by Cambridge University Press:  01 June 2009

Eddy Roets ,
Anne-Marie Massart-LeëN ,
Roger Verbeke  and
Georges Peeters
Eddy Roets
Affiliation:
Department of Physiology, Faculty of the University of Ghent, Casinoplein 24, B-9000 Ghent, Belgium
Anne-Marie Massart-LeëN
Affiliation:
Department of Physiology, Faculty of the University of Ghent, Casinoplein 24, B-9000 Ghent, Belgium
Roger Verbeke
Affiliation:
Department of Physiology, Faculty of the University of Ghent, Casinoplein 24, B-9000 Ghent, Belgium
Georges Peeters
Affiliation:
Department of Physiology, Faculty of the University of Ghent, Casinoplein 24, B-9000 Ghent, Belgium
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Summary

Two lactating mammary glands excised from 2 goats were perfused for several hours in the presence of [U-14C; 2,3-3H]-L-valine and received adequate quantities of glucose, acetate and amino acids. In the synthesized milk 96 and 89% respectively of the casein valine was derived from free plasma valine. Valine was extensively catabolized by mammary tissue, resulting in a considerable 14CO2 production and in the incorporation of 14C into milk citric acid and to a lesser extent into casein aspartic acid and glutamic acid. About 30% of the valine molecules which were taken up by the mammary gland were oxidized to CO2 and 70% were incorporated in casein as valine residues. About 10% of the plasma valine molecules were reversibly transaminated during one passage through the udder. An important amount of radioactivity of plasma was present in unknown metabolites. Only 7% of this activity was localized in isobutyrate. The radioactivity of total milk fat was very low. Mainly iso-14:0, iso-16:0 and 15:0 were labelled.

Type
Original Articles
Information
Journal of Dairy Research , Volume 46 , Issue 1 , January 1979 , pp. 47 - 57
Copyright
Copyright © Proprietors of Journal of Dairy Research 1979

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