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Leucocytes of sheep colostrum, milk and involution secretion, with particular reference to ultrastructure and lymphocyte sub-populations

Published online by Cambridge University Press:  01 June 2009

Chee-Seong Lee  and
Peter M. Outteridge
Chee-Seong Lee
Affiliation:
Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria 3052, Australia
Peter M. Outteridge
Affiliation:
C.S.I.R.O., Division of Animal Health, Parkville, Victoria 3052, Australia
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Summary

The leucocytes in sheep colostrum, milk and involution secretion were studied using the electron microscope. The predominant cell type in colostrum was the polymorphonuclear leucocyte (PMNL) (41–84%), followed by the macrophage (8–49%), and the lymphocyte (6–11 %). Plasma cells were present in low numbers (1–2%) while no secretory epithelial cells were observed. In mid-lactation the cell components changed so that the macrophage was the predominant cell (83–86%) followed by the lymphocyte (10–17%). In early involution secretion, PMNL reappeared but declined in secretion obtained 21 d after weaning, when the macrophage was again the predominant cell. Associated with the whole cells were membranous extracellular materials and ‘sunburst’ fragments of the secretory epithelial cells. These were phagocytosed by both PMNL and macrophages, which also engulfed fat droplets from the milk. The lymphocytes were examined for plasma membrane markers and the T-cell percentage (E+ rosettes) fluctuated within wide limits, (0–80 %). However, there was no discernible trend associated with stage of lactation. The B-cell markers (C1 and Fcγ) also fluctuated widely and it was evident that contaminating monocytes reduced the accuracy of the count. However, it appeared that more B-cells were present in milk than in blood.

Type
Original Articles
Information
Journal of Dairy Research , Volume 48 , Issue 2 , June 1981 , pp. 225 - 237
Copyright
Copyright © Proprietors of Journal of Dairy Research 1981

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