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Isolation and quantification of cholesterol oxides in dairy products by selected ion monitoring mass spectrometry

Published online by Cambridge University Press:  01 June 2009

Jacob H. Nielsen ,
Carl Erik Olsen ,
Christina Duedahl  and
Leif H. Skibsted
Jacob H. Nielsen
Affiliation:
KVL Centre of Food Research, Department of Dairy and Food Science and Chemistry Department, Royal Veterinary and Agricultural University Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark
Carl Erik Olsen
Affiliation:
KVL Centre of Food Research, Department of Dairy and Food Science and Chemistry Department, Royal Veterinary and Agricultural University Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark
Christina Duedahl
Affiliation:
KVL Centre of Food Research, Department of Dairy and Food Science and Chemistry Department, Royal Veterinary and Agricultural University Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark
Leif H. Skibsted*
Affiliation:
KVL Centre of Food Research, Department of Dairy and Food Science and Chemistry Department, Royal Veterinary and Agricultural University Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark
*
*For correspondence.
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Summary

A method for isolation, detection and quantification of cholesterol oxidation products based on solid phase extraction in combination with preparative HPLC and gas chromatography–mass spectrometry selected ion monitoring has been developed for dairy products. The isolation procedure had a high recovery and artifact formation was minimal, as shown by isotope labelling. The limits of detection ranged from 0.3 to 35 pg/μl of the isomeric forms of 7-hydroxycholesterol, 20α-hydroxycholesterol, the isomeric forms of cholesterol-5,6-epoxides, cholestanetriol, 25-hydroxycholesterol and 7-ketocholesterol corresponding to a limit of quantification of 2–6 ng oxysterol/g lipid in the dairy product, depending on the nature of the cholesterol oxidation product.

Type
Original articles
Information
Journal of Dairy Research , Volume 62 , Issue 1 , February 1995 , pp. 101 - 113
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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References

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