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Isolation and characterisation of lactic acid bacteria from donkey milk

Published online by Cambridge University Press:  07 September 2016

Maria de los Dolores Soto del Rio ,
Christian Andrighetto ,
Alessandra Dalmasso ,
Angiolella Lombardi ,
Tiziana Civera  and
Maria Teresa Bottero
Maria de los Dolores Soto del Rio
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Christian Andrighetto
Affiliation:
Veneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, Via San Gaetano 74, 36016 Thiene (VI), Italy
Alessandra Dalmasso
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Angiolella Lombardi
Affiliation:
Veneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, Via San Gaetano 74, 36016 Thiene (VI), Italy
Tiziana Civera*
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Maria Teresa Bottero
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

During the last years the interest in donkey milk has increased significantly mainly because of its compelling functional elements. Even if the composition and nutritional properties of donkey milk are known, its microbiota is less studied. This Research Communication aimed to provide a comprehensive characterisation of the lactic acid bacteria in raw donkey milk. RAPD-PCR assay combined with 16S rDNA sequencing analysis were used to describe the microbial diversity of several donkey farms in the North West part of Italy. The more frequently detected species were: Lactobacillus paracasei, Lactococcus lactis and Carnobacterium maltaromaticum. Less abundant genera were Leuconostoc, Enterococcus and Streptococcus. The yeast Kluyveromyces marxianus was also isolated. The bacterial and biotype distribution notably diverged among the farms. Several of the found species, not previously detected in donkey milk, could have an important probiotic activity and biotechnological potential. This study represents an important insight to the ample diversity of the microorganisms present in the highly selective ecosystem of raw donkey milk.

Keywords

Donkey milklactic acid bacteriaRAPD-PCR characterisation
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 383 - 386
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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