Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-23T14:03:48.241Z Has data issue: false hasContentIssue false
  • English
  • Français

Intramammary infusion of a live culture of Lactococcus lactis for treatment of bovine mastitis: comparison with antibiotic treatment in field trials

Published online by Cambridge University Press:  04 August 2008

Katja Klostermann ,
Fiona Crispie ,
James Flynn ,
R Paul Ross ,
Colin Hill  and
William Meaney
Katja Klostermann
Affiliation:
Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co.Cork, Ireland Department of Microbiology, University College Cork, Cork, Ireland
Fiona Crispie
Affiliation:
Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co.Cork, Ireland
James Flynn
Affiliation:
Dairy Production Research Centre, Teagasc, Moorepark, Fermoy, Co.Cork, Ireland
R Paul Ross*
Affiliation:
Moorepark Food Research Centre, Teagasc, Moorepark, Fermoy, Co.Cork, Ireland
Colin Hill
Affiliation:
Department of Microbiology, University College Cork, Cork, Ireland
William Meaney
Affiliation:
Dairy Production Research Centre, Teagasc, Moorepark, Fermoy, Co.Cork, Ireland
*
*For correspondence; e-mail [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

A treatment containing a live food-grade organism, Lactococcus lactis DPC3147, was compared with conventional antibiotic therapy for its potential to treat bovine chronic subclinical or clinical mastitis in two separate field trials. Effects on disease symptoms and bacteriology were monitored in response to infusion with the culture in each trial. In the first trial, the live culture treatment was compared with an intramammary antibiotic (n=11 quarters for each treatment). Results from this small trial demonstrated that the live culture had potential to be as effective at eliminating chronic subclinical infections as an antibiotic treatment. By day 12, 7 of the 11 quarters treated with the live culture were pathogen-free compared with 5 of the 11 antibiotic-treated infected quarters. Somatic cell counts (SCC) remained relatively unchanged regardless of treatment: average log SCC pre- and post-treatment in the lactococci-treated group were 6·33±0·41 (day 0) and 6·27±0·43 cells/ml (day 12) and average log SCC pre- and post-treatment in the antibiotic-treated group were 6·34±0·37 and 6·22±0·46 cells/ml on day 0 and on day 12, respectively. In the second trial, the live culture was compared with an intramammary antibiotic for the treatment of naturally occurring clinical mastitis cases (n=25 quarters for each treatment). Following a 14-d experimental period, similar bacteriological responses were observed in 7 out of 25 live culture treated quarters and 9 out of 25 antibiotic-treated quarters. Additionally, 15 of 25 cases treated with the culture and 18 of 25 cases treated with the antibiotic did not exhibit clinical signs of the disease following treatment. The results of these trials suggest that live culture treatment with Lc. lactis DPC3147 may be as efficacious as common antibiotic treatments in some instances.

Keywords

LiveLactococcustreatmentmastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 3 , August 2008 , pp. 365 - 373
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alluwaimi, AM 2004 The cytokines of the bovine mammary gland: prospects for diagnosis and therapy. Research in Veterinary Science 77 211–222CrossRefGoogle ScholarPubMed
Almeida, RA, Batcha, T & Oliver, SP 2005 Persistence of Streptococcus uberis in bovine mammary epithelial cells. In: Proceedings of the 4th IDF International Mastitis Conference, Maastricht, The Netherlands pp. 13–142CrossRefGoogle Scholar
Barkema, HW, Schukken, YH & Zadoks, RN 2006 Invited Review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. Journal of Dairy Science 89 1877–1895CrossRefGoogle ScholarPubMed
Barrett, DJ 2004 Thesis: A Study of Mastitis in Irish Dairy Herds. University College Dublin, Ireland pp. 63–97Google Scholar
Berry, DP & Meaney, WJ 2006 Interdependence and distribution of subclinical mastitis and intramammary infection among udder quarters in dairy cattle. Preventative Veterinary Medicine 75 81–91CrossRefGoogle ScholarPubMed
Cao, LT, Wu, JQ, Xie, F, Hu, SH & Mo, Y 2007 Efficacy of nisin in the treatment of clinical mastitis in lactating dairy cows. Journal of Dairy Science 90 3980–3985CrossRefGoogle ScholarPubMed
Corlett, NJ, Peters, RR, Paape, MJ & Schultze, WD 1984 Effect of intramammary device on new infection rate, milk yield and milk somatic cell counts in Maryland dairy herds. Journal of Dairy Science 67 2571–2579CrossRefGoogle ScholarPubMed
Costello, S 2004 Consultants guide to economics of mastitis. http://www.das.psu.edu/dairynutrition/documents/mastecon.pdf. Accessed July 2006Google Scholar
Crispie, F, Twomey, D, Flynn, J, Hill, C, Ross, P & Meaney, W 2005 The lantibiotic lacticin produced in a milk-based medium improves the efficacy of a bismuth-based teat seal in cattle deliberately infected with Staphylococcus aureus. Journal of Dairy Research 72 159–67CrossRefGoogle Scholar
Crispie, F, Alonso-Gomez, M, O'Loughlin, C, Klostermann, K, Flynn, J, Arkins, S, Meaney, W, Ross, RP & Hill, C 2008 Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live lactococci on the mammary immune response. Journal of Dairy Research In pressCrossRefGoogle ScholarPubMed
Greene, WA, Gano, AM, Smith, KL, Hogan, JS & Todhunter, DA 1991 Comparison of probiotic and antibiotic therapy of cattle with elevated somatic cell count. Journal of Dairy Science 74 2976–2981CrossRefGoogle Scholar
Guterbock, WM, Van Eenennaam, AL, Anderson, RJ, Gardener, IA, Cullor, JS & Holmberg, CA 1993 Efficacy of intramammary antibiotic therapy for treatment of clinical mastitis caused by environmental pathogens. Journal of Dairy Science 76 3437–3444CrossRefGoogle ScholarPubMed
International Dairy Federation 1981 In: Laboratory Methods for Use in Mastitis Work, IDF Bulletin No. 132 International Dairy Federation, Brussels, Belgium pp. 1–27Google Scholar
Knight, CH, Fitzpatrick, JL, Logue, DN & Platt, DJ 2000 Efficacy of two non-antibiotic therapies, oxytocin and topical liniment, against bovine staphylococcal mastitis. Veterinary Record 146 311–316CrossRefGoogle ScholarPubMed
Kaneene, JB & Miller, R 1992 Description and evaluation of the influence of veterinary presence on the use of antibiotics and sulfonamides in dairy herds. Journal of the American Veterinary Medical Association 201 68–76CrossRefGoogle ScholarPubMed
Kivaria, FM, Noordhuizen, JP & Nielen, M 2007 Interpretation of California mastitis test scores using Staphylococcus aureus culture results for screening of subclinical mastitis in low yielding smallholder dairy cows in the Dar es Salaam region of Tanzania. Preventative Veterinary Medicine 78 274–285CrossRefGoogle Scholar
Klaas, IC, Wessels, U, Rothfuss, H, Tenhagen, BA, Heuwieser, W & Schallenberger, E 2004 Factors affecting reproductive performance in German Holstein-Friesian cows with a special focus on post-partum mastitis. Livestock Production Science 86 233–238CrossRefGoogle Scholar
Lees, P 1991 New insights into the pathogenesis of mastitis: General aspects of inflammation. Flemish Veterinary Journal 62 43–54Google Scholar
Nickerson, SC, Owens, WE, Tomita, GM & Widel, PW 1999 Vaccinating dairy heifers with a Staphylococcus aureus bacterin reduces mastitis at calving. Veterinary Clinics of North America: The Large Animal Practice 20 16–28Google Scholar
Oldham, ER & Daley, MJ 1991 Lysostaphin: use of a recombinant bactericidal enzyme as a mastitis therapy. Journal of Dairy Science 74 4175–4182CrossRefGoogle Scholar
Owens, WE, Ray, CH, Watts, JL & Yancey, RJ 1997 Comparison of success of antibiotic therapy during lactation and results of antimicrobial susceptibility tests for bovine mastitis. Journal of Dairy Science 80 313–317CrossRefGoogle ScholarPubMed
Reinhold, P, Schulz, J, Beuche, W & Jaekel, L 1986 The treatment of acute bovine mastitis: therapy using glucose solutions. Archive Experimenteller Veterinär Medizin 40 627–638Google ScholarPubMed
Roberson, JR, Warnick, LD & Moore, G 2004 Mild to moderate clinical mastitis: efficacy of intramammary amoxicillin, frequent milk-out, a combined intramammary amoxicillin and frequent milk-out treatment versus no treatment. Journal of Dairy Science 87 583–592CrossRefGoogle ScholarPubMed
Ryan, MP, Meaney, WJ, Ross, RP & Hill, C 1998 Evaluation of lacticin 3147 and a teat seal containing this bacteriocin for inhibition of mastitis pathogens. Applied and Environmental Microbiology 64 2287–2290CrossRefGoogle Scholar
Ryan, MP, Flynn, J, Hill, C, Ross, RP & Meaney, WJ 1999a The natural food grade inhibitor, lacticin 3147 reduced the incidence of mastitis after experimental challenge with Streptococcus dysgalactiae in nonlactating dairy cows. Journal of Dairy Science 82 2625–2631CrossRefGoogle ScholarPubMed
Ryan, MP, Jack, RW, Josten, M, Sahl, HG, Jung, G, Ross, RP & Hill, C 1999b Extensive post-translational modification, including serine to d-alanine conversion, in the two component lantibiotic lacticin 3147. Journal of Biological Chemistry 274 37544–37550CrossRefGoogle ScholarPubMed
Sandgren, CH, Waller, KP & Emanuelson, U 2008 Therapeutic effects of systemic or intramammary antimicrobial treatment of bovine subclinical mastitis during lactation. Veterinary Journal 175 108–117CrossRefGoogle ScholarPubMed
Sandholm, M, Honkanen-Buzalski, T, Kaartinen, L & Pyörälä, S 1995 The Bovine Udder and Mastitis. ISBN 951-834-047-1. University of Helsinki, Faculty of Veterinary MedicineGoogle Scholar
Sears, PM, Smith, BS, English, PB, Herer, PS & Gonzalez, RN 1990 Shedding pattern of Staphylococcus aureus from bovine intramammary infections. Journal of Dairy Science 73 2785–2789CrossRefGoogle ScholarPubMed
Simpson, PJ, Stanton, C, Fitzgerald, GF & Ross, RP 2002 Genomic diversity within the genus Pediococcus as revealed by randomly amplified polymorphic DNA PCR and pulsed-field gel electrophoresis. Applied and Environmental Microbiology 68 765–771CrossRefGoogle ScholarPubMed
Silanikove, N, Iscovich, J & Leitner, G 2005 Therapeutic treatment with casein hydrolysate eradicates effectively bacterial infection in treated mammary quarters in cows. In: Proceedings of the 4th IDF International Mastitis Conference, Maastricht, The Netherlands pp. 327–332CrossRefGoogle Scholar
Swinkels, JM, Hogeveen, H & Zadoks, RN 2005 A partial budget model to estimate benefits of lactational treatment of subclinical Staphylococcus aureus mastitis. Journal of Dairy Science 88 4273–4287CrossRefGoogle ScholarPubMed
Twomey, DP, Wheelock, AI, Flynn, J, Meaney, WJ, Hill, C & Ross, RP 2000 Protection against Staphylococcus aureus mastitis in dairy cows using a bismuth-based teat seal containing the bacteriocin, lacticin 3147. Journal of Dairy Science 83 1981–1988CrossRefGoogle ScholarPubMed
Varshney, J P & Naresh, R 2004 Evaluation of a homeopathic complex in the clinical management of udder diseases of riverine buffaloes. Homeopathy 93 17–20CrossRefGoogle ScholarPubMed
Wilson, DJ, Gonzalez, RN, Case, KL, Garrison, LL & Groehn, YT 1999 Comparison of seven antibiotic treatments with no treatment for bacteriological efficacy against bovine mastitis pathogens. Journal of Dairy Science 82 1640–1670CrossRefGoogle ScholarPubMed
Wu, J, Hu, S & Cao, L 2007 Therapeutic effect of nisin Z on subclinical mastitis in lactating cows. Antimicrobial Agents and Chemotherapy 51 3131–3135CrossRefGoogle ScholarPubMed
Zadoks, RN, Gillespie, BE, Barkema, HW, Sampimon, OC, Oliver, SP & Schukken, YH 2003 Clinical, epidemiological and molecular characteristics of Streptococcus uberis infections in dairy herds. Epidemiology and Infection 130 335–349CrossRefGoogle ScholarPubMed