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Interrelationships between acetylation and the disposal of acetyl groups in the livers of dairy cows

Published online by Cambridge University Press:  01 June 2009

Alan M. Snoswell ,
Nicholas D. Costa ,
Jock G. Mclean ,
G.david Baird ,
Michael A. Lomax  and
Herbert W. Symonds
Alan M. Snoswell
Affiliation:
Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia
Nicholas D. Costa
Affiliation:
Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia
Jock G. Mclean
Affiliation:
Department of Veterinary Pre-Clinical Science, University of Melbourne, Parkville, Victoria, Australia
G.david Baird
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 0NN
Michael A. Lomax
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 0NN
Herbert W. Symonds
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 0NN
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Summary

Possible mechanisms by which the degree of acetylation of hepatic CoA might be regulated were examined in lactating and in non-lactating dairy cows. This involved the measurement of the hepatic steady-state concentrations of free CoA, acetyl-CoA, free carnitine, acetylcarnitine and total acid-soluble carnitine in freezeclamped biopsy samples, the measurement of the hepatic release of acetate from acetylcarnitine in vitro, and the measurement of the rate of hepatic output of acetate and ketone bodies in vivo.

The hepatic ratio of [free CoA]/[acetyl-CoA] was 0·11 in lactating cows and 0·59 in non-lactating cows. There was a significant rise in the hepatic concentration of acetyl-CoA and a significant fall in that of total acid-soluble carnitine in the lactating cows as compared with the non-lactating cows.

There was a net in vivo output of acetate and of ketone bodies from the livers of both lactating and non-lactating cows. The rates of output amounted to 7·77 and 2·00 mmole/min for acetate and ketone bodies respectively in the lactating cows, and 4·95 and 2·14 mmole/min in the non-lactating cows.

The rate of enzymic release of acetate from acetylcarnitine in vitro amounted to 0·93 and 0·34 μmole min−1 (g wet wt)−1 at 37 °C for liver homogenates derived from lactating and non-lactating cows respectively. The activity observed in the case of the lactating cows was sufficient to account for the rate of hepatic acetate output observed in vivo.

Type
Original Articles
Information
Journal of Dairy Research , Volume 45 , Issue 3 , October 1978 , pp. 331 - 338
Copyright
Copyright © Proprietors of Journal of Dairy Research 1978

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References

REFERENCES

Allred, J. B. & Guy, D. G. (1969). Analytical Biochemistry 29, 293.CrossRefGoogle Scholar
Baird, G. D. (1977). Biochemical Society Transactions 5, 819.CrossRefGoogle Scholar
Baird, G. D. & Heitzman, R. J. (1970). Biochemical Journal 116, 865.CrossRefGoogle Scholar
Baird, G. D., Heitzman, R. J. & Snoswell, A. M. (1972). European Journal of Biochemistry 29, 104.CrossRefGoogle Scholar
Baird, G. D., Symonds, H. W. & Ash, R. (1975). Journal of Agricultural Science 85, 281.CrossRefGoogle Scholar
Costa, N. D. & Snoswell, A. M. (1975 a). Biochemical Journal 152, 161.CrossRefGoogle Scholar
Costa, N. D. & Snoswell, A. M. (1975 b). Biochemical Journal 152, 167.CrossRefGoogle Scholar
Costa, N. D., McIntosh, G. H. & Snoswell, A. M. (1976). Australian Journal of Biological Sciences 29, 33.CrossRefGoogle Scholar
Fritz, I. B., Schultz, S. K. & Srere, P. A. (1963). Journal of Biological Chemistry 238, 2509.CrossRefGoogle Scholar
Harvey, R. B. & Brothers, A. J. (1962). Annals of the New York Academy of Sciences 102, 46.CrossRefGoogle Scholar
Katz, M. L. & Bergman, E. N. (1969 a). American Journal of Physiology 216, 946.CrossRefGoogle Scholar
Katz, M. L. & Bergman, E. N. (1969 b). American Journal of Physiology 216, 953.CrossRefGoogle Scholar
Knowles, S. E., Jarrett, I. G., Filsell, O. H. & Ballard, F. J. (1974). Biochemical Journal 142, 401.CrossRefGoogle Scholar
Marquis, N. R. & Fritz, I. B. (1964). Journal of Lipid Research 5, 184.CrossRefGoogle Scholar
Mellanby, J. & Williamson, D. H. (1974). In Methods of Enzymatic Analysis, 2nd edn, vol. 4, p. 1840. (Ed. Bergmeyer, H. U.). New York: Academic Press.CrossRefGoogle Scholar
Pearson, D. J. & Tubbs, P. K. (1964). Nature, London 202, 91.CrossRefGoogle Scholar
Pearson, D. J. & Tubbs, P. K. (1967). Biochemical Journal 105, 953.CrossRefGoogle Scholar
Quraishi, S. & Cook, R. M. (1972). Journal of Agricultural and Food Chemistry 20, 91.CrossRefGoogle Scholar
Seufert, C. D., Graf, M., Janson, G., Kuhn, A. & Söling, H. D. (1974). Biochemical and Biophysical Research Communications 57, 901.CrossRefGoogle Scholar
Snoswell, A. M. & Henderson, G. D. (1970). Biochemical Journal 119, 59.CrossRefGoogle Scholar
Snoswell, A. M. & Koundakjian, P. P. (1972). Biochemical Journal 127, 133.CrossRefGoogle Scholar
Snoswell, A. M. & Tubbs, P. K. (1978). Biochemical Journal 171, 299.CrossRefGoogle Scholar
Symonds, H. W. & Baird, G. D. (1973). Research in Veterinary Science 14, 267.CrossRefGoogle Scholar
Williamson, D. H. & Mellanby, J. (1974). In Methods of Enzymatic Analysis, 2nd edn, vol. 4, p. 1836. (Ed. Bergmeyer, H. U.). New York: Academic Press.CrossRefGoogle Scholar
Williamson, D. H., Veloso, D., Ellington, E. V. & Krebs, H. A. (1969). Biochemical Journal 114, 575.CrossRefGoogle Scholar