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Interferences on microbial inhibitor tests related to ivermectin treatment in lactating dairy goats

Published online by Cambridge University Press:  07 September 2016

Tamara Romero ,
Vicente Javier Moya ,
Nemesio Fernández ,
Rafael Althaus ,
Wim Reybroeck  and
María Pilar Molina
Tamara Romero*
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
Vicente Javier Moya
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
Nemesio Fernández
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
Rafael Althaus
Affiliation:
Cátedra de Biofísica, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral. R.P.L., Kreder. 3080. Esperanza, Argentina
Wim Reybroeck
Affiliation:
Institute for Agricultural and Fisheries Research, Technology and Food Science Unit (ILVO-T&V), Brusselsesteenweg 370, 9090 Melle, Belgium
María Pilar Molina
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
*
*For correspondence; e-mail: [email protected]
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Abstract

This Research Communication reports interferences related to the administration of ivermectin in lactating dairy goats on the response of microbial tests for screening antibiotics in milk. Twenty-eight Murciano-Granadina goats, naturally infested with Sarcoptes scabiei var. caprae, were treated with a subcutaneous injection of ivermectin (200 µg/kg b.w.). To prevent re-infestation, a second dose was applied 7 d later. Individual milk samples were collected, daily, up to 15 d post-treatment. Milk samples were analysed by microbial inhibitor tests (BRT MRL, Delvotest SP-NT MCS and Eclipse 100) and ivermectin residues were quantified by HPLC. A large number of positive results were obtained for all microbial tests, especially on the first day after treatment (BRT MRL = 46·4%; Delvotest SP-NT MCS = 14·3%; and Eclipse 100 = 17·8%). However, the highest concentration of drug residues in milk (24·3 ng/ml) was detected on the tenth day after treatment, when positive outcomes were relatively lower (BRT MRL = 17·8%; Delvotest SP-NT MCS = 10·7%; and Eclipse 100 = 7·4%). Results herein suggest that factors related to the ivermectin treatment other than drug residues in milk, or alterations produced by the parasitic disease itself affecting the immune response of animals, could be the cause of false-positive results in microbial tests. It can be concluded that the application of ivermectin in dairy goats infested with sarcoptes mange during lactation produces persistent drug residues in milk, and could also cause false-positive results in microbial inhibitor tests for screening antibiotics.

Keywords

Goat milkivermectinmicrobial inhibitor tests
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 341 - 344
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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References

Andrew, SM 2001 Effect of composition of colostrum and transition milk from Holstein heifers on specificity rates of antibiotic residue tests. Journal Dairy Science 84 100–106 CrossRefGoogle ScholarPubMed
Althaus, RL, Torres, A, Peris, C, Beltrán, MC, Fernández, N & Molina, MP 2003 Accuracy of BRT and Delvotest microbial inhibitor tests as affected by composition of ewe's milk. Journal Food Protection 66 473–478 Google Scholar
Alvineire, M, Sutra, JF & Galtier, P 1993 Ivermectin in goat plasma and milk after subcutaneous injection. Annals of Veterinary Research 24 417–421 Google Scholar
Beltrán, MC, Berruga, MI, Molina, A, Althaus, RL & Molina, MP 2015 Performance of the current microbial tests for screening antibiotic in sheep and goat milk. International Dairy Journal 41 13–15 CrossRefGoogle Scholar
Commission Regulation (EU) No 37/ 2010 On pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. Official Journal of the European Union 15 1–72 Google Scholar
EMA, European Medicine Agency 2014 European Public MRL Assessment Report. Ivermectin (all mammalian food producing species). EMA/CVMP/294840/2014. http://www.ema.europa.eu/docs/en_GB/document_library/Maximum_Residue_Limits_-_Report/2014/05/WC500167329.pdf Google Scholar
Fernanda, A, Imperiale, F, Margarita, R & Lanusse, E 2004 Milk excretion of ivermectin and moxidectin in dairy sheep cheese elaboration. Food Chemistry 52 6205–6211 Google Scholar
Imperiale, F, Lifschitz, A, Sallovitz, J, Virkel, G & Lanusse, C 2004 Comparative depletion of ivermectin and moxidectin milk residues in dairy sheep after oral and subcutaneous administration. Journal Dairy Research 71 427–433 Google Scholar
Lastras, ME, Pastor, J, Marco, I, Ruiz, M, Viñas, L & Lavin, S 2000 Effects of sarcoptic mange on serum proteins and immunoglobulin G levels in chamois (Rupicabra pyrenaica) and Spanish ibex (Capra pyrenaica). Veterinary Parasitology 88 131–319 Google Scholar
McKellar, QA & Benchaoui, HA 1996 Avermectins and milbemycins. Journal of Veterinary Pharmacology and Therapeutics 19 331–351 Google Scholar
Mullins, JS, Arlian, LG & Morgan, MS 2009 Extracts of Sarcoptes scabiei De Geer down modulate secretion of IL-8 by skin keratinocytes and fibroblasts and of GM-CSF by fibroblasts in presence of proinflamatory cytokines. Journal of Medical Entomology 46 845–851 CrossRefGoogle Scholar
Rahman, M, Lecchi, C, Fraquelli, C, Sartorelli, P & Ceciliani, F 2010 Acute phase protein response in Alpine ibex with sarcoptic mange. Veterinary Parasitology 168 293–298 CrossRefGoogle ScholarPubMed
Regulation (EC) No 853/ 2004 of the European Parliament and of the Council. Laying down specific hygiene rules for on the hygiene of foodstuffs. Official Journal of the European Union 139 55–205 Google Scholar
Romero, T, Beltrán, MC, Althaus, RL & Molina, MP 2014 Detection of antibiotics in goat's milk: effect of detergents on the response of microbial inhibitor tests. Journal Dairy Research 81 372–377 CrossRefGoogle ScholarPubMed
Romero, T, Beltrán, MC, Reybroeck, W & Molina, MP 2015 Effect in vitro of antiparasitic drugs on microbial inhibitor test responses for screening antibiotic residues in goat's milk. Journal Food Protection 78 1756–1759 Google Scholar
Sajid, MS, Iqbal, Z, Muhammad, G, Sandhu, MA & Khan, MN 2007 Effect of ivermectin on the cellular and humoral immune responses of rabbits. Life Sciences 80 1966–1970 CrossRefGoogle ScholarPubMed
Stankiewicz, M, Cabaj, W, Jonas, WE, Moore, LG, Millar, K & Chie, W 1995 Influence of ivermectin on cellular and humoral immune responses of lambs. Veterinary Immunology and Immunopathology 44 347–358 Google Scholar
Ujjwal, KD & Dey, S 2010 Evaluation of organ function and oxidant/antioxidant status in goats with sarcoptic mange. Tropical Animal Health and Production 42 1663–1668 Google Scholar
Walton, SF & Currie, BJ 2007 Problems in diagnosing scabies, a global disease in human and animal population. Clinical Microbiology Reviews 20 268–279 CrossRefGoogle Scholar