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Innovative application of postbiotics, parabiotics and encapsulated Lactobacillus plantarum RM1 and Lactobacillus paracasei KC39 for detoxification of aflatoxin M1 in milk powder

Published online by Cambridge University Press:  23 December 2021

Karima Mogahed Fahim ,
Ahmed Noah Badr ,
Mohamed Gamal Shehata ,
Eman Ibrahim Hassanen  and
Lamiaa Ibrahim Ahmed
Karima Mogahed Fahim*
Affiliation:
Food Hygiene and Control Department, Faculty of Veterinary Medicine, Cairo University, Giza12211, Egypt
Ahmed Noah Badr
Affiliation:
Department of Food Toxicology and Contaminants, National Research Centre, Dokki, 12622Cairo, Egypt
Mohamed Gamal Shehata
Affiliation:
Department of Food Technology, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Application, Alexandria, Egypt
Eman Ibrahim Hassanen
Affiliation:
Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza12211, Egypt
Lamiaa Ibrahim Ahmed
Affiliation:
Food Hygiene and Control Department, Faculty of Veterinary Medicine, Cairo University, Giza12211, Egypt
*
Author for correspondence: Karima Mogahed Fahim, Email: [email protected]
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Abstract

This study aimed to evaluate aflatoxin M1 (AFM1) level in milk powder and infant milk formulae, in addition to applying innovative methods for AFM1 & AFB1 detoxification. Fifty random samples of milk powder and infant formulae (25 of each) were collected from the Egyptian markets for assessing AFM1 level using ELISA technique. Bioactive components comprising cell free supernatants (postbiotic), acid-dead cells (parabiotic) and the encapsulated-cells of Lactobacillus plantarum RM1 and Lactobacillus paracasei KC39 were evaluated for their antifungal activity against toxigenic mold strains and their impact on AFB1 and AFM1 reduction in reconstituted milk powder. AFM1 concentration in unpacked milk powder was higher than that of packed samples and infant formulae, although these differences were not significant (P > 0.05). About 96.0, 29.4 and 25.0% of the tested infant formulae, unpacked, and packed milk powder were unacceptable in terms of the AFM1 limit defined by Egyptian and European standards, while all samples were in accordance with the USA/FDA standard. All tested mycotoxigenic strains were sensitive to the different treatments of the probiotics with the highest sensitivity regarding Fusarium strain with L. paracasei KC39 compared to other genera. The degradation ratios of AFM1 using the bioactives of the L. paracasei KC39 were higher than that of L. plantarum RM1 bioactives. Additionally, KC39 parabiotic manifested the best AFB1 reduction (60.56%). In conclusion, the positive and highly significant relationship (P < 0.05) between these effective biocompounds mirrors their major detoxification role which gives a safe solution for AFs contamination issues in milk and milk products.

Keywords

Aflatoxins detoxificationbioactive componentsinfants formulaemilk powderprobiotic
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 4 , November 2021 , pp. 429 - 435
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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