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Immunochemical, chromatographic, and milk-clotting activity measurements for quantification of milk-clotting enzymes in bovine rennets

Published online by Cambridge University Press:  01 June 2009

G. A. L. Rothe ,
N. H. Axelsen ,
P. Jøhnk  and
B. Foltmann
G. A. L. Rothe
Affiliation:
Research Department of Chr. Hansen's Laboratory, Sankt Annœ Plads 3, DK-1250 Copenhagen K, Denmark
N. H. Axelsen
Affiliation:
Research Department of Chr. Hansen's Laboratory, Sankt Annœ Plads 3, DK-1250 Copenhagen K, Denmark
P. Jøhnk
Affiliation:
Research Department of Chr. Hansen's Laboratory, Sankt Annœ Plads 3, DK-1250 Copenhagen K, Denmark
B. Foltmann
Affiliation:
Research Department of Chr. Hansen's Laboratory, Sankt Annœ Plads 3, DK-1250 Copenhagen K, Denmark
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Summary

Monospecific rabbit antibodies were produced against bovine chymosin (rennin) and bovine pepsin A and used for quantitative measurements of these enzymes by rocket immunoelectrophoresis. Bovine pepsin B was identified and measured quantitatively in tandem crossed immunoelectrophoresis, using a polyspecific antibody preparation. Chromatographically purified chymosin B and bovine pepsins A and B were used as standards. The contents of chymosin and bovine pepsin A were also determined by 2 forms of DEAE chromatography, and by an assay utilizing the ratio of milk-clotting activity at pH 6·0 and 6·5. Results obtained by measurements on 3 bovine rennets (calf rennet, adult bovine rennet, and a mixture of such types) by the different methods of analysis were in good agreement. The advantages of the immunochemical methods are direct and simultaneous identification and quantitation of enzymes.

Type
Research Article
Information
Journal of Dairy Research , Volume 43 , Issue 1 , February 1976 , pp. 85 - 95
Copyright
Copyright © Proprietors of Journal of Dairy Research 1976

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