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Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil

Published online by Cambridge University Press:  10 August 2021

Thais Cristina de Assis Oliveira ,
Maria Aparecida Vasconcelos Paiva Brito ,
Marcia Giambiagi-de Marval ,
Nívea Maria Vicentini  and
Carla Christine Lange
Thais Cristina de Assis Oliveira
Affiliation:
Graduate Program in Science and Technology of Milk and Derivatives, Federal University of Juiz de Fora, Juiz de Fora, Brazil
Maria Aparecida Vasconcelos Paiva Brito
Affiliation:
Embrapa Dairy Cattle Research Center, Juiz de Fora, Brazil
Marcia Giambiagi-de Marval
Affiliation:
Department of Medical Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Nívea Maria Vicentini
Affiliation:
Embrapa Dairy Cattle Research Center, Juiz de Fora, Brazil
Carla Christine Lange*
Affiliation:
Embrapa Dairy Cattle Research Center, Juiz de Fora, Brazil
*
Author for correspondence: Carla Christine Lange, Email: [email protected]
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Abstract

In this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.

Keywords

Bovine intramammary infectiongram-negative bacteriamass spectrometrystaphylococcistreptococci
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 302 - 306
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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