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Identification of an immune modulation locus utilising a bovine mammary gland infection challenge model

Published online by Cambridge University Press:  22 May 2018

Mathew D Littlejohn ,
Sally-Anne Turner ,
Caroline G Walker ,
Sarah D Berry ,
Kathryn Tiplady ,
Ric G Sherlock ,
Greg Sutherland ,
Simon Swift ,
Dorian Garrick  and
S Jane Lacy-Hulbert
...Show all authors
Mathew D Littlejohn
Affiliation:
LIC, Hamilton, New Zealand
Sally-Anne Turner*
Affiliation:
DairyNZ, Hamilton, New Zealand
Caroline G Walker
Affiliation:
DairyNZ, Hamilton, New Zealand
Sarah D Berry
Affiliation:
Growing up in New Zealand, University of Auckland, Auckland, New Zealand
Kathryn Tiplady
Affiliation:
LIC, Hamilton, New Zealand
Ric G Sherlock
Affiliation:
LIC, Hamilton, New Zealand
Greg Sutherland
Affiliation:
School of Medical Sciences, University of Sydney, Sydney, Australia
Simon Swift
Affiliation:
School of Medical Sciences, University of Auckland, Auckland, New Zealand
Dorian Garrick
Affiliation:
Massey University, Palmerston North, New Zealand
S Jane Lacy-Hulbert
Affiliation:
DairyNZ, Hamilton, New Zealand
Scott McDougall
Affiliation:
Cognsoco, Anexa Animal Health, Morrinsville, New Zealand
Richard J Spelman
Affiliation:
LIC, Hamilton, New Zealand
Russell G Snell
Affiliation:
School of Biological Sciences, University of Auckland, Auckland, New Zealand
J Eric Hillerton
Affiliation:
DairyNZ, Hamilton, New Zealand
*
*For correspondence; e-mail: [email protected]
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Abstract

Inflammation of the mammary gland following bacterial infection, commonly known as mastitis, affects all mammalian species. Although the aetiology and epidemiology of mastitis in the dairy cow are well described, the genetic factors mediating resistance to mammary gland infection are not well known, due in part to the difficulty in obtaining robust phenotypic information from sufficiently large numbers of individuals. To address this problem, an experimental mammary gland infection experiment was undertaken, using a Friesian-Jersey cross breed F2 herd. A total of 604 animals received an intramammary infusion of Streptococcus uberis in one gland, and the clinical response over 13 milkings was used for linkage mapping and genome-wide association analysis. A quantitative trait locus (QTL) was detected on bovine chromosome 11 for clinical mastitis status using micro-satellite and Affymetrix 10 K SNP markers, and then exome and genome sequence data used from the six F1 sires of the experimental animals to examine this region in more detail. A total of 485 sequence variants were typed in the QTL interval, and association mapping using these and an additional 37 986 genome-wide markers from the Illumina SNP50 bovine SNP panel revealed association with markers encompassing the interleukin-1 gene cluster locus. This study highlights a region on bovine chromosome 11, consistent with earlier studies, as conferring resistance to experimentally induced mammary gland infection, and newly prioritises the IL1 gene cluster for further analysis in genetic resistance to mastitis.

Keywords

Mastitisimmune modulationgenetics
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 185 - 192
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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