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Hormone-dependent expression of the ovine β-lactoglobulin gene

Published online by Cambridge University Press:  01 June 2009

Rebecca Osborne ,
Michael Howell ,
A. John Clark  and
Kevin R. Nicholas
Rebecca Osborne
Affiliation:
CSIRO, Division of Wildlife and Ecology, PO Box 84, Lyneham, ACT 2602, Australia Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT 2601, Australia
Michael Howell
Affiliation:
CSIRO, Division of Wildlife and Ecology, PO Box 84, Lyneham, ACT 2602, Australia Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT 2601, Australia
A. John Clark
Affiliation:
CSIRO, Division of Wildlife and Ecology, PO Box 84, Lyneham, ACT 2602, Australia †Roslin Institute, Edinburgh Research Station, Roslin, Midlothian EH25 9PS, UK
Kevin R. Nicholas
Affiliation:
CSIRO, Division of Wildlife and Ecology, PO Box 84, Lyneham, ACT 2602, Australia
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Summary

The minimal hormonal requirements for inducing the ovine β-lactoglobulin gene have been investigated using mammary gland explants from ewes in the first half of pregnancy. Quantification of β-lactoglobulin mRNA showed that a combination of insulin, Cortisol and prolactin was required to stimulate the expression of the gene and that this response could not be enhanced by the addition of oestrogen and thyroid hormone to the culture medium. Explants cultured in the presence of insulin, Cortisol and prolactin also demonstrated the capacity to synthesize the protein. Progesterone did not inhibit the induction of the gene, which is consistent with the increase in β-lactoglobulin mRNA observed in vivo in the mammary gland during the final 2 months of pregnancy when the circulating level of progesterone is elevated.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 2 , May 1995 , pp. 321 - 329
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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References

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