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Histological examination of non-lactating bovine udders inoculated with Lactobacillus perolens CRL 1724

Published online by Cambridge University Press:  09 November 2012

Ignacio D Frola ,
Matías S Pellegrino ,
Gabriel Magnano ,
José A Giraudo ,
María C Espeche ,
María EF Nader-Macias  and
Cristina I Bogni
Ignacio D Frola
Affiliation:
Department of Microbiology and Immunology, Faculty of Cs. Ex. Fco-Qcas y Naturales, University of Río Cuarto, Ruta 8 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
Matías S Pellegrino
Affiliation:
Department of Microbiology and Immunology, Faculty of Cs. Ex. Fco-Qcas y Naturales, University of Río Cuarto, Ruta 8 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
Gabriel Magnano
Affiliation:
Department of Animal Pathology, Faculty of Agronomy and Veterinary, University of Río Cuarto, Ruta 8 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
José A Giraudo
Affiliation:
Department of Animal Pathology, Faculty of Agronomy and Veterinary, University of Río Cuarto, Ruta 8 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
María C Espeche
Affiliation:
Departament of Preventive Microbiology, CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Cientifícas y Técnicas de Argentina), Chacabuco 145, 4000 San Miguel de Tucumán, Argentina
María EF Nader-Macias
Affiliation:
Departament of Preventive Microbiology, CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Cientifícas y Técnicas de Argentina), Chacabuco 145, 4000 San Miguel de Tucumán, Argentina
Cristina I Bogni*
Affiliation:
Department of Microbiology and Immunology, Faculty of Cs. Ex. Fco-Qcas y Naturales, University of Río Cuarto, Ruta 8 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
*
*For correspondence; e-mail: [email protected]
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Abstract

The effect of intramammary inoculation of Lactobacillus perolens CRL 1724 on bovine udders at drying off was evaluated through histological examination of the canal and cistern tissues. The persistence of the strain in the udder 7 d post inoculation was also determined. Lb. perolens CRL 1724 was recovered from all mammary quarters and no clinical signs or teat damage were observed after inoculation of 106 cfu/ml. The udders showed a normal structural aspect and there were no modifications of the milk appearance. Lb. perolens CRL 1724 cells were evidenced on the surface of the epithelial cells of the cistern without causing any morphological modifications or cell alterations. Lb. perolens CRL 1724 produces a mild inflammatory reaction, characterized by recruitment of neutrophils to the epithelial zone and a slight hyperaemia into blood vessels. This preliminary study provides important information for further studies directed towards the inclusion of Lb. perolens CRL 1724 in the design of probiotic products for preventing bovine mastitis in non-lactating dairy cows.

Keywords

Bovine mastitisLactobacillus perolens CRL 1724adherencenon-lactating dairy cows
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 1 , February 2013 , pp. 28 - 35
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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