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Histochemical localization and possible antibacterial role of xanthine oxidase in the bovine mammary gland

Published online by Cambridge University Press:  01 June 2009

Robert A. Collins ,
Keith R. Parsons ,
Terry R. Field  and
A. John Bramley
Robert A. Collins
Affiliation:
AFRC Institute for Animal Disease Research, Compton Laboratory, Compton, Newbury, RG16 0NN, UK
Keith R. Parsons
Affiliation:
AFRC Institute for Animal Disease Research, Compton Laboratory, Compton, Newbury, RG16 0NN, UK
Terry R. Field
Affiliation:
AFRC Institute for Animal Disease Research, Compton Laboratory, Compton, Newbury, RG16 0NN, UK
A. John Bramley
Affiliation:
AFRC Institute for Animal Disease Research, Compton Laboratory, Compton, Newbury, RG16 0NN, UK
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Summary

Xanthine oxidase (XO) was demonstrated to be present in the teat canal and secretory tissue of the bovine mammary gland by histochemical techniques. Homogenates of these tissues were able to replace XO in an antibacterial assay with Streptococcus uberis. The action of XO on its substrate hypoxanthine was shown to provide an essential component for anti-streptococcal activity mediated by lactoperoxidase. A mechanism is proposed whereby the interaction of XO, lactoperoxidase and thiocyanate may provide antibacterial activity in the teat canal.

Type
Original articles
Information
Journal of Dairy Research , Volume 55 , Issue 1 , February 1988 , pp. 25 - 32
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

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References

REFERENCES

Adams, E. W., Rickard, C. G. & Murphy, J. M. 1961 Some histological and histochemical observations on bovine teat epithelium. Cornell Veterinarian 51 124–154Google ScholarPubMed
Aune, T. M. & Thomas, E. L. 1977 Accumulation of hypothiocyanite ion during peroxidase-catalyzed oxidation of thiocyanate ion. European Journal of Biochemistry 80 209–214CrossRefGoogle ScholarPubMed
Björck, L. & Claesson, O. 1979 Xanthine oxidase as a source of hydrogen peroxide for the lactoperoxidase system in milk. Journal of Dairy Science 62 1211–1215CrossRefGoogle Scholar
Björck, L. & Claesson, O. 1980 Correlation between concentration of hypothiocyanate and antibacterial effect of the lactoperoxidase system against Escherichia coli. Journal of Dairy Science 63 919–922CrossRefGoogle Scholar
Bruder, G., Heid, H. W., Jarasch, E-D. & Mather, I. H. 1983 Immunological identification and determination of xanthine oxidase in cells and tissues. Differentiation 23 218–225CrossRefGoogle ScholarPubMed
Chandler, R. L., Lepper, A. W. D. & Wilcox, J. 1969 Ultrastructural observations on the bovine teat duct. Journal of Comparative Pathology 79 315–319CrossRefGoogle ScholarPubMed
Cousins, C. L., Higgs, T. M., Jackson, E. R., Neave, F. K. & Dodd, F. H. 1980 Susceptibility of the bovine udder to bacterial infection in the dry period. Journal of Dairy Research 47 11–18CrossRefGoogle ScholarPubMed
Hibbitt, K. G. & Benians, M. 1971 Some effects in vivo of the teat canal and effects in vitro of cationic proteins on staphylococci. Journal of General Microbiology 68 123–128CrossRefGoogle ScholarPubMed
Homan-Muller, J. W. T., Weening, R. S. & Roos, D. 1975 Production of hydrogen peroxide by phagocytosing human granulocytes. Journal of Laboratory and Clinical Medicine 85 198–207Google Scholar
Jarasch, E-D., Grund, C., Bruder, G., Heid, H. W., Keenan, T. W. & Franke, W. W. 1981 Localisation of xanthine oxidase in mammary gland epithelium and capillary endothelium. Cell 25 67–82CrossRefGoogle ScholarPubMed
Klebanoff, S. J., Clem, W. H. & Luebke, R. G. 1966 The peroxidase-thiocyanate-hydrogen peroxide antimicrobial system. Biochimica et Biophysica Acta 117 63–72CrossRefGoogle ScholarPubMed
McDonald, J. S., 1975 Radiographic method for anatomie study of the teat canal: changes between milking periods. American Journal of Veterinary Research 36 1241–1242Google Scholar
Macy, I. G., Kelly, H. J. & Sloan, R. E. 1953 The composition of milks. National Academy of Sciences—National Research Council Publication no. 254Google Scholar
Mangino, M. E. & Brunner, J. R. 1977 Isolation and partial characterization of xanthine oxidase associated with the milk fat globule membrane of cows' milk. Journal of Dairy Science 60 841–850CrossRefGoogle Scholar
Marshall, V. M. E., Cole, W. M. & Bramley, A. J. 1986 Influence of the lactoperoxidase system on susceptibility of the udder to Streptococcus uberis infection. Journal of Dairy Research 53 507–514CrossRefGoogle ScholarPubMed
Marshall, V. M. E. & Reiter, B. 1980 Comparison of the antibacterial activity of the hypothiocyanite anion towards Streptococcus lactis and Escherichia coli. Journal of General Microbiology 120 513–516Google ScholarPubMed
Nakano, K., Assenza, S. P. & Brown, P. R. 1982 Reversed-phase liquid Chromatographic investigation of UV-absorbing low-molecular-weight compounds in saliva. Journal of Chromatography 233 51–60CrossRefGoogle ScholarPubMed
Oram, J. D. & Reiter, B. 1966 The inhibition of streptococci by lactoperoxidase, thiocyanate and hydrogen peroxide. I. The effect of the inhibitory system on susceptible and resistant strains of group N streptococci. Biochemical Journal 100 373–382CrossRefGoogle ScholarPubMed
Reiter, B. 1985 Protective proteins in milk: biological significance and exploitation. International Dairy Federation Bulletin Document no. 191 1–35Google Scholar
Reiter, B. & Bramley, A. J. 1975 Defence mechanisms of the udder and their relevance to mastitis control. International Dairy Federation Bulletin Document no. 85 210–222Google Scholar
Sackler, M. L. 1966 Xanthine oxidase from liver and duodenum of the rat: histochemical localization and electrophoretic heterogeneity. Journal of Histochemistry and Cytochemistry 14 326–333CrossRefGoogle ScholarPubMed
Williams, M. R. & Bunch, K. J. 1981 Variation among cows in the ability of their blood polymorphonuclear leucocytes to kill Escherichia coli and Staphylococcus aureus. Research in Veterinary Science 30 298–302CrossRefGoogle ScholarPubMed