Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-27T06:35:15.319Z Has data issue: false hasContentIssue false
  • English
  • Français

High-throughput pH monitoring method for application in dairy fermentations

Published online by Cambridge University Press:  22 August 2018

Valery Gutsal ,
Sander Sieuwerts  and
Rodrigo Bibiloni
Valery Gutsal*
Affiliation:
Arla Innovation Centre, Agro Food Park 19, 8200 Aarhus N, Denmark
Sander Sieuwerts
Affiliation:
Arla Innovation Centre, Agro Food Park 19, 8200 Aarhus N, Denmark
Rodrigo Bibiloni
Affiliation:
Arla Innovation Centre, Agro Food Park 19, 8200 Aarhus N, Denmark
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Optimization of dairy fermentation processes often requires multiplexed pH measurements over several hours. The method developed here measures up to 90 samples simultaneously, where traditional electrode-based methods require a lot more time for handing the same number of samples. Moreover, the new method employs commonly used materials and can be used with a wider range of fluorescence readers than commercial 96-well plates with optical pH sensors. For this application, a milk-like transparent medium is developed that shows acidification properties with dairy starters that are similar to milk. Combination of this milk-like medium and 3 fluorescent indicators allow precise measurements of pH in a range of 4·0–7·0. The new method showed much higher throughput compared to the benchmark electrode systems while being as accurate, as shown by successful application for a comparison of various dairy starter cultures and for optimizing the inoculation rate.

Keywords

Dairy starter culturespH monitoringfluorescence indicatorsacidification curvemilk-like mediummulti well plate reader
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 4 , November 2018 , pp. 453 - 459
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arain, S, John, GT, Krause, C, Gerlach, J, Wolfbeis, OS & Klimant, I 2006 Characterization of microtiter plates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays. Sensors and Actuators B: Chemical 113 639–648Google Scholar
Carminati, D, Giraffa, G, Quiberoni, A, Binetti, A, Suárez, V & Reinheimer, J 2010 Biotechnology of lactic acid bacteria: Novel applications. Advances and Trends in Starter Cultures for Dairy Fermentations 177 177–192Google Scholar
John, GT, Goelling, D, Klimant, I, Schneider, H & Heinzle, E 2003 pH-sensing 96-well microtitre plates for the characterization of acid production by dairy starter cultures. Journal of Dairy Research 70 327–333Google Scholar
Kensy, F, Zang, E, Faulhammer, C, Tan, RK & Büchs, J 2009 Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates. Microbial Cell Factories 8 31–00Google Scholar
Kermis, HR, Kostov, Y, Harms, P & Rao, G 2002 Dual excitation ratiometric fluorescent pH sensor for noninvasive bioprocess monitoring: development and application. Biotechnology Progress 18 1047–1053Google Scholar
Kim, JH, Johannes, L, Goud, B, Antony, C, Lingwood, CA, Daneman, R & Grinstein, S 1998 Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release. Proceedings of the National Academy of Sciences 95 2997–3002Google Scholar
McMillan, G 1991 Understand some basic truths of pH measurement. Chemical Engineering Progress 87 30–37Google Scholar
Nedergaard, M, Desai, S & Pulsinelli, W 1990 Dicarboxy-dichlorofluorescein: a new fluorescent probe for measuring acidic intracellular pH. Analytical Biochemistry 187 109–114Google Scholar
Sipior, J, Bambot, S, Romauld, M, Carter, GM, Lakowicz, JR & Rao, G 1995 A lifetime-based optical CO2 gas sensor with blue or red excitation and stokes or anti-stokes detection. Analytical Biochemistry 227 309–318Google Scholar
Tian, Y, Su, F, Weber, W, Nandakumar, V, Shumway, BR, Jin, Y, Zhou, X, Holl, MR, Johnson, HR & Meldrum, DR 2010 A series of naphthalimide derivatives as intra and extracellular pH sensors. Biomaterials 31 7411–7422Google Scholar
Wolfbeis, OS 2008 Fiber-optic chemical sensors and biosensors. Analytical Chemistry 80 4269–4283Google Scholar