Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T13:09:55.214Z Has data issue: false hasContentIssue false
  • English
  • Français

Hepatic Sirt3 expression declines postpartum in dairy goats

Published online by Cambridge University Press:  22 May 2018

Lei Liu ,
Li Yao ,
Tao Peng ,
Lixin Wen ,
Wenjie Cai ,
Xinglin Jia  and
Jianhua He
Lei Liu
Affiliation:
College of Veterinary Medicine, Hunan Collaborative innovation center of safety production of livestock and poultry, Hunan Agricultural University, Changsha 410128, China College of Animal Science, Hunan Agricultural University, Changsha 410128, China
Li Yao
Affiliation:
College of Veterinary Medicine, Hunan Collaborative innovation center of safety production of livestock and poultry, Hunan Agricultural University, Changsha 410128, China
Tao Peng
Affiliation:
College of Veterinary Medicine, Hunan Collaborative innovation center of safety production of livestock and poultry, Hunan Agricultural University, Changsha 410128, China
Lixin Wen
Affiliation:
College of Veterinary Medicine, Hunan Collaborative innovation center of safety production of livestock and poultry, Hunan Agricultural University, Changsha 410128, China
Wenjie Cai
Affiliation:
Hunan Institute of Animal and Veterinary Science, Changsha 410128, China
Xinglin Jia*
Affiliation:
College of Veterinary Medicine, Hunan Collaborative innovation center of safety production of livestock and poultry, Hunan Agricultural University, Changsha 410128, China
Jianhua He*
Affiliation:
College of Animal Science, Hunan Agricultural University, Changsha 410128, China
*
*For correspondence; e-mail: [email protected][email protected]
*For correspondence; e-mail: [email protected][email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The experiments reported in this research communication aimed to plot the expression pattern of Sirt3, a master regulator of energy metabolism and antioxidation defence, in the liver of dairy goats during perinatal period. Ten healthy dairy goats in late pregnancy were chosen, and needle biopsy was applied to collect liver samples at 1-week intervals. Protein levels of hepatic Sirt3 were analysed by western-blotting. Serum enzyme activities of manganese superoxide dismutase (Mn-SOD) and non-esterified fatty acids (NEFA) levels were measured, and their correlation with Sirt3 mRNA levels was also estimated. Compared with >3-week before parturition (BP), Sirt3 proteins were significantly reduced at 1-week after parturition (AP) and 2-week AP (P < 0·05), but increased on the day of parturition (P < 0·01). Correlation analysis revealed a positive association between hepatic Sirt3 mRNA levels and serum enzyme activity of Mn-SOD (r = 0·46), but a negative association between that and serum NEFA levels (r = −0·41). These data indicate that the decreased hepatic expression of Sirt3 might be one of the reasons that dairy goats undergo oxidative stress after parturition.

Keywords

Dairyliveroxidative stressperinatal periodmetabolic diseases
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 163 - 166
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bause, AS & Haigis, MC 2013 SIRT3 regulation of mitochondrial oxidative stress. Experimental Gerontology 48 634CrossRefGoogle ScholarPubMed
Du, X, Shi, Z, Peng, Z, Zhao, C, Zhang, Y, Wang, Z, Li, X, Liu, G & Li, X 2017a Acetoacetate induces hepatocytes apoptosis by the ROS-mediated MAPKs pathway in ketotic cows. Journal of Cellular Physiology 232 3296–3308CrossRefGoogle ScholarPubMed
Du, X, Yang, Y, Xu, C, Peng, Z, Zhang, M, Lei, L, Gao, W, Dong, Y, Shi, Z, Sun, X, Wang, Z, Li, X, Li, X & Liu, G 2017b Upregulation of miR-181a impairs hepatic glucose and lipid homeostasis. Oncotarget 8 91362–91378CrossRefGoogle ScholarPubMed
Grummer, RR 1995 Impact of changes in organic nutrient metabolism on feeding the transition dairy cow. Journal of Animal Science 73 2820CrossRefGoogle ScholarPubMed
Hebert, AS, Dittenhafer-Reed, KE, Yu, W, Bailey, DJ, Selen, ES, Boersma, MD, Carson, JJ, Tonelli, M, Balloon, AJ, Higbee, AJ, Westphall, MS, Pagliarini, DJ, Prolla, TA, Assadi-Porter, F, Roy, S, Denu, JM & Coon, JJ 2013 Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome. Molecular Cell 49 186–199CrossRefGoogle ScholarPubMed
Qiu, X, Brown, K, Hirschey, MD, Verdin, E & Chen, D 2010 Calorie restriction reduces oxidative stress by SIRT3-mediated SOD2 activation. Cell Metabolism 12 662–667CrossRefGoogle ScholarPubMed
Radin, L, Šimpraga, M, Vince, S, Kostelić, A & Milinkovićtur, S 2015 Metabolic and oxidative status of Saanen goats of different parity during the peripartum period. Journal of Dairy Research 82 426–433CrossRefGoogle ScholarPubMed
Song, Y, Li, N, Gu, J, Fu, S, Peng, Z, Zhao, C, Zhang, Y, Li, X, Wang, Z & Li, X 2016 β-Hydroxybutyrate induces bovine hepatocyte apoptosis via an ROS-p38 signaling pathway. Journal of Dairy Science 99 9184CrossRefGoogle ScholarPubMed
Sun, LW, Zhang, HY, Wu, L, Shu, S, Xia, C, Xu, C & Zheng, JS 2014 (1)H-Nuclear magnetic resonance-based plasma metabolic profiling of dairy cows with clinical and subclinical ketosis. Journal of Dairy Science 97 1552CrossRefGoogle ScholarPubMed
Yao, L, Peng, T, Jiao, L, Liu, L, Xing, W, Jia, X, Yuan, A & Liu, L 2017 Effect of liver biopsy on blood routine and liver function in peripartum goats. Progress in Veterinary Medicine 38 128–131Google Scholar