Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-19T08:45:11.727Z Has data issue: false hasContentIssue false
  • English
  • Français

Haplotypic variation in the UCP1 gene is associated with milk traits in dairy cows

Published online by Cambridge University Press:  03 October 2016

Huitong Zhou ,
Long Cheng ,
Seung Ok Byun ,
Wumaierjiang Aizimu ,
Miriam C Hodge ,
Grant R. Edwards  and
Jon GH Hickford
Huitong Zhou*
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Long Cheng
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Seung Ok Byun
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Wumaierjiang Aizimu
Affiliation:
College of Animal Science, Tarim University, Alar 843300, Xinjiang Province, China
Miriam C Hodge
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Grant R. Edwards
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Jon GH Hickford
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Uncoupling protein-1 (UCP1) plays a role in the regulation of body temperature, metabolic rate and energy expenditure in animals. While variation in UCP1 and its phenotypic effect has been investigated in humans and sheep, little is known about this gene in cattle. In this study, four regions of bovine UCP1 were investigated in 612 Holstein-Friesian × Jersey (HF × J) dairy cows using polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analyses. In the four regions of the gene analysed, a total of 13 SNPs were detected. Three sequences (a, b and c) were found in Region-2 and three sequences (A, B and C) were found in Region-4, and these were assembled into three (a-B, b-B and c-A) common and three (b-C, c-B and c-C) rare haplotypes. Of the three common haplotypes, b-B and c-A were associated (P < 0·007 and P < 0·043, respectively) with increased milk yield and tended to be associated (P < 0·085 and P < 0·070, respectively) with decreased fat percentage. Cows with genotype b-B/a-B produced more milk (P < 0·004), but with a lower percentage of fat (P < 0·035) and protein (P < 0·038) than cows with genotype a-B/a-B. Cows of genotype a-B/c-A had milk of low fat percentage (P < 0·017), but tended to produce more milk (P < 0·059) than cows of genotype a-B/a-B. This suggests that UCP1 affects milk yield, milk fat percentage and milk protein percentage.

Keywords

Uncoupling protein-1 gene (UCP1)variation; haplotypemilk yieldmilk fatmilk protein
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 1 , February 2017 , pp. 68 - 75
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aguilar, I, Misztal, I & Tsuruta, S 2009 Genetic components of heat stress for dairy cattle with multiple lactations. Journal of Dairy Science 92 5702–5711 Google Scholar
Ahlborn, G & Dempfle, L 1992 Genetic parameters for milk production and body size in New Zealand Holstein-Friesian and Jersey. Livestock Production Science 31 205–219 CrossRefGoogle Scholar
Battagin, M, Sartori, C, Biffani, S, Penasa, M & Cassandro, M 2013 Genetic parameters for body condition score, locomotion, angularity, and production traits in Italian Holstein cattle. Journal of Dairy Science 96 5344–5351 CrossRefGoogle ScholarPubMed
Bluett, S, Fisher, A & Waugh, C 2000 Heat challenge of dairy cows in the Waikato: a comparison of spring and summer. In Proceeding of the New Zealand Society of Animal production, Volume 60, Hamilton, New Zealand, Dairying Research Corporation, pp. 226–229 Google Scholar
Bohmanova, J, Misztal, I, Tsuruta, S, Norman, HD & Lawlor, TJ 2008 Short communication: genotype by environment interaction due to heat stress. Journal of Dairy Science 91 840–846 Google Scholar
Boonkum, W, Misztal, I, Duangjinda, M, Pattarajinda, V, Tumwasorn, S & Sanpote, J 2011 Genetic effects of heat stress on milk yield of Thai Holstein crossbreds. Journal of Dairy Science 94 487–492 Google Scholar
Brondani, LA, Assmann, TS, Duarte, GCK, Gross, JL, Canani, LH & Crispim, D 2012 The role of the uncoupling protein 1 (UCP1) on the development of obesity and type 2 diabetes mellitus. Arquivos Brasileiros de Endocrinologia & Metabologia 56 215–225 Google Scholar
Brügemann, K, Gernand, E, von Borstel, UU & König, S 2011 Genetic analyses of protein yield in dairy cows applying random regression models with time-dependent and temperature x humidity-dependent covariates. Journal of Dairy Science 94 4129–4139 Google Scholar
Bryant, JR, Lόpez-Villalobos, N, Pryce, JE, Holmes, CW 2007 Quantifying the effect of thermal environment on production traits on three breeds of dairy cattle in New Zealand. New Zealand Journal of Agricultural Research 50 327–338 Google Scholar
Byun, SO, Fang, Q, Zhou, H & Hickford, JGH 2009 An effective method for silver-staining DNA in large numbers of polyacrylamide gels. Analytical Biochemistry 385 174–175 Google Scholar
Cannon, B & Nedergaard, J 2004 Brown adipose tissue: function and physiological significance. Physiological Reviews 84 277–359 Google Scholar
Casteilla, L, Bouillaud, F, Forest, C & Ricquier, D 1989 Nucleotide sequence of a cDNA encoding bovine brown fat uncoupling protein. Homology with ADP binding site of ADP/ATP carrier. Nucleic Acids Research 17 2131 Google Scholar
Dikmen, S & Hansen, PJ 2009 Is the temperature-humidity index the best indicator of heat stress in lactating dairy cows in a subtropical environment? Journal of Dairy Science 92 109–116 CrossRefGoogle Scholar
Enerback, S, Jacobsson, A, Simpson, E, Guerra, C, Yamashita, H, Harper, M & Kozak, L 1997 Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. Nature 387 90–93 Google Scholar
Feldmann, HM, Golozoubova, V, Cannon, B & Nedergaard, J 2009 UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality. Cell Metabolism 9 203–209 CrossRefGoogle ScholarPubMed
Hammami, H, Bormann, J, M'hamdi, N, Montaldo, HH & Gengler, N 2013 Evaluation of heat stress effects on production traits and somatic cell score of Holsteins in a temperate environment. Journal of Dairy Science 96 1844–1855 Google Scholar
Hammami, H, Vandenplas, J, Vanrobays, M-L, Rekik, B, Bastin, C & Gengler, N 2015 Genetic analysis of heat stress effects on yield traits, udder health, and fatty acids of Walloon Holstein cows. Journal of Dairy Science 98 4956–4968 CrossRefGoogle ScholarPubMed
Jia, JJ, Tian, YB, Cao, ZH, Tao, L, Zhang, X, Gao, SZ, Ge, CR, Lin, QY & Jois, M 2010 The polymorphisms of UCP1 genes associated with fat metabolism, obesity and diabetes. Molecular Biology Reports 37 1513–1522 Google Scholar
Kadzere, C, Murphy, M, Silanikove, N & Maltz, E 2002 Heat stress in lactating dairy cows: a review. Livestock Production Science 77 59–91 Google Scholar
Kendall, PE, Verkerk, GA, Webster, JR & Tucker, CB 2007 Sprinklers and shade cool cows and reduce insect-avoidance behavior in pasture-based dairy systems. Journal of Dairy Science 90 3671–3680 Google Scholar
Kozak, L, Koza, R & Anunciado-Koza, R 2010 Brown fat thermogenesis and body weight regulation in mice: relevance to humans. International Journal of Obesity 34 S23–S27 Google Scholar
Król, E & Speakman, J 2003 Limits to sustained energy intake VI. Energetics of lactation in laboratory mice at thermoneutrality. Journal of Experimental Biology 206 4255–4266 Google Scholar
Król, E, Martin, SA, Huhtaniemi, IT, Douglas, A & Speakman, JR 2011 Negative correlation between milk production and brown adipose tissue gene expression in lactating mice. Journal of Experimental Biology 214 4160–4170 Google Scholar
Loker, S, Bastin, C, Miglior, F, Sewalem, A, Schaeffer, L, Jamrozik, J, Ali, A & Osborne, V 2012 Genetic and environmental relationships between body condition score and milk production traits in Canadian Holsteins. Journal of Dairy Science 95 410–419 Google Scholar
Macdonald, K, Verkerk, G, Thorrold, B, Pryce, J, Penno, J, McNaughton, L, Burton, L, Lancaster, J, Williamson, J & Holmes, C 2008 A comparison of three strains of Holstein-Friesian grazed on pasture and managed under different feed allowances. Journal of Dairy Science 91 1693–1707 CrossRefGoogle ScholarPubMed
Nizielski, SE, Billington, CJ & Levine, AS 1993 BAT thermogenic activity and capacity are reduced during lactation in ground squirrels. American Journal of Physiology 264 R16–R21 Google Scholar
Ravagnolo, O & Misztal, I 2000 Genetic component of heat stress in dairy cattle, parameter estimation. Journal of Dairy Science 83 2126–2130 CrossRefGoogle ScholarPubMed
Renna, M, Lussiana, C, Malfatto, V, Mimosi, A & Mattaglini, LM 2010 Effect of exposure to heat stress conditions on milk yield and quality of dairy cows grazing on Alpine pasture. In Proceedings of 9th European IFSA Symposium, pp. 1338–1348 (Ed. Darnhoefer, I & Grotzer, M). Vienna, Austria Google Scholar
Roenfeldt, S 1998 You can't afford to ignore heat stress. Dairy Manage 35 6–12 Google Scholar
Sánchez, JP, Misztal, I, Aguilar, I, Zumbach, B & Rekaya, R 2009 Genetic determination of the onset of heat stress on daily milk production in the US Holstein cattle. Journal of Dairy Science 92 4035–4045 Google Scholar
Sonstegard, T & Kappes, S 1999 Mapping of the UCP1 locus to bovine chromosome 17. Animal Genetics 30 462–478 Google Scholar
Speakman, JR & Król, E 2005 Limits to sustained energy intake IX: a review of hypotheses. Journal of Comparative Physiology B 175 375–394 Google Scholar
Thatcher, WW & Collier, RJ 1986 Effects of climate on bovine reproduction. In Current Therapy in Theriogenology: Diseases, Treatment, and Prevention of Reproductive Diseases in Small and Large Animals, 2nd edition. pp. 301–309 (Ed. Morrow, DA). Philadelphia, PA: W. B. Saunders Google Scholar
Trayhurn, P, Douglas, JB & McGuckin, MM 1982 Brown adipose tissue thermogenesis is ‘suppressed’ during lactation in mice. Nature 298 59–60 Google Scholar
Yang, G, Forrest, R, Zhou, H, Hodge, S & Hickford, J 2014 Genetic variation in the ovine uncoupling protein 1 gene: association with carcass traits in New Zealand (NZ) Romney sheep, but no association with growth traits in either NZ Romney or NZ Suffolk sheep. Journal of Animal Breeding and Genetics 131 437–444 Google Scholar
Yuan, YN, Liu, WZ, Liu, JH, Qiao, LY & Wu, JL 2012 Cloning and ontogenetic expression of the uncoupling protein 1 gene UCP1 in sheep. Journal of Applied Genetics 53 203–212 Google Scholar
Zhou, H, Hickford, JGH & Fang, Q 2006 A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification. Analytical Biochemistry 354 159–161 Google Scholar
Zhou, H, Li, S, Liu, X, Wang, J, Luo, Y & Hickford, JG 2014 Haplotyping using a combination of polymerase chain reaction-single-strand conformational polymorphism analysis and haplotype-specific PCR amplification. Analytical Biochemistry 466 59–64 Google Scholar