Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-20T06:41:59.395Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic variation at the goat hormone-sensitive lipase (LIPE) gene and its association with milk yield and composition

Published online by Cambridge University Press:  12 April 2010

Ali Zidi ,
Víctor M Fernández-Cabanás ,
Juan Carrizosa ,
Jordi Jordana ,
Baltasar Urrutia ,
Oliva Polvillo ,
Pedro González-Redondo ,
David Gallardo ,
Marcel Amills  and
Juan M Serradilla
Ali Zidi
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Víctor M Fernández-Cabanás
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Spain
Juan Carrizosa
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA). Estación Sericícola. La Alberca, Murcia, Spain
Jordi Jordana
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Baltasar Urrutia
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA). Estación Sericícola. La Alberca, Murcia, Spain
Oliva Polvillo
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Spain
Pedro González-Redondo
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Spain
David Gallardo
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Marcel Amills*
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193Bellaterra, Spain
Juan M Serradilla*
Affiliation:
Departamento de Producción Animal, Campus de Rabanales, Universidad de Córdoba, 14071Córdoba, Spain
*
*For correspondence; e-mail: [email protected]
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Hormone-sensitive lipase (LIPE) plays a fundamental role in the regulation of energy balance by releasing free fatty acids from adipose triacylglycerol stores. These fatty acids can be subsequently transferred to other body compartments to be oxidized or employed in other biochemical reactions. This enzymic function is particularly important in lactating animals because the synthesis of milk components involves the mobilization of lipid depots to satisfy the large energy demands of the mammary gland. In the current study, we partially sequenced the goat LIPE gene in several individuals. In doing so, we identified two synonymous polymorphisms at exons 2 (c.327C>A>T, triallelic polymorphism) and 3 (c.558C>T). Moreover, we found a mis-sense polymorphism at exon 6 (c.1162G>T) that involves an alanine to serine substitution at position 388. Analysis with Polyphen and Panther softwares revealed that this amino acid replacement is expected to be neutral. Performance of an association analysis with a variety of milk traits revealed that goat LIPE genotype has highly suggestive effects on milk yield (P=0·0032) as well as on C18:3 n-6g (P=0·0051), trans-10 cis-12 CLA (P=0·007) and C12:0 (P=0·0084) milk contents. These associations are concordant with the preference of LIPE to selectively mobilize medium-chain and unsaturated fatty acids.

Keywords

Hormone-sensitive lipasegenemilk traitsgoat
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 2 , May 2010 , pp. 190 - 198
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Badaoui, B, Serradilla, JM, Tomàs, A, Urrutia, B, Ares, JL, Carrizosa, J, Sànchez, A, Jordana, J & Amills, M 2007 Goat acetyl-coenzyme A carboxylase α: Molecular characterization, polymorphism, and association with milk traits. Journal of Dairy Science 90 1039–1043Google Scholar
Blom, N, Gammeltoft, S & Brunak, S 1999 Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites. Journal of Molecular Biology 294 1351–1362CrossRefGoogle ScholarPubMed
Brenner, S 1988 The molecular evolution of genes and proteins: a tale of two serines. Nature 334 528–530CrossRefGoogle ScholarPubMed
Caravaca, F, Carrizosa, J, Urrutia, B, Baena, F, Jordana, J, Amills, M, Badaoui, B, Sànchez, A, Angiolillo, A & Serradilla, JM 2009 Effect of αS1-casein (CSN1S1) and κ-casein (CSN3) genotypes on milk composition in Murciano-Granadina goats. Journal of Dairy Science 92 2960–2964Google Scholar
Cook, KG, Yeaman, SJ, Strålfors, P, Fredrikson, G & Belfrage, P 1982 Direct evidence that cholesterol ester hydrolase from adrenal cortex is the same enzyme as hormone-sensitive lipase from adipose tissue. European Journal of Biochemistry 125 245–249Google Scholar
Fan, YY & Chapkin, RS 1998 Importance of dietary γ-linolenic acid in human health and nutrition. Journal of Nutrition 128 1411–1414Google Scholar
Garenc, C, Vohl, MC, Bouchard, C & Pérusse, L 2009 LIPE C-60G influences the effects of physical activity on body fat and plasma lipid concentrations: the Quebec Family Study. Human Genomics 3 157–168CrossRefGoogle ScholarPubMed
Garton, JA, Campbell, GD, Carling, D, Hardie, DG, Colbran, RJ & Yeaman, SJ 1989 Phosphorylation of bovine hormone-sensitive lipase by the AMP-activated protein kinase. A possible antilipolytic mechanism. European Journal of Biochemistry 179 249–254Google Scholar
Garton, JA & Yeaman, SJ 1990 Identification and role of the basal phosphorylation site on hormone-sensitive lipase. European Journal of Biochemistry 191 245–250Google Scholar
Gavino, VC & Gavino, GR 1992 Adipose hormone-sensitive lipase preferentially releases polyunsaturated fatty acids from triglycerides. Lipids 27 950–954Google Scholar
Jensen, RG 2002 The composition of bovine milk lipids: January 1995 to December 2000. Journal of Dairy Science 85 295–350Google Scholar
Kraemer, FB & Shen, WJ 2002 Hormone-sensitive lipase: control of intracellular tri-(di-) acylglycerol and cholesteryl ester hydrolysis. Journal of Lipid Research 43 1585–1594Google Scholar
Lampidonis, AD, Argyrokastritis, A, Stravopodis, DJ, Voutsinas, GE, Ntouroupi, TG, Margaritis, LH, Bizelis, I & Rogdakis, E 2008 Cloning and functional characterization of the ovine hormone sensitive lipase (LIPE) full-length cDNAs: an integrated approach. Gene 416 30–43Google Scholar
Littell, RC, Henry, PR & Ammerman, CB 1998. Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science 76 1216–1231Google Scholar
Mel'uchová, B, Blaško, J, Kubinec, R, Górová, R, Dubravská, J, Margetín, M & Soják, L 2008 Seasonal variations in fatty acid composition of pasture forage plants and CLA content in ewe milk fat. Small Ruminant Research 78 56–65Google Scholar
Moate, PJ, Chalupa, W, RC, Boston & Lean, IJ 2007 Milk fatty acids. I. Variation in the concentration of individual fatty acids in bovine milk. Journal of Dairy Science 90 4730–4739CrossRefGoogle ScholarPubMed
Qi, L, Shen, H, Larson, I, Barnard, JR, Schaefer, EJ & Ordovas, JM 2004 Genetic variation at the hormone sensitive lipase: gender-specific association with plasma lipid and glucose concentrations. Clinical Genetics 65 93–100Google Scholar
Raclot, T & Groscolas, R 1993 Differential mobilization of white adipose tissue fatty acids according to chain length, unsaturation, and positional isomerism. Journal of Lipid Research 34 1515–1526Google Scholar
Ramensky, V, Bork, P & Sunyaev, S 2002 Human non-synonymous SNPs: server and survey. Nucleic Acids Research 30 3894–3900Google Scholar
Stephens, M, Smith, N & Donnelly, P 2001 A new statistical method for haplotype reconstruction from population data. American Journal of Human Genetics 68 978–989Google Scholar
Sukhija, PH & Palmquist, DL 1998 Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36 1202–1206Google Scholar
Thomas, PD & Kejariwal, A 2004 Coding single-nucleotide polymorphisms associated with complex vs. Mendelian disease: Evolutionary evidence for differences in molecular effects. Proceedings of the National Academy of Sciences of the United States of America 101 15398–15403Google Scholar
Vernon, RG, Clegg, RA & Flint, DJ 1981 Metabolism of sheep adipose tissue during pregnancy and lactation. Adaptation and regulation. Biochemical Journal 200 307–314Google Scholar
Wang, S, Soni, KG, Semache, M, Casavant, S, Fortier, M, Pan, L & Mitchell, GA 2008 Lipolysis and the integrated physiology of lipid energy metabolism. Molecular Genetics and Metabolism 95 117–126Google Scholar
Wu, Z & Huber, JT 1994 Relationship between dietary fat supplementation and milk protein concentration in lactating cows: A review. Livestock Production Science 39 141–155CrossRefGoogle Scholar
Yeaman, JS 2004 Hormone-sensitive lipase: new roles for an old enzyme. Biochemical Journal 379 11–22Google Scholar
Yonezawa, T, Haga, S, Kobayashi, Y, Katoh, K & Obara, Y 2008 Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells. Biochemical and Biophysical Research Communications 376 36–39Google Scholar