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Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle

Published online by Cambridge University Press:  29 July 2009

Maria Selvaggi ,
Cataldo Dario ,
Giovanni Normanno ,
Gaetano V Celano  and
Marco Dario
Maria Selvaggi
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Cataldo Dario*
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Giovanni Normanno
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Gaetano V Celano
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Marco Dario
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

STATs are a group of transcription factors that mediate actions of a variety of peptide hormones and cytokines within target cells (for example, prolactin and growth hormone). Therefore, STAT5A gene is a candidate marker for quantitative traits in farm animals with respect to milk production traits. In this study the STAT5A/AvaI polymorphism was investigated with PCR-RFLP in a sample of 233 Italian Brown cattle. This polymorphism is localized in the coding region of the bovine STAT5A gene. It is a substitution C→T at position 6853 within exon 7. All three possible genotypes for the C/T polymorphism were identified. The overall frequencies of alleles C and T were 0·83 and 0·17 respectively; the Hardy-Weinberg equilibrium was verified. In order to study the relationship between STAT5A/AvaI polymorphism and milk performance traits, the data for a 305-d milk production that included milk yield, protein and fat yield, fat and protein percentage were used. Significant differences between the two genotypes were found in yields of milk, fat and protein and protein percentage (P<0·01). CC cows produced more milk than CT (5418·68 v. 5149·54 kg). Protein content was higher in milk from CC compared with CT genotypes (3·40 v. 3·21%). No significant difference was found in fat content. Owing to the low number of TT cows in the studied population, this genotype was not included in the statistical analysis; in fact the number of TT cows was not enough to provide an accurate statistical analysis. Although more studies are needed to better clarify the role of this SNP on production traits, STAT5A/AvaI polymorphism appears to be a promising indirect marker to improve milk production traits in cattle.

Keywords

STAT5Agene polymorphismItalian Brown cattlemilk production traits
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 4 , November 2009 , pp. 441 - 445
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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