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Extensive countrywide field investigation of somatic cell counts and total bacterial counts in bulk tank raw milk in goat herds in Greece

Published online by Cambridge University Press:  16 August 2021

Daphne T. Lianou ,
Charalambia K. Michael ,
Natalia G.C. Vasileiou ,
Efthimia Petinaki ,
Peter J. Cripps ,
Katerina Tsilipounidaki ,
Angeliki I. Katsafadou ,
Antonis P. Politis ,
Nikos G. Kordalis  and
Katerina S. Ioannidi
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Daphne T. Lianou
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Charalambia K. Michael
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Natalia G.C. Vasileiou
Affiliation:
Faculty of Animal Science, University of Thessaly, 41110Larissa, Greece
Efthimia Petinaki
Affiliation:
University Hospital of Larissa, 41110Larissa, Greece
Peter J. Cripps
Affiliation:
Institute of Veterinary Science, University of Liverpool, NestonCH64 7TE, UK
Katerina Tsilipounidaki
Affiliation:
University Hospital of Larissa, 41110Larissa, Greece
Angeliki I. Katsafadou
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Antonis P. Politis
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Nikos G. Kordalis
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Katerina S. Ioannidi
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Dimitris A. Gougoulis
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Constantina Trikalinou
Affiliation:
University Hospital of Larissa, 41110Larissa, Greece
Denise C. Orfanou
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Ilektra A. Fragkou
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Dimitra V. Liagka
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Athina Tzora
Affiliation:
Faculty of Agriculture, University of Ioannina, 47100Arta, Greece
Marzia Albenzio
Affiliation:
Department of Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, 71122Foggia, Italy
Vasia S. Mavrogianni
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
Mariangela C. Caroprese
Affiliation:
Department of Sciences of Agriculture, Food and Environment (SAFE), University of Foggia, 71122Foggia, Italy
George C. Fthenakis*
Affiliation:
Veterinary Faculty, University of Thessaly, 43100Karditsa, Greece
*
Author for correspondence: George C. Fthenakis, Email: [email protected]
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Abstract

Dairy goat farming is an important sector of the agricultural industry in Greece, with an annual total milk production exceeding 450 000 l and accounting for over 25% of all goat milk produced in the European Union; this milk is used mainly for cheese production. Despite the importance of goat milk for the agricultural sector in Greece, no systematic countrywide investigations in the bulk-tank milk of goats in Greece have been reported. Objectives were to investigate somatic cell counts (SCC) and total bacterial counts (TBC) in raw bulk-tank milk of goat herds in Greece, study factors influencing SCC and TBC therein and evaluate their possible associations with milk content. Throughout Greece, 119 dairy goat herds were visited for milk sampling for somatic cell counting, microbiological examination and composition measurement. Geometric mean SCC and TBC were 0.838 × 106 cells ml−1 and 581 × 103 cfu ml−1, respectively. Multivariable analyses revealed annual frequency of check-ups of milking system and total milk quantity per goat (among 53 variables) to be significant for increased SCC; no factor emerged (among 58 variables) to be significant for increased TBC. Negative correlation of SCC with total protein was found; mean total protein content in the bulk-tank milk in herds with SCC >0.75 × 106 cells ml−1 was 5.1% lower and in herds with SCC >1.5 × 106 cells ml−1, it was 7.8% lower.

Keywords

Bulk-tankgoatmastitisprotein contentraw milksheepslimesubclinical mastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 307 - 313
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Albenzio, M, Taibi, L, Caroprese, M, De Rosa, G, Muscio, A and Sevi, A (2003) Immune response, udder health and productive traits of machine milked and suckling ewes. Small Ruminant Research 48, 189–200.CrossRefGoogle Scholar
Albenzio, M, Santillo, A, Caroprese, M, Schena, L, Russo, D and Sevi, A (2011) Composition, indigenous proteolytic enzymes and coagulating behaviour of ewe milk as affected by somatic cell count. Journal of Dairy Research 78, 442–447.10.1017/S0022029911000525CrossRefGoogle ScholarPubMed
Anyfantakis, EM (2004) Cheese Production: Chemistry, Physicochemistry, Microbiology, 2nd Edn., Athens, Greece: Stamoulis Publications.Google Scholar
Barrow, GI and Feltham, RKA (1993) Manual for the Identification of Medical Bacteria, 3rd Edn. Cambridge, UK: Cambridge University Press.CrossRefGoogle Scholar
Besier, J, Lind, O and Bruckmaier, RM (2016) Dynamics of teat-end vacuum during machine milking: types, causes and impacts on teat condition and udder health – a literature review. Journal of Applied Animal Research 44, 263–272.CrossRefGoogle Scholar
Caroprese, Μ, Giannenas, Ι and Fthenakis, GC (2015) Interactions between nutritional approaches and defences against microbial diseases in small ruminants. Veterinary Microbiology 181, 8–14.CrossRefGoogle ScholarPubMed
Contreras, A, Luengo, C, Sanchez, A and Corrales, JC (2003) The role of intramammary pathogens in dairy goats. Livestock Production Science 79, 273–283.CrossRefGoogle Scholar
European Union (2004) Regulation (EC) No. 853/2004 of the European Parliament and of the council of 29 April 2004 laying down specific hygiene rules for on the hygiene of foodstuffs. Official Journal of the European Union 47, L 139/55–205.Google Scholar
Euzeby, JP (1997) List of bacterial names with standing in nomenclature: a folder available on the internet. International Journal of Systematic Bacteriology 47, 590–592.Google ScholarPubMed
Federation of Veterinarians of Europe (2009) Veterinary Act – European Veterinary Code of Conduct. Brussels, Belgium: Federation of Veterinarians in Europe.Google Scholar
Gonzalo, C, Ariznabarreta, A, Carriedo, JA and San Primitivo, F (2002) Mammary pathogens and their relationship to somatic cell count and milk yield losses in dairy ewes. Journal of Dairy Science 85, 1460–1467.10.3168/jds.S0022-0302(02)74214-8CrossRefGoogle ScholarPubMed
Gosselin, VB, Dufour, S, Adkins, PRF and Middleton, JR (2019) Persistence of coagulase negative staphylococcal intramammary infections in dairy goats. Journal of Dairy Research 86, 211–216.CrossRefGoogle Scholar
Haenlein, GFW (2002) Relationship of somatic cell counts in goat milk to mastitis and productivity. Small Ruminant Research 45, 163–178.CrossRefGoogle Scholar
Hellenic Agricultural Organisation—Demeter (2020) Deliveries of Ovine and Caprine Milk by Region and Regional Authority and Average Milk Price—Calendar Year 2019. Cumulative Data Updated. Available online: https://www.elgo.gr/images/ELOGAK_files/Statistics/2020/AIGO_Παραδόσεις_Πρόβειου_και_Γίδινου_Γάλακτος_2019.pdf.Google Scholar
Hellenic Veterinary Association (2011) Code of Correct Veterinary Practice. Athens, Greece: Hellenic Veterinary Association.Google Scholar
Jimenez-Granado, R, Sanchez-Rodriguez, M, Arce, C and Rodriguez-Estevez, V (2014) Factors affecting somatic cell count in dairy goats: a review. Spanish Journal of Agricultural Research 12, 133–150.CrossRefGoogle Scholar
Kenyon, PR, Maloney, SK and Blache, D (2014) Review of sheep body condition score in relation to production characteristics. New Zealand Journal of Agricultural Research 57, 38–64.10.1080/00288233.2013.857698CrossRefGoogle Scholar
Koop, G, Nielen, M and van Werven, T (2009) Bulk milk somatic cell counts are related to bulk milk total bacterial counts and several herd-level risk factors in dairy goats. Journal of Dairy Science 92, 4355–4364.CrossRefGoogle ScholarPubMed
Koop, G, van Werven, T, Schuiling, HJ and Nielen, M (2010) The effect of subclinical mastitis on milk yield in dairy goats. Journal of Dairy Science 93, 5809–5817.CrossRefGoogle ScholarPubMed
Koop, G, De Vliegher, S, De Visscher, A, Supré, K, Haesebrouck, F, Nielen, M and van Werven, T (2012) Differences between coagulase-negative Staphylococcus species in persistence and in effect on somatic cell count and milk yield in dairy goats. Journal of Dairy Science 95, 5075–5084.CrossRefGoogle ScholarPubMed
Laird, DT, Gambrel-Lenarz, SA, Scher, FM, Graham, TE and Reddy, R (2004) Microbiological count methods. In Wehr, HM, Frank, JF (eds), Standard Methods for the Examination of Dairy Products, 17th Edn. Washington, USA: APHA Press, pp. 153–186.Google Scholar
Leitner, G, Lavon, Y, Matzrafi, Z, Benun, O, Bezman, D and Merin, U (2016) Somatic cell counts, chemical composition and coagulation properties of goat and sheep bulk tank milk. International Dairy Journal 58, 9–13.CrossRefGoogle Scholar
Lianou, DT, Chatziprodromidou, IP, Vasileiou, NGC, Michael, CK, Mavrogianni, VS, Politis, AP, Kordalis, NG, Billinis, C, Giannakopoulos, A, Papadopoulos, E, Giannenas, I, Ioannidi, KS, Katsafadou, AI, Gougoulis, DA, Lacasta, D, Caroprese, M and Fthenakis, GC (2020) A detailed questionnaire for the evaluation of health management in dairy sheep and goats. Animals 10, 1489.CrossRefGoogle ScholarPubMed
Lira, MC, Givisiez, PEN, de Sousa, FGC, Magnani, M, de Souza, EL, Spricigo, DA, Gebreyes, WA and Bruno de Oliveira, CJB (2016) Biofilm-forming and antimicrobial resistance traits of staphylococci isolated from goat dairy plants. The Journal of Infection in Developing Countries 10, 932–936.CrossRefGoogle ScholarPubMed
Martinez, B (2000) Somatic cell counts in the milk of goats, factors of variation and effect on the production and composition of milk. PhD thesis, Polytechnic University of Valencia, Valencia, Spain.Google Scholar
Martins, L, Barcelos, MM, Cue, RI, Anderson, KL, dos Santos, MV and Goncalves, JL (2020) Chronic subclinical mastitis reduces milk and components yield at the cow level. Journal of Dairy Research 87, 298–305.CrossRefGoogle Scholar
Moroni, P, Pisoni, G, Ruffo, G and Boettcher, PJ (2005) Risk factors for intramammary infections and relationship with somatic-cell counts in Italian dairy goats. Preventive Veterinary Medicine 69, 163–173.CrossRefGoogle ScholarPubMed
Papadopoulos, A (2009) Changes of characteristics of mechanically produced ovine milk from the udder to the bulk-tank. MSc thesis, Aristotle University of Thessaloniki. Thessaloniki, Greece.Google Scholar
Popescu, A (2013) Study regarding the trends in the world and European goat milk production. Lucrări Ştiinţifice-Seria Zootehnie 59, 127–132.Google Scholar
Romero, G, Peris, C, Fthenakis, GC and Diaz, JR (2020) Effects of machine milking on udder health in dairy ewes. Small Ruminant Research 188, 106096.CrossRefGoogle Scholar
Sandrucci, A, Bava, L, Tamburini, A, Gislon, G and Zucali, M (2019) Management practices and milk quality in dairy goat farms in Northern Italy. Italian Journal of Animal Science 18, 1–12.CrossRefGoogle Scholar
Saratsis, P, Alexopoulos, C, Tzora, A and Fthenakis, GC (1999) The effect of experimentally induced subclinical mastitis on the milk yield of dairy ewes. Small Ruminant Research 32, 205–209.CrossRefGoogle Scholar
Sevi, A, Albenzio, M, Taibi, L, Dantone, D, Massa, S and Annicchiarico, G (1999) Changes of somatic cell count through lactation and their effects on nutritional, renneting and bacteriological characteristics of ewe's milk. Advances in Food Sciences 21, 122–127.Google Scholar
US Department of Health and Human Services (2017) Grade “A” Pasteurized Milk Ordinance, 2017 revision. U.S. Department of Health and Human Services, Public Health Service, Food and Drug Administration, United States of America.Google Scholar
Vasileiou, NGC, Chatzopoulos, DC, Gougoulis, DA, Sarrou, S, Katsafadou, AI, Spyrou, V, Mavrogianni, VS, Petinaki, E and Fthenakis, GC (2018) Slime-producing staphylococci as causal agents of subclinical mastitis in sheep. Veterinary Microbiology 224, 93–99.CrossRefGoogle ScholarPubMed
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