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Enzymatic-fluorometric analyses for glutamine, glutamate and free amino groups in protein-free plasma and milk

Published online by Cambridge University Press:  02 March 2017

Torben Larsen  and
Carlos Fernández
Torben Larsen*
Affiliation:
Department of Animal Science, Aarhus University, Blichers Allé 20, PO Box 50, 8830 Tjele, Denmark
Carlos Fernández
Affiliation:
ACUMA Research Center, Polytechnic University of Valencia, 46022 Valencia, Spain
*
*For correspondence; e-mail: [email protected]
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Abstract

This Technical Research Communication describes new analytical methods for free, unbound glutamic acid and glutamine in protein-free blood plasma and milk and introduces the use of quantitation of free amino groups in the same matrices for descriptive and analytical purposes. The present enzymatic-fluorometric methods are easily performed within one working day, allowing for ‘high throughput’ assays of animal trials. These assays could support and enable further studies in lactation physiology with the objective of improved metabolic health.

Keywords

Milk metabolitesbiomarkersGlnGluOPA
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 1 , February 2017 , pp. 32 - 35
Copyright
Copyright © Proprietors of Journal of Dairy Research 2017 

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References

Dunn, MS & Ross, FJ 1938 Quantitative investigations of amino acids and peptides: IV. The solubilities of the amino acids in water-ethyl alchohol mixtures. Journal of Biological Chemistry 125 309–332 Google Scholar
Heitmann, RN & Bergman, EN 1980 Integration of amino acid metabolism in sheep: effects of fasting and acidosis. American Journal of Physiology 239 E248–E254 Google Scholar
Imhasly, S, Naegeli, H, Bauman, S, von Bergen, M, Luch, A, Jungnickel, H, Potratz, S & Gerspach, C 2014 Metabolomic biomarkers correlating with hepatic lipidosis in dairy cows. BMC Veterinary Research 10 122 CrossRefGoogle ScholarPubMed
Lacey, JM & Wilmore, D 1990 Is glutamine a conditionally essential amino acid? Nutrition Reviews 48 297–309 Google Scholar
Lund, P 1990 2.6.1. L-glutamine and L-glutamate, UV-method with glutaminase and glutamate dehydrogenase. pp. 357–363. In: Methods of Enzymatic Analysis, 3rdedn, Vol. VIII (Ed. Bergmeyer, HU). Weinheim, Germany: VCH Verlagsgesellschaft mbH D-6940 Google Scholar
Meijer, GAL, van der Meulen, J, Bakker, JGM, van der Koelen, CJ & van Vuuren, AM 1995 Free amino acids in plasma and muscle of high yielding dairy cows in early lactation. Journal of Dairy Science 78 1131–1141 Google Scholar
Nozaki, Y & Tanford, C 1971 The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. Journal of Biological Chemistry 246 2211–2217 Google Scholar
Plaizier, JC, Walton, J-P & McBride, BW 2001 Effect of post-ruminal infusion of glutmine on plasma amino acids, milk yield and composition in lactating dairy cows. Canadian Journal of Animal Science 81 229–235 Google Scholar
Roth, M 1971 Fluorescence reaction for amino acids. Analytical Chemistry 43 880–882 CrossRefGoogle ScholarPubMed
Torres dʼPaula, J, Martinez, RLV, de Almeida Craveiro Alves, TL, Mélo, SKM, Kutschenko, M, Nogueira, ET, da Costa Cordeira Manso, HEC & Filho, HCM 2014 Blood and milk glutamine + glutamate and milk composition in lactating Holstein cows in semi-arid of Brazil. Open Journal of Veterinary Medicine 4 322–328 CrossRefGoogle Scholar