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Efficacy of standard vs. extended intramammary cefquinome treatment of clinical mastitis in cows with persistent high somatic cell counts

Published online by Cambridge University Press:  17 September 2014

Jantijn M Swinkels ,
Volker Krömker  and
Theo JGM Lam
Jantijn M Swinkels*
Affiliation:
MSD Animal Health, Global Ruminant Business Unit, 5830 AA, Boxmeer, The Netherlands
Volker Krömker
Affiliation:
Department of Bioprocess Engineering, Microbiology, Hannover University of Applied Sciences, D-30453, Hannover, Germany
Theo JGM Lam
Affiliation:
GD Animal Health, Research and Development Department, 7400 AA, Deventer and Utrecht University, Faculty of Veterinary Medicine, Department of Farm Animal Health, 3584 CL, Utrecht, The Netherlands
*
*For correspondence; e-mail: [email protected]
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Abstract

Extended duration of clinical mastitis (CM) treatment has been advocated, although results showing its higher efficacy compared with standard treatment are difficult to compare and seem conflicting. In a non-blinded, positively controlled clinical trial with systematic allocation, the efficacy of a standard, 1·5-d cefquinome treatment (ST), and an extended, 5-d intramammary cefquinome treatment (ET) were evaluated. The latter is frequently performed in cows with persistent high somatic cell count (SCC), expecting a better cure. Therefore, cows with CM immediately preceded by at least two consecutive monthly elevated SCC >200 000 cells/ml, were studied. The primary efficacy criteria were bacteriological cure (BC) and clinical cure (CC), while SCC cure was considered a secondary criterion of cure. Least square means of overall BC were not different after ET (79%, n=206) compared with ST (72%, n=203). ET, as compared with ST, improved BC of CM when caused by streptococci, specifically Streptococcus uberis. At day 1·5, only 13% of quarters showed CC, increasing significantly towards 60% at day 5, and 99% at day 14 and at day 21. No significant difference in CC was present between treatment groups. Overall SCC cure was low (22%) and not significantly different between treatment groups, but significantly higher for cases due to enterobacteriacae compared with staphylococci. In conclusion, ET with cefquinome of CM in cows with a persistent high SCC seems to be only indicated when caused by streptococci, mainly Str. uberis but shows no advantage when no information on bacteriological causes of mastitis is available. In our data, absence of CC directly after ST was not related to eventual BC.

Keywords

Dairy cowlactationextended therapyclinical mastitisantimicrobial
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 4 , November 2014 , pp. 424 - 433
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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References

Barkema, HW, Deluyker, HW, Schukken, YH & Lam, TJGM 1999 Quarter-milk somatic cell count at calving and at the first six milkings after calving. Preventive Veterinary Medicine 38 1–9 CrossRefGoogle ScholarPubMed
Barlow, JW, White, LJ, Zadoks, RN & Schukken, YH 2009 A mathematical model demonstrating indirect and overall effects of lactation therapy targeting subclinical mastitis in dairy herds. Preventive Veterinary Medicine 90 31–42 Google Scholar
Bradley, AJ & Green, MJ 2001 Adaptation of Escherichia coli to the bovine mammary gland. Journal of Clinical Microbiology 39 1845–1849 Google Scholar
Committee for Medicinal products for Veterinary Use (CVMP) 2013 Guideline on the conduct of efficacy studies for intramammary products for use in cattle. Report EMEA/CVMP/EWP/141272/2011. http://www.ema.europa.eu/docs/en_GB/document_library/Scientific_guideline/2013/10/WC500152653.pdf Google Scholar
De Haas, Y, Barkema, W & Veerkamp, RF 2002 The effect of pathogen-specific clinical mastitis on the lactation curve for somatic cell count. Journal of Dairy Science 85 1314–1323 CrossRefGoogle ScholarPubMed
De Haas, Y, Veerkamp, RF, Barkema, HW, Gröhn, YT & Schukken, YH 2004 Associations between pathogen-specific cases of clinical mastitis and somatic cell count patterns. Journal of Dairy Science 87 95–105 Google Scholar
Deluyker, HA, Van Oye, SN & Boucher, JF 2005 Factors affecting cure and somatic cell count after pirlimycin treatment of subclinical mastitis in lactating cows. Journal of Dairy Science 88 604–614 Google Scholar
Dogan, B, Klaessig, S, Rishniw, M, Almeida, RA, Oliver, SP, Simpson, K & Schukken, YH 2006 Adherent and invasive Escherichia coli are associated with persistent bovine mastitis. Veterinary Microbiology 116 270–282 CrossRefGoogle ScholarPubMed
Döpfer, D, Barkema, HW, Lam, TJGM, Schukken, YH & Gaastra, W 1999 Recurrent clinical mastitis caused by Escherichia coli in dairy cows. Journal of Dairy Science 82 80–85 CrossRefGoogle ScholarPubMed
German Veterinary Association (GVA) 2009 Guidelines for aseptic sampling, isolation and identification of mastitis pathogens Fachgruppe Milchhygiene, Sachverständigenausschuss, Subklinische Mastitis, DVG, GerßenGoogle Scholar
Gillespie, BE & Oliver, SP 2004 Comparison of an automated ribotyping system, pulsed-field gel electrophoresis and randomly amplified polymorphic DNA fingerprinting for differentiation of Streptococcus uberis . Strains Biotechnology 3 165–172 Google Scholar
Gillespie, BE, Jayarao, BM & Oliver, SP 1997 Identification of Streptococcus species by randomly amplified polymorphic deoxyribonucleic acid fingerprinting. Journal of Dairy Science 80 471–476 CrossRefGoogle ScholarPubMed
Gillespie, BE, Moorehead, H, Lunn, P, Dowlen, HH, Johnson, DL, Lamar, KC, Lewis, MJ, Ivey, SJ, Hallberg, JW, Chester, ST & Oliver, SP 2002 Efficacy of extended pirlimycin hydrochloride therapy for treatment of environmental Streptococcus spp and Staphylococcus aureus intramammary infections in lactating dairy cows. Veterinary Therapeutics 3 373–380 Google ScholarPubMed
Halasa, T 2012 Bioeconomic modeling of intervention against clinical mastitis caused by contagious pathogens. Journal of Dairy Science 95 5740–5749 CrossRefGoogle ScholarPubMed
Halasa, T, Nielen, M, Huirne, RBM & Hogeveen, H 2009 Stochastic bio-economic model of bovine intramammary infection. Livestock Science 124 295–305 CrossRefGoogle Scholar
Hensen, SM, Pavicić, MJ, Lohuis, JA, de Hoog, JA & Poutrel, B 2000 Location of Staphylococcus aureus within the experimentally infected bovine udder and the expression of capsular polysaccharide type 5 in situ. Journal of Dairy Science 83 1966–1975 Google Scholar
Jarp, J, Bugge, HP & Larsen, S 1989 Clinical trial of three therapeutic regimens for bovine mastitis. Veterinary Record 124 630–634 CrossRefGoogle ScholarPubMed
Kerro Dego, O, van Dijk, JE & Nederbragt, H 2002 Factors involved in the early pathogenesis of bovine Staphylococcus aureus mastitis with emphasis on bacterial adhesion and invasion. A review. Veterinary Quarterly 24 181–198 CrossRefGoogle ScholarPubMed
Krömker, V 2013 Mastitis detection and prevention – what you can learn from clinical mastitis cases. RindSchweinSchaf 1 2–4 Google Scholar
Krömker, V, Paduch, JH, Klocke, D, Friedrich, J & Zinke, C 2010 Efficacy of extended intramammary therapy to treat moderate and severe clinical mastitis in lactating dairy cows. Berliner und Münchener tierärztliche Wochenschrift 123 147–152 Google Scholar
Lago, A, Godden, SM, Bey, R, Ruegg, PL & Leslie, K 2011a The selective treatment of clinical mastitis based on on-farm culture results: I. Effects on antibiotic use, milk withholding time, and short-term clinical and bacteriological outcomes. Journal of Dairy Science 94 4441–4456 CrossRefGoogle ScholarPubMed
Lago, A, Godden, SM, Bey, R & Leslie, K 2011b The selective treatment of clinical mastitis based on on-farm culture results: II. Effects on lactation performance, including clinical mastitis recurrence, somatic cell count, milk production, and cow survival. Journal of Dairy Science 94 4457–4467 CrossRefGoogle ScholarPubMed
Lam, TJGM 1996 Dynamics of Bovine mastitis. A field study in low somatic cell count herds. PhD Thesis, Faculty of Veterinary Medicine, Utrecht University Google Scholar
Milne, MH, Biggs, AM, Barrett, DC, Young, FJ, Doherty, S, Innocent, GT & Fitzpatrick, JL 2005 Treatment of persistent intramammary infections with Streptococcus uberis in dairy cows. Veterinary Record 157 245–250 Google Scholar
Mullarky, IK, Su, C, Frieze, N, Park, YH & Sordillo, LM 2001 Staphylococcus aureus agr genotypes with enterotoxin production capabilities can resist neutrophil bactericidal activity. Infection and Immunity 69 45–51 Google Scholar
Munoz, MA, Welcome, FL, Schukken, YH, Zadoks, RN 2007 Molecular epidemiology of two Klebsiella pneumoniae mastitis outbreaks on a dairy farm in New York State. Journal of Clinical Microbiology 45 3964–3971 CrossRefGoogle ScholarPubMed
Naffa, RG, Bdour, SM, Migdadi, HM, & Shehabi, AA 2006 Enterotoxicity and genetic variation among clinical Staphylococcus aureus isolates in Jordan. Journal of Medical Microbiology 55 183–187 CrossRefGoogle ScholarPubMed
National Mastitis Council (NMC) 1999 Laboratory Handbook on Bovine Mastitis. Madison, WI: National Mastitis Counc Inc.Google Scholar
Oliver, SP, Almeida, RA, Gillespie, BE, Headrick, SJ, Dowlen, HH, Johnson, DL, Lamar, KC, Chester, ST & Moseley, WM 2004a Extended ceftiofur therapy for treatment of experimentally-induced Streptococcus uberis mastitis in lactating dairy cattle. Journal of Dairy Science 87 3322–3329 Google Scholar
Oliver, SP, Gillespie, BE, Headrick, SJ, Moorehead, H, Lunn, P, Dowlen, HH, Johnson, DL, Lamar, KC, Chester, ST & Moseley, WM 2004b Efficacy of extended ceftiofur intramammary therapy for treatment of subclinical mastitis in lactating dairy cows. Journal of Dairy Science 87 2393–2400 Google Scholar
Pacheco, AB, Guth, BE, de Almeida, DF & Ferreira, LC 1996 Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA. Research in Microbiology 147 175–182 Google Scholar
Pyörälä, SHK & Pyörälä, EO 1998 Efficacy of parenteral administration of three antimicrobial agents in treatment of clinical mastitis; 487 cases (1989–1995). Journal of the American Veterinary Association 212 407–412 Google Scholar
Roy, J-P, DesCôteaux, L, DuTremblay, D, Beaudry, F, & Elsener, J 2009 Efficacy of a 5-day extended therapy program during lactation with cephapirin sodium in dairy cows chronically infected with Staphylococcus aureus . Canadian Veterinary Journal 50 1257–1262 Google ScholarPubMed
Schukken, YH, Bennett, GJ, Zurakowski, MJ, Sharkey, HL, Rauch, BJ, Thomas, MJ, Ceglowski, B, Saltman, RL, Belomestnykh, N & Zadoks, RN 2011 Randomized clinical trial to evaluate the efficacy of a 5-day ceftiofur hydrochloride intramammary treatment on nonsevere gram-negative clinical mastitis. Journal of Dairy Science 94 6203–6215 CrossRefGoogle ScholarPubMed
Sol, J, Sampimon, OC, Barkema, HW & Schukken, YH 2000 Factors associated with cure after therapy of clinical mastitis caused by Staphylococcus aureus . Journal of Dairy Science 83 278–284 Google Scholar
St Rose, SG, Swinkels, JM, Kremer, WD, Kruitwagen, CL & Zadoks, RN 2003 Effect of penethamate hydriodide treatment on bacteriological cure, somatic cell count and milk production of cows and quarters with chronic subclinical Streptococcus uberis or Streptococcus dysgalactiae infection. Journal of Dairy Research 70 387–394 CrossRefGoogle ScholarPubMed
Steeneveld, W, van Werven, T, Barkema, HW & Hogeveen, H 2011 Cow-specific treatment of clinical mastitis an economic approach. Journal of Dairy Science 94 174–188 CrossRefGoogle ScholarPubMed
Swinkels, JM, Cox, P, Schukken, YH & Lam, TJGM 2013a Efficacy of extended cefquinome treatment of clinical Staphylococcus aureus mastitis. Journal of Dairy Science 96 4983–4992 Google Scholar
Swinkels, JM, Lam, TJGM, Green, MJ & Bradley, AJ 2013b Effect of extended cefquinome treatment on clinical persistence or recurrence of environmental clinical mastitis. Veterinary Journal 197 682–687 Google Scholar
Truchetti, G, Bouchard, E, Descôteaux, L, Scholl, D & Roy, JP 2014 Efficacy of extended intramammary ceftiofur therapy against mild to moderate clinical mastitis in Holstein dairy cows: a randomized clinical trial. Canadian Journal of Veterinary Research 78 31–37 Google Scholar
Vogel, L, Jories, G, Tviep, S, Koek, A & Dijkshoorn, L 1999 RAPD typing of Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens and Pseudomonas aeruginosa isolates using standardized reagents. Clinical Microbiology and Infection 5 270–276 Google Scholar