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Effects of thermal industrial processing on acid-soluble nucleotides of milk

Published online by Cambridge University Press:  01 June 2009

Angel Gil  and
Fermin Sanchez-Medina
Angel Gil
Affiliation:
Departamento de Investigación de UNIASA, Granada, Spain
Fermin Sanchez-Medina
Affiliation:
Departamento Interfacultativo de Bioquímica, Universidad de Granada, Granada, Spain
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Summary

Commercial bulk milk contained, in its acid-soluble nucleotide fraction, small quantities of CMP and AMP as well as a high proportion of orotate (503±16 μg/g of total solids). These components were altered by thermal treatment during processing. Standard sterilization and spray drying of milk lowered significantly the CMP, AMP, and orotate concentrations. HTST pasteurization caused a slight decrease in CMP and AMP concentrations, but the orotate content remained unchanged. The UHT sterilization seemed to be the thermal treatment in which the acid-soluble nucleotides of milk underwent least change. It is therefore concluded that the decrease in concentration of the nucleotides of milk is more directly related to the time for which the milk remains at the processing temperature than to the temperature itself.

Type
Original Articles
Information
Journal of Dairy Research , Volume 49 , Issue 2 , May 1982 , pp. 295 - 300
Copyright
Copyright © Proprietors of Journal of Dairy Research 1982

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References

REFERENCES

Denamur, R., Fauconneau, G. & Guntz, G. 1959 [Acid soluble nucleotides in the milk of the ewe, cow, goat and sow]. Revista Española de Fisiologia 15 301–310Google ScholarPubMed
Empie, M. W. & Melachouris, N. 1977 Determination of orotic acid content in whey and modified whey products. Journal of Dairy Science 60 (Supplement 1) 48–49Google Scholar
Gil, A. & Sánchez-Medina, F. 1981 a Acid-soluble nucleotides of cow's, goat's, and sheep's milks, at different stages of lactation. Journal of Dairy Research 48 35–44CrossRefGoogle ScholarPubMed
Gil, A. & Sánchez-Medina, F. 1981 b The determination of acid-soluble nucleotides in milk by improved enzymic methods: a comparison with the ion-exchange column chromatography procedure. Journal of the Science of Food and Agriculture 32 1123–1131.Google Scholar
Hurlbert, R. B., Schmitz, H., Brumm, A. F. & Potter, V. R. 1954 Nucleotide metabolism. II. Chromatographic separation of acid-soluble nucleotides. Journal of Biological Chemistry 209 23–39CrossRefGoogle ScholarPubMed
Johke, T. & Goto, T. 1962 Acid-soluble nucleotides in cow's and goat's milk. Journal of Dairy Science 45 735–741CrossRefGoogle Scholar
Kobata, A., Ziro, S. & Kida, M. 1962 The acid-soluble nucleotides of milk. I. Quantitative and qualitative differences of nucleotides constituents in human and cow's milk. Journal of Biochemistry 51 277–287CrossRefGoogle Scholar
Larson, B. L. & Hegarty, H. M. 1979 Orotic acid in milks of various species and commercial dairy products. Journal of Dairy Science 62 1641–1644CrossRefGoogle Scholar
Möllering, H. 1974 Orotate: Determination with 0–5-MP pyrophosphorylase. In Methods of Enzymatic Analysis Vol. IV, 1959–1963, 2nd Edn (Ed. Bergmeyer, H. U.) New York: Academic PressCrossRefGoogle Scholar
Okonkwo, P. O. & Kinsella, J. E. 1969 a Orotic acid in food milk powders. American Journal of Clinical Nutrition 22 532–534CrossRefGoogle ScholarPubMed
Okonkwo, P. & Kinsella, J. E. 1969 b Orotic acid in yoghurt. Journal of Dairy Science 52 1861–1862CrossRefGoogle Scholar
Robinson, J. L. 1980 Bovine milk orotic acid: variability and significance for human nutrition. Journal of Dairy Science 63 865–871CrossRefGoogle Scholar
Rosenbloom, F. M. & Seegmiller, J. E. 1964 An enzymatic spectrophotometric method for determination of orotic acid. Journal of Laboratory and Clinical Medicine 63 492–500Google ScholarPubMed