Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T04:03:59.913Z Has data issue: false hasContentIssue false
  • English
  • Français

The effects of non-esterified fatty acids and β-hydroxybutyrate on the hepatic CYP2E1 in cows with clinical ketosis

Published online by Cambridge University Press:  08 February 2019

Zhicheng Peng ,
Xiaobing Li ,
Zhe Wang ,
Guowen Liu  and
Xinwei Li
Zhicheng Peng
Affiliation:
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Xiaobing Li
Affiliation:
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Zhe Wang
Affiliation:
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Guowen Liu
Affiliation:
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
Xinwei Li*
Affiliation:
Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China
*
Author for correspondence: Xinwei Li, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Dairy cows with ketosis display severe oxidative stress as well as high blood concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB). Cytochrome P4502E1 (CYP2E1) plays an important role in the induction of oxidative stress. The aim of this study was to investigate CYP2E1 expression and activity in the liver of clinically ketotic cows (in vivo) and the effects of NEFA and BHB on CYP2E1 expression and activity in hepatocytes (in vitro). Dairy cows with clinical ketosis exhibited a low blood concentration of glucose but high concentrations of NEFA and BHB. Hepatic mRNA, protein expression, and activity of CYP2E1 were significantly higher in cows with clinical ketosis than in control cows. In vitro, both NEFA and BHB treatment markedly up-regulated the mRNA and protein expressions as well as activity of CYP2E1 in cow hepatocytes. Taken together, these results indicate that high levels of NEFA and BHB significantly up-regulate the expression and activity of hepatic CYP2E1, and may be influential in the induction of oxidative stress in cows with clinical ketosis.

Keywords

CYP2E1ketosisoxidative stress
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 1 , February 2019 , pp. 68 - 72
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bai, J and Cederbaum, AI (2006) Adenovirus-mediated expression of CYP2E1 produces liver toxicity in mice. Toxicology Science 91, 365–371.Google Scholar
Butura, A, Nilsson, K, Morgan, K, Morgan, TR, French, SW, Johansson, I, Schuppe-Koistinen, I and Ingelman-Sundberg, M (2009) The impact of CYP2E1 on the development of alcoholic liver disease as studied in a transgenic mouse model. Journal of Hepatology 50, 572–583.Google Scholar
Cederbaum, AI 2010 Role of CYP2E1 in ethanol-induced oxidant stress, fatty liver and hepatotoxicity. Digestive Diseases 28 802–811.Google Scholar
Du, X, Chen, L, Huang, D, Peng, Z, Zhao, C, Zhang, Y, Zhu, Y, Wang, Z, Li, X and Liu, G (2017) Elevated apoptosis in the liver of dairy cows with ketosis. Cellular Physiology and Biochemistry 43, 568–578.Google Scholar
Du, X, Zhu, Y, Peng, Z, Cui, Y, Zhang, Q, Shi, Z, Guan, Y, Sha, X, Shen, T, Yang, Y, Li, X, Wang, Z, Li, X and Liu, G (2018) High concentrations of fatty acids and beta-hydroxybutyrate impair the growth hormone-mediated hepatic JAK2-STAT5 pathway in clinically ketotic cows. Journal of Dairy Science 101, 3476–3487.Google Scholar
Gross, J, van Dorland, HA, Bruckmaier, RM and Schwarz, FJ (2011) Performance and metabolic profile of dairy cows during a lactational and deliberately induced negative energy balance with subsequent realimentation. Journal of Dairy Science 94, 1820–1830.Google Scholar
Itle, AJ, Huzzey, JM, Weary, DM and von Keyserlingk, MAG (2015) Clinical ketosis and standing behavior in transition cows. Journal of Dairy Science 98, 128–134.Google Scholar
Jiang, Z, Wang, J, Xue, H, Wang, M, Jiang, H, Liang, Y, Dias, AC, Gregory, M, Chen, C and Zhang, X (2017) Protective effect of wild Corni fructus methanolic extract against acute alcoholic liver injury in mice. Redox Report 22, 338–345.Google Scholar
Leung, TM and Lu, Y (2017) Alcoholic liver disease: from CYP2E1 to CYP2A5. Current Molecular Pharmacology 10, 172–178.Google Scholar
Li, X, Huang, W, Gu, J, Du, X, Lei, L, Yuan, X, Sun, G, Wang, Z, Li, X and Liu, G (2015) SREBP-1c overactivates ROS-mediated hepatic NF-κB inflammatory pathway in dairy cows with fatty liver. Cellular Signalling 27, 2099–2109.Google Scholar
Li, Y, Ding, H, Wang, X, Feng, S, Li, X, Wang, Z, Liu, G and Li, X (2016 a) An association between the level of oxidative stress and the concentrations of NEFA and BHB in the plasma of ketotic dairy cows. Journal of Animal Physiology and Animal Nutrition 100, 844–851.Google Scholar
Li, Y, Ding, H, Wang, X, Liu, L, Huang, D, Zhang, R, Guo, L, Wang, Z, Li, X, Liu, G, Wu, J and Li, X (2016 b) High levels of acetoacetate and glucose increase expression of cytokines in bovine hepatocytes, through activation of the NF-κB signalling pathway. Journal of Dairy Research 83, 51–57.Google Scholar
McArt, JA, Nydam, DV, Oetzel, GR, Overton, TR and Ospina, PA (2013) Elevated non-esterified fatty acids and β-hydroxybutyrate and their association with transition dairy cow performance. The Veterinary Journal 198, 560–570.Google Scholar
Osabe, M, Sugatani, J, Fukuyama, T, Ikushiro, S, Ikari, A and Miwa, M (2008) Expression of hepatic UDP-glucuronosyltransferase 1A1 and 1A6 correlated with increased expression of the nuclear constitutive androstane receptor and peroxisome proliferator-activated receptor alpha in male rats fed a high-fat and high sucrose diet. Drug Metabolism and Disposition 36, 294–302.Google Scholar
Schieber, M and Chandel, NS (2014) ROS function in redox signaling and oxidative stress. Current Biology 24, 453–462.Google Scholar
Shi, X, Li, D, Deng, Q, Li, Y, Sun, G, Yuan, X, Song, Y, Wang, Z, Li, X, Li, X and Liu, G (2015) NEFAs activate the oxidative stress-mediated NF-kB signaling pathway to induce inflammatory response in calf hepatocytes. Journal of Steroid Biochemistry and Molecular Biology 145, 103–112.Google Scholar
Song, Y, Li, X, Li, Y, Li, N, Shi, X, Ding, H, Zhang, Y, Li, X, Liu, G and Wang, Z (2014) Non-esterified fatty acids activate the ROS–p38–p53/Nrf2 signaling pathway to induce bovine hepatocyte apoptosis in vitro. Apoptosis 19, 984–997.Google Scholar
Song, Y, Li, N, Gu, J, Fu, S, Peng, Z, Zhao, C, Zhang, Y, Li, X, Wang, Z, Li, X and Liu, G (2016) β-Hydroxybutyrate induces bovine hepatocyte apoptosis via an ROS-p38 signaling pathway. Journal of Dairy Science 99, 9184–9198.Google Scholar
Supplementary material: PDF

Peng et al. supplementary material

Peng et al. supplementary material 1

Download Peng et al. supplementary material(PDF)
PDF 239.8 KB