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Effects of herd and physiological status on variation of 16 immunological and inflammatory parameters in dairy cows during drying off and the transition period

Published online by Cambridge University Press:  22 May 2018

Alfonso Zecconi ,
Francesca Albonico ,
Maria Elena Gelain ,
Renata Piccinini ,
Micaela Cipolla  and
Michele Mortarino
Alfonso Zecconi*
Affiliation:
Department of Veterinary Medicine, University of Milan, via Celoria 10, 20133 Milan, Italy
Francesca Albonico
Affiliation:
Department of Veterinary Medicine, University of Milan, via Celoria 10, 20133 Milan, Italy
Maria Elena Gelain
Affiliation:
Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020, Agripolis, Legnaro (PD), Italy
Renata Piccinini
Affiliation:
Department of Veterinary Medicine, University of Milan, via Celoria 10, 20133 Milan, Italy
Micaela Cipolla
Affiliation:
Department of Veterinary Medicine, University of Milan, via Celoria 10, 20133 Milan, Italy
Michele Mortarino
Affiliation:
Department of Veterinary Medicine, University of Milan, via Celoria 10, 20133 Milan, Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

During drying off and transition period, cows are subject to changes in endocrine status, metabolic stressors and altered immune functions, which could lead to an increased risk of disease. To expand our knowledge on the immune/inflammatory status and to identify markers to define cow status during this interval, the pattern of 9 different cellular parameters, 5 cytokines, 2 enzymes and 3 cellular ratios in blood samples were assessed in 15 primiparous cows belonging to three different dairy herds in Lombardy. Our data showed that the variation of almost all parameters was influenced by the physiological period in which the samples were collected, except for apoptosis, IL-1β, IL-6, lysozyme and granulocyte/monocyte ratio. Several markers were directly correlated either to the herd alone (IL-1β, IL-6, lysozyme, granulocyte/lymphocyte ratio and granulocyte/monocyte ratio) or in association with the sampling time (white blood cell count, necrosis, lymphocytes count, CD4+ lymphocytes proportion). Hierarchical cluster analysis identified three herd-associated sample clusters showing different frequency along the follow-up period. The results of this field study highlight the importance of the herd factor in the immune/inflammatory response. Furthermore, these results suggest that cellular parameters are probably the most suitable markers to define cow status during drying-off and the peripartum period.

Keywords

Dairy cowstransition perioddrying offimmunitymarkers
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 167 - 173
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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