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Effect of yogurt feeding on the small and large intestine associated lymphoid cells in mice

Published online by Cambridge University Press:  01 June 2009

Gabriela Perdigón ,
Mirta Rachid ,
Marta V. De Budeguer  and
Juan C. Valdez
Gabriela Perdigón*
Affiliation:
Sección Inmunología, Institute de Microbiología, Facultad de Bioquímica, Química y Farmatia, Universidad Nacional de Tucumán, Ayacucho 491, Tucumán, Argentina Centra de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000 San Miguel de Tucumá, Argentina
Mirta Rachid
Affiliation:
Sección Inmunología, Institute de Microbiología, Facultad de Bioquímica, Química y Farmatia, Universidad Nacional de Tucumán, Ayacucho 491, Tucumán, Argentina
Marta V. De Budeguer
Affiliation:
Facultad de Medicina, Universidad Nacional de Tucumán, Tucumán, Argentina
Juan C. Valdez
Affiliation:
Sección Inmunología, Institute de Microbiología, Facultad de Bioquímica, Química y Farmatia, Universidad Nacional de Tucumán, Ayacucho 491, Tucumán, Argentina
*
‡ For correspondence.
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Summary

The effect of giving yogurt supplements to Balb/c mice on the various gut-associated lymphoid cells was studied. Animals were fed for 2, 5, 7 and 10 consecutive days. The different lymphoid cell types were identified and counted by haematoxylin–eosin staining of histological slices. The numbers of cells secreting IgA, IgG and IgM and the numbers of T lymphocytes were determined by direct immunofluorescence. The degree of activation of the intestinal macrophages in the small intestine was assessed by measuring the β-glucuronidase (EC 3.2.1.31) released into the intestinal fluid, and also by a histochemical method. Throughout the feeding period, there were no histological alterations in the gut, but there was marked cell infiltration, mainly of plasma cells and lymphocytes. The number of macrophages on the small intestine increased significantly after feeding for 2 d, while the β-glucuronidase activity was only slightly higher that of the controls. After a 7 d feeding period, the number of IgA secreting cells increased, while the values for cells secreting IgM and IgG and for T lymphocytes remained similar to those of the controls. The effect of giving yogurt on lymphoid cells associated with the large intestine was mainly on the numbers of IgA secreting B cells and T lymphocytes, with a marked increase during the whole feeding period in the latter type of cell. Since giving yogurt mainly enhanced the IgA secreting B cells in both small and large intestines, this increase would strengthen the host's defence mechanisms in the intestinal mucosa. Although the number of macrophages was increased, there was no enhancement in their activity, which might have harmed the host by producing an inflammatory response.

Type
Original articles
Information
Journal of Dairy Research , Volume 61 , Issue 4 , November 1994 , pp. 553 - 562
Copyright
Copyright © Proprietors of Journal of Dairy Research 1994

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