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Effect of sunflower-seed oil or linseed oil on milk fatty acid secretion and lipogenic gene expression in goats fed hay-based diets

Published online by Cambridge University Press:  13 March 2009

Laurence Bernard ,
Christine Leroux ,
Yannick Faulconnier ,
Denys Durand ,
Kevin J Shingfield  and
Yves Chilliard
Laurence Bernard*
Affiliation:
INRA, UR1213 Herbivores, Equipe Tissu Adipeux et Lipides du Lait, Site de Theix, F-63122 Saint-Genès-Champanelle, France
Christine Leroux
Affiliation:
INRA, UR1213 Herbivores, Equipe Tissu Adipeux et Lipides du Lait, Site de Theix, F-63122 Saint-Genès-Champanelle, France
Yannick Faulconnier
Affiliation:
INRA, UR1213 Herbivores, Equipe Tissu Adipeux et Lipides du Lait, Site de Theix, F-63122 Saint-Genès-Champanelle, France
Denys Durand
Affiliation:
INRA, UR1213 Herbivores, Equipe Tissu Adipeux et Lipides du Lait, Site de Theix, F-63122 Saint-Genès-Champanelle, France
Kevin J Shingfield
Affiliation:
Animal Production Research, MTT Agrifood Research Finland, Jokioinen, FIN 31600, Finland
Yves Chilliard
Affiliation:
INRA, UR1213 Herbivores, Equipe Tissu Adipeux et Lipides du Lait, Site de Theix, F-63122 Saint-Genès-Champanelle, France
*
*For correspondence; e-mail: [email protected]
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Abstract

Plant oils in the diet are known to alter milk fat composition owing to changes in the supply of fatty acid precursors and/or activity of lipogenic enzymes in the mammary gland. Thirteen mid-lactating Alpine goats were used in a 3×3 Latin square design with 28-d periods to evaluate possible mechanisms regulating milk fat synthesis and fatty acid composition on grass hay-based diets containing none (H) or 55 g/kg diet dry matter of sunflower-seed oil (HSO) or linseed oil (HLO). Inclusion of oils in the diet had no effect on milk yield but enhanced (P<0·05) milk fat secretion. Compared with the control, HLO and HSO decreased (P<0·05) C10–C16 secretion and increased (P<0·05) C18 output in milk, responses that were accompanied by reductions in milk fat cis-9 14:1/14:0, cis-9 18:1/18:0 and cis-9, trans-11 18:2/cis-9 18:1 concentration ratios. Plant oil supplements decreased (P<0·05) mammary stearoyl-CoA desaturase (SCD) activity but had no effect on SCD mRNA. Treatments had no effect on glucose-6-phosphate dehydrogenase, malic enzyme and glycerol-3-phosphate dehydrogenase activity, or mRNA abundance and/or activity of lipoprotein lipase, acetyl-CoA carboxylase and fatty acid synthase in mammary, hepatic or adipose tissue. The results provided little support for milk fatty acid secretion responses to HLO and HSO being mediated via changes in mammary, hepatic or adipose mRNA abundance or in the activity of key lipogenic enzymes. In conclusion, plant oils in the diet enhance milk fat synthesis, alter milk fatty acid composition and specifically inhibit mammary SCD activity in the goat. Furthermore, the results suggest that the regulation of mammary lipogenesis in response to plant oils appears related to factors other than altered mammary gene expression or potential lipogenic enzyme activity.

Keywords

Plant oilslipogenesisgene expressionenzyme activitylactating goat
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 241 - 248
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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