Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T05:36:24.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of different oxygen concentrations in the gas phase on biochemical activities of goat mammary tissue in organ culture

Published online by Cambridge University Press:  01 June 2009

Josef Škarda  and
Eva Urbanová
Josef Škarda
Affiliation:
Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences, 104 00 Prague, Czechoslovakia
Eva Urbanová
Affiliation:
Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences, 104 00 Prague, Czechoslovakia
Get access Rights & Permissions [Opens in a new window]

Summary

Non-secretory mammary expiants from virgin goats showed higher RNA and protein synthesis in a low O2 gas phase (air) than in high O2 (95% O2). Lipid and casein synthesis was not affected significantly by the concentration of O2 in the atmosphere during culture. on the other hand, the more developed mammary tissue from primigravid goats showed higher lipid, casein and protein synthesis in 95% O2. The relative response of mammary tissue to hormones was not substantially different when cultured in the presence of a low or high O2 gas phase. As Hepes-buffered medium was found not to need a supply of CO2 to maintain the correct pH and as Hepes did not interfere with biochemical activities of cells, it is recommended to use it for cultures in a low O2 gas phase.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 1 , February 1989 , pp. 1 - 5
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allison, A. C. 1968 Lysozomes. In The Biological Basis of Medicine, Vol. 1, p. 218 (Eds Bittar, E. E. & Bittar, N.). London: Academic PressGoogle Scholar
Barnawell, E. B. 1965. A comparative study of the responses of mammary tissues from several mammalian species to hormones in vitro. Journal of Experimental Zoology 160 189–206CrossRefGoogle ScholarPubMed
Brady, R. O. 1958. The enzymatic synthesis of fatty acids by aldol condensation. Proceedings of the National Academy of Sciences of the United States of America 44 993–998CrossRefGoogle ScholarPubMed
Delouis, C. & Combaud, M.-L. 1977. Lack of mitotic effects of insulin during synthesis of casein induced by prolactin in pseudopregnant rabbit mammary gland organ cultures. Journal of Endocrinology 72 393–394CrossRefGoogle ScholarPubMed
Dils, R. & Forsyth, I. A. 1981. Preparation and culture of mammary gland expiants. Methods in Enzymology 72 724–742CrossRefGoogle Scholar
Elias, J. J. 1957 Cultivation of adult mouse mammary gland in hormone-enriched synthetic medium. Science 128 842–844CrossRefGoogle Scholar
Fisk, A. & Pathak, S. 1969. HEPES-buffered medium for organ culture. Nature 224 1030–1031CrossRefGoogle ScholarPubMed
Forsyth, I. A. & Myres, R. P. 1971. Human prolactin. Evidence obtained by the bioassay of human plasma. Journal of Endocrinology 51 157–168CrossRefGoogle ScholarPubMed
Forsyth, I. A. & Turvey, A. 1984. Fatty acid synthesis by expiant cultures from the mammary glands of goats on days 60 and 120 of pregnancy. Journal of Endocrinology 100 87–92CrossRefGoogle Scholar
Gertler, A., Weil, A. & Cohen, N. 1982 Hormonal control of casein synthesis in organ culture of the bovine lactating mammary gland. Journal of Dairy Research 49 387–398CrossRefGoogle ScholarPubMed
Juergens, W. G., Stockdale, F. E., Topper, Y. J. & Elias, J. J. 1965 Hormone-dependent differentiation of mammary gland in vitro. Proceedings of the National Academy of Sciences of the United States of America 54 629–634CrossRefGoogle ScholarPubMed
Mayne, R. & Barry, J. M. 1970 Biochemical changes during development of mouse mammary tissue in organ culture. Journal of Endocrinology 46 61–70CrossRefGoogle ScholarPubMed
Prop, F. J. A. & Hendrix, S. E. A. M. 1965. Effect of insulin on mitotic rate in organ cultures of total mammary glands of the mouse. Experimental Cell Research 40 277–281CrossRefGoogle ScholarPubMed
Schingoethe, D. J., Hageman, E. C. & Larson, B. L. 1967 Essential amino acids for milk protein synthesis in the in vitro secretory cell and stimulation by elevated levels. Biochimica et Biophysica. Acta 148 469–474CrossRefGoogle ScholarPubMed
Škarda, J. & Bílek, J. 1975 Response of mammary tissue from pregnant goats to prolactin and growth hormone in organ culture. Journal of Endocrinology 67 129–130CrossRefGoogle ScholarPubMed
Škarda, J., Bílek, J. & Urbanová, E. 1978 a Induction of lipid synthesis in mammary organ cultures from mature virgin and pregnant goats. Physiologia Bohemoslovaca 27 53–59Google ScholarPubMed
Škarda, J., Urbanová, E. & Bílek, J. 1978 b Changes in insulin and prolactin responsiveness of goat mammary tissue during ontogenesis and pregnancy. Endocrinologia Experimentalis 12 217–232Google ScholarPubMed
Škarda, J., Urbanová, E., Houdebine, L.-M., Delouis, C. & Bílek, J. 1982 a Effects of insulin, cortisol and prolactin on lipid, protein and casein syntheses in goat mammary tissue in organ culture. Reproduction, Nutrition, Développement 22 379–386Google ScholarPubMed
Škarda, J., Urbanová, E. & Bílek, J. 1982 b Effects of progesterone, triiodothyronine and metabolic inhibitors on prolactin stimulated lipid synthesis in goat mammary expiants. Endocrinologia Experimentalis 16 81–92Google Scholar
Snedecor, G. W. 1956 Statistical Methods, 5th edn.Ames, IA: Iowa State University PressGoogle Scholar
Trowell, O. A. 1959 The culture of mature organs in a synthetic medium. Experimental Cell Research 16 118–147CrossRefGoogle Scholar
Warner, M. R. & Medina, D. 1975 Gas contaminant inhibition of mammary gland differentiation in vitro. Journal of the National Cancer Institute 54 1257–1258CrossRefGoogle ScholarPubMed
Waymouth, C. 1959 Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1). Journal of the National Cancer Institute 22 1003–1017CrossRefGoogle Scholar