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Effect of combined ultrasonic and heat treatment (thermoultrasonication) on the survival of a strain of Staphylococcus aureus

Published online by Cambridge University Press:  01 June 2009

Juan A. Ordoñez
Affiliation:
Departamento de Higiene y Microbiología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040-Madrid, Spain
M. Angeles Aguilera
Affiliation:
Departamento de Higiene y Microbiología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040-Madrid, Spain
M. Luisa Garcia
Affiliation:
Departamento de Higiene y Microbiología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040-Madrid, Spain
Bernabe Sanz
Affiliation:
Departamento de Higiene y Microbiología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040-Madrid, Spain

Summary

The combined effect of ultrasonic waves (20 kHz, 150 W) and heat treatment applied simultaneously (thermoultrasonication) on the survival of a strain of Staphylococcus aureus was studied in 0·05 m-phosphate buffer pH 6·8 and ultra-heat-treated whole milk. This combined process decreased by 63% the decimal reduction times for the heat treatment when the organism was suspended in buffer and by 43% when suspended in milk. These effects were much greater than the additive effect of the two agents considered independently.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

REFERENCES

Ahmed, F. I. K. & Russell, C. 1975 Synergism between ultrasonic waves and hydrogen peroxide in the killing of micro-organisms. Journal of Applied Bacteriology 39 3140CrossRefGoogle ScholarPubMed
Alliger, H. 1975 Ultrasonic disruption. American Laboratory 10 7585Google Scholar
Berliner, S. 1984 Application of ultrasonic processors. International Biotechnology Laboratory 2 4249Google Scholar
Beuchat, L. R. & Lechowich, R. V. 1968 Effect of salt concentration in the recovery medium on heat-injured Streptococcus faecalis. Applied Microbiology 16 772776CrossRefGoogle ScholarPubMed
Boucher, R. M. G. 1979 Ultrasonics: a tool to improve biocidal efficacy of sterilants or disinfectants in hospital and dental practice. Canadian Journal of Pharmaceutical Sciences 14 112Google Scholar
Burger, M. & Winder, W. C. 1954 Homogenization and deaeration of milk by ultrasonic waves. Journal of Dairy Science 37 645Google Scholar
Davies, R. 1959 Observations on the use of ultrasound waves for the disruption of microorganisms. Biochimica et Biophysica Acta 33 481493CrossRefGoogle ScholarPubMed
Garcia, M. L. 1985 [Effect of ultrasonic and heat treatments on Bacillus subtilis spores.] Doctoral Thesis, Universidad Complutense de Madrid.Google Scholar
Hughes, D. E. & Nyborg, W. L. 1962 Cell disruption by ultrasound. Science 138 108114CrossRefGoogle ScholarPubMed
Hurst, A., Hughes, A. & Collins-Thompson, D. L. 1974 The effect of sublethal heating on Staphylococcus aureus at different physiological ages. Canadian Journal of Microbiology 20 765768CrossRefGoogle ScholarPubMed
Kinsloe, H., Ackerman, E. & Reid, J. J. 1954 Exposure of microorganisms to measured sound fields. Journal of Bacteriology 68 373380CrossRefGoogle ScholarPubMed
Ordoñez, J. A., Sanz, B., Hernandez, P. E. & Lopez-Lorenzo, P. 1984 A note on the effect of combined ultrasonic and heat treatments on the survival of thermoduric streptococci. Journal of Applied Bacteriology 56 175177CrossRefGoogle ScholarPubMed
Paci, C. 1953 [Use of ultrasound to sterilize milk.] Lait 33 610615CrossRefGoogle Scholar
Sanz Perez, B., Lopez Lorenzo, P., Garcia, M. L., Hernandez, P. E. & Ordoñez, J. A. 1982 Heat resistance of enterococci. Milchwissenschaft 37 724726Google Scholar
Shaner, E. O. 1964 Augmentation of bacterial action of germicides with ultrasound. Journal of the Acoustics Society of America 36 22382239CrossRefGoogle Scholar
Taylor, M. J. & Richardson, T. 1980 Antioxidant activity of skim milk: effect of sonication. Journal of Dairy Science 63 19381942CrossRefGoogle ScholarPubMed
Thomas, C. E., White, J. C. & Karla, L. 1966 Thermal resistance of salmonellae and staphylococci in foods. Applied Microbiology 14 815820CrossRefGoogle ScholarPubMed
Utsunomiya, Y. & Kosaka, Y. 1979 [Application of supersonic waves to foods.] Journal of the Faculty of Applied Biological Science, Hiroshima University 18 225231Google Scholar