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Effect of a biological response modifier on cellular death mechanisms at drying off

Published online by Cambridge University Press:  12 May 2008

Bibliana E Dallard ,
Celina Baravalle ,
Hugo Ortega ,
Valeria Ruffino ,
Silvia Heffel  and
Luis F Calvinho
Bibliana E Dallard
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Celina Baravalle
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Hugo Ortega
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Valeria Ruffino
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Silvia Heffel
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Luis F Calvinho
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, C.C. 22 (2300) Rafaela, Santa Fe, Argentina
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Abstract

Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objective of this study was to describe the effects of a single intramammary infusion of a lipopolysaccharide (LPS)-based biological response modifier (BRM) on cellular death mechanism in uninfected and Staphylococcus aureus-infected bovine mammary glands during involution. Three groups of 12 cows, each one including 6 Staph. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax at every observation period. In addition, BRM produced a significant increase of immunostained stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of involution compared with placebo-treated quarters and a significant decrease in percentages of terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3 and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining, was increased in epithelial and stromal cells from Staph. aureus-infected compared with uninfected quarters at every observation period. These results provide new insights into the mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell proliferation during mammary involution.

Keywords

Biological response modifiermammary gland involutionStaphylococcus aureusapoptosis
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 2 , May 2008 , pp. 167 - 175
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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