Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-25T03:14:23.608Z Has data issue: false hasContentIssue false
  • English
  • Français

The dynamics of individual whey protein concentrations in cows’ mammary secretions during the colostral and early lactation periods

Published online by Cambridge University Press:  06 December 2018

Raquel F.S. Raimondo ,
Juliana S.P. Ferrão ,
Samantha I. Miyashiro ,
Priscila T. Ferreira ,
João Paulo E. Saut ,
Daniela B. Birgel  and
Eduardo H. Birgel Junior
Raquel F.S. Raimondo*
Affiliation:
Núcleo RuminAção, ensino, pesquisa e extensão em ruminantes. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, 90040-060, Brazil
Juliana S.P. Ferrão
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Sao Paulo, Brazil
Samantha I. Miyashiro
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Sao Paulo, Brazil
Priscila T. Ferreira
Affiliation:
Núcleo RuminAção, ensino, pesquisa e extensão em ruminantes. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, 90040-060, Brazil
João Paulo E. Saut
Affiliation:
Large Animal Health Laboratory, Faculty of Veterinary Medicine, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
Daniela B. Birgel
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Sao Paulo, Brazil
Eduardo H. Birgel Junior
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Sao Paulo, Brazil
*
Authors for correspondence: Raquel F.S. Raimondo, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The bovine whey consists of more than 200 different types of proteins, of which β-lactoglobulin, α-lactalbumin, serum albumin, immunoglobulins and lactoferrin predominate. However, their concentrations are not stable due to the existence of protein dynamics during a transition from colostrum secretion to mature milk. To evaluate the dynamics of whey proteins of Jersey cows during a colostral phase and first month of lactation and an influence of the number of lactations, 268 milk samples from 135 Jersey cows were selected through a clinical evaluation. Whey was obtained by rennet coagulation of the mammary secretion. The concentration of total proteins was determined by the biuret method and their fractions were identified by 12% dodecyl sulfate-polyacrylamide gel electrophoresis (12% SDS-PAGE). Maximum concentrations of all protein fractions were observed in the first 12 h of lactation, reducing over the course of the study. Modification of the protein predominance was also observed. The transition from colostrum secretion to milk occurred between 24 and 72 h postpartum. There was an influence of the number of lactations on the dynamics of whey proteins, indicating that multiparous cows had better immunological and nutritional quality when compared to primiparous cows.

Keywords

Alpha-lactalbumincolostrumimmunoglobulinSDS-PAGEwhey
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 1 , February 2019 , pp. 88 - 93
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chigerwe, M, Dawes, ME, Tyler, JW, Middleton, JR, Moore, MP and Nagy, DM (2005) Evaluation of a cow-side immunoassay kit for assessing IgG concentration in colostrum. Journal of American Veterinary Medical Association 227, 129–131.Google Scholar
Costa, EO, Melville, PA, Ribeiro, AR, Watanabe, E, Viani, FC and White, CR (1996) Prevalence of intramammary infections in primigravid Brazilian dairy heifers. Preventive Veterinary Medicine 29, 151–155.Google Scholar
Dirksen, G, Gründer, H-D and Stöber, M (1993) Rosenberg Die Klinische Untersuchung des Rindes. 3rd ed. Berlin and Hamburg: Verlagbuchhandlung Paul Parey.Google Scholar
Guilloteau, P, Romé, V, Delaby, L, Mendy, F, Roger, L and Chayvialle, JA (2009) A new role of phosphopeptides as bioactive peptides released during milk casein digestion in the young mammal: regulation of gastric secretion. Peptides 30, 2221–2227.Google Scholar
Heng, GB (1999) Chemical composition of bovine colostrums. Pages 405–411 in International Conference of Food Science and Technology. Anais, Trumbull, CT Trumbull: Food and Nutrition Press.Google Scholar
Hillier, RM (1976) The quantitative measurement of whey proteins using polyacrylamide-gel electrophoresis. Journal of Dairy Research 43, 259–265.Google Scholar
Hiss, S, Meyer, T and Sauerwein, H (2008) Lactoferrin concentrations in goat milk throughout lactation. Small Ruminant Research 80, 87–90.Google Scholar
Króls, J, Brodziak, A, Litwińczuk, Z and Litwińczuk, A (2013) Effect of age and stage of lactation on whey protein content in milk of cows of different breeds. Polish Journal of Veterinary Science 16, 395–397.Google Scholar
Levieux, D and Ollier, A (1999) Bovine immunoglobulin G, beta-lactoglobulin, alpha-lactalbumin and serum albumin in colostrum and milk during the early post partum period. Journal of Dairy Research 66, 421–430.Google Scholar
Madsen, BD, Rasmussen, MD, Nielsen, MO, Wiking, L and Larsen, LB (2004) Physical properties of mammary secretions in relation to chemical changes during transition from colostrum to milk. Journal of Dairy Research 71, 263–272.Google Scholar
McGrath, BA, Fox, PF, Mcsweeney, PLH and Kelly, L (2016) Composition and properties of bovine colostrum: a review. Dairy Science Technology 96, 133–158.Google Scholar
Nguyen, DD, Neville, MC (1998) Tight junction regulation in the mammary gland. Journal of Mammary Gland Biology and Neoplasia 3, 233–246.Google Scholar
Phillippy, BO, Mccarthy, RD (1979) Multi-origins of milk serum albumin in the lactating goat. Biochimica Biophysica Acta 584, 298–303.Google Scholar
Raimondo, RFS, Mori, CS, Miyiashiro, SI and Junior, EHB (2010 a) Standardization of the Biuret Method for determining the whey proteins. Page 119 in World Buiatrics Congress. Proc., Santiago. Santiago: World Association for Buiatrics.Google Scholar
Raimondo, RFS, Pogliani, FC, Birgel, DB, Saut, JPE and Birgel, EH Jr (2010 b) Whey protein profile from heifers and multiparous Jersey's cows during the early lactation. Page 254 in World Buiatrics Congress. Proc., Santiago. Santiago: World Association for Buiatrics.Google Scholar
Raimondo, RFS, Brandespim, FB, Prina, APM, Miyashiro, SI, Saut, JPE, Mori, CS, Pogliani, FC and Birgel, EH (2013 a) Dynamic in the concentration of whey proteins in the mammary secretion of goats during the dry period. Small Ruminant Research 113, 239–246.Google Scholar
Raimondo, RFS, Miyiashiro, SI, Mori, CS and Birgel Junior, EH (2013 b) Proteínas do soro lácteo de vacas da raça Jersey durante a lactação. Pesquisa Veterinária Brasileira 33, 119–125.Google Scholar
Senda, A, Fukuda, K, Ishii, T and Urashima, T (2011) Changes in the bovine whey proteome during the early lactation period. Animal Science Journal 82, 698–706.Google Scholar
Tenhagen, BA, Hansen, I, Reinecke, A and Heuwieser, W (2009) Prevalence of pathogens in milk samples of dairy cows with clinical mastitis and in heifers at first parturition. Journal of Dairy Research 76, 179–187.Google Scholar
Tsuji, S, Hirata, Y, Mukai, F and Ohtagaki, S (1990) Comparison of lactoferrin content in colostrum between different cattle breeds. Journal of Dairy Science 73, 125–128.Google Scholar
Yoshida, S, Wei, Z, Shinmura, Y and Fukunaga, N (2000) Separation of lactoferrin-a and -b from bovine colostrum. Journal of Dairy Science 83, 2211–2215.Google Scholar
Zhang, LY, Wang, JQ, Yang, YX, Bu, DP, Li, SS and Zhou, LY (2011) Comparative proteomic analysis of changes in the bovine whey proteome during the transition from colostrum to milk. Asian-australas. Journal of Animal Science 24, 272–278.Google Scholar