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Does β-lactoglobulin occur in human milk?

Published online by Cambridge University Press:  01 June 2009

Ghislaine Brignon ,
Abdessatar Chtourou  and
Bruno Ribadeau-Dumas
Ghislaine Brignon
Affiliation:
Laboratoire de Biochimie et Technologie Laitières, Institut National de la Recherche Agronomique, CNRZ, 78350 Jouy-en-Josas, France
Abdessatar Chtourou
Affiliation:
Laboratoire de Biochimie et Technologie Laitières, Institut National de la Recherche Agronomique, CNRZ, 78350 Jouy-en-Josas, France
Bruno Ribadeau-Dumas
Affiliation:
Laboratoire de Biochimie et Technologie Laitières, Institut National de la Recherche Agronomique, CNRZ, 78350 Jouy-en-Josas, France
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Summary

Although β-lactoglobulin (β-lg) has been considered to be absent from human milk, recent results of other workers, based on immunological reactions between human milk and rabbit antiserum to bovine β-lg, suggest that this protein may be present. Although our results show similar immunological reactions, we consider that lactoferrin is responsible for these, as it was the only reactive protein species which could be prepared to homogeneity. Indeed two types of antibodies were found by ELISA test in the antisera to bovine β-lg. One of them would be able to bind loosely to human lactoferrin, but its binding sites would not be antigenic in the rabbit.

Type
Original Articles
Information
Journal of Dairy Research , Volume 52 , Issue 2 , May 1985 , pp. 249 - 254
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

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References

REFERENCES

Aschaffenburg, R. & Drewry, J. 1957 Improved method for the preparation of crystalline β-laetoglobulin and α-lactalbumin from cow's milk. Biochemical Journal 65 273–277CrossRefGoogle ScholarPubMed
Brignon, G. & Ribadeau-Dumas, B. 1982 A new method for human milk protein separation. Biochimie 64 231–235CrossRefGoogle ScholarPubMed
Conti, A., Liberatori, J. & Napolitano, L. 1980 Isolation and preliminary physico-chemical characterization of human β-lactoglobulin. Milchwissenschaft 35 65–68Google Scholar
Conti, A., Napoitano, L. & Liberatori, J. 1982 Characterization of β-lactoglobulins from human colostrum. Some physico-chemical properties and preliminary amino acid evaluation. 21st International Dairy Congress, Moscow 1 (2) 163–164Google Scholar
Hekman, A. 1971 Association of lactoferrin with other proteins, as demonstrated by changes in electrophoretic mobility. Biochimica et Biophysica Acta 251 380–387CrossRefGoogle ScholarPubMed
Jenness, R. 1970 Protein composition of milk. In Milk Proteins. Chemistry and Molecular biology vol. I pp. 17–43 (Ed. McKenzie, H. A.) New York: Academic PressCrossRefGoogle Scholar
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Laurell, C. B. 1966 Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry 15 45–52CrossRefGoogle ScholarPubMed
Liberatori, J., Morisio-Guidetti, L. & Conti, A. 1979 Immunological evidence of β-lactoglobulins in human colostrum and milk. Bollettino delta Società ltaliana di Biologia Sperimentale 55 822–825Google ScholarPubMed
Moneret-Vautrin, A., Humbert, G., Alais, C. & Grilliat, J. P. 1982 [Recent data on the immunoallergic properties of dairy proteins] Lait 62 396–408CrossRefGoogle Scholar
Ruitenberg, E. J., Steerenberg, P. A., Brosi, B. J. M. & Buys, J. 1976 Reliability of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of Trichinella spiralis infections in conventionally raised pigs. Journal of Immunological Methods 10 67–83CrossRefGoogle ScholarPubMed
Scheidegger, J. J. 1955 [An Immunoelectrophoresis micro method] International Archives of Allergy and Applied Immunology 7 103–110CrossRefGoogle ScholarPubMed
Trayer, I. P. & Hill, R. L. 1971 The purification and properties of the A protein of laetose synthetase. Journal of Biological Chemistry 246 6666–6675CrossRefGoogle ScholarPubMed