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Diversity of yeasts and moulds in dairy products from Umbria, central Italy

Published online by Cambridge University Press:  14 May 2021

Beniamino Cenci-Goga ,
Deborah Cruciani ,
Silvia Crotti ,
Musafiri Karama ,
Gamze Yıldırım ,
Menekşe Bulut ,
Concetta Marino  and
Luca Grispoldi
Beniamino Cenci-Goga
Affiliation:
Medicina Veterinaria, Laboratorio di Ispezione degli Alimenti di Origine Animale, Università degli Studi di Perugia, 06126, Perugia, Italy Faculty of Veterinary Science, Department of Paraclinical Sciences, University of Pretoria, 0110, Onderstepoort, South Africa
Deborah Cruciani
Affiliation:
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche “Togo Rosati”, 06126, Perugia, Italy
Silvia Crotti
Affiliation:
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche “Togo Rosati”, 06126, Perugia, Italy
Musafiri Karama
Affiliation:
Faculty of Veterinary Science, Department of Paraclinical Sciences, University of Pretoria, 0110, Onderstepoort, South Africa
Gamze Yıldırım
Affiliation:
Engineering Faculty, Food Engineering Department, Afyon Kocatepe University, Afyon, Turkey
Menekşe Bulut
Affiliation:
Department of Food Engineering, Faculty of Engineering, Research Center for Redox Applications in Foods (RCRAF), Iğdır University, 76000, Iğdır, Turkey
Concetta Marino
Affiliation:
Medicina Veterinaria, Laboratorio di Ispezione degli Alimenti di Origine Animale, Università degli Studi di Perugia, 06126, Perugia, Italy
Luca Grispoldi*
Affiliation:
Medicina Veterinaria, Laboratorio di Ispezione degli Alimenti di Origine Animale, Università degli Studi di Perugia, 06126, Perugia, Italy
*
Author for correspondence: Luca Grispoldi, Email: [email protected]
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Abstract

In this research communication we report on the diversity of yeast and mould species in 69 samples of milk and different dairy products from three plants located in Umbria, central Italy. Isolates were characterised both macroscopically and microscopically and then identified by PCR and genome sequencing of the ITS region and the D1–D2 domain of the large-subunit rRNA gene for filamentous fungi and yeasts, respectively. Out of the 69 samples analysed, 51 (73.9%) tested positive for the presence of yeasts, whereas moulds were detected in 25 (36.2%) samples. A total of 9 yeast species belonging to 8 different genera and 13 mould species belonging to 6 different genera were isolated. The most common genera isolated were Debaryomyces and Kluyveromyces among the yeasts and Penicillium and Galactomyces among the moulds. Microbiota play a key role in the formation of flavour, aroma, texture and appearance of dairy products. This complex microbial ecosystem includes both cultured and external bacteria, yeasts and moulds. Some of them have an important role in the production of cheeses, whereas others are responsible for dairy product spoilage, resulting in significant food waste and economic losses. Some species can produce mycotoxins, representing a potential hazard for the consumer's safety. This study provides interesting information on the diversity of fungi species in dairy products from central Italy that can be of major importance to identify these products and to develop adequate strategies for fungal spoilage control and consumer safety.

Keywords

Dairy productsdiversitymouldsyeasts
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 2 , May 2021 , pp. 217 - 220
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Banjara, N, Suhr, MJ and Hallen-Adams, HE (2015) Diversity of yeast and mould species from a variety of cheese types. Current Microbiology 70, 1–9.CrossRefGoogle ScholarPubMed
Barrios, MJ, Medina, LM, Lopez, MC and Jordano, R (1998) Fungal biota isolated from Spanish cheeses. Journal of Food Safety 18, 151–157.10.1111/j.1745-4565.1998.tb00210.xCrossRefGoogle Scholar
Cenci-Goga, BT, Karama, M, Sechi, P, Iulietto, MF, Novelli, S and Mattei, S (2014) Evolution under different storage conditions of anomalous blue coloration of Mozzarella cheese intentionally contaminated with a pigment-producing strain of Pseudomonas fluorescens. Journal of Dairy Science 97, 6708–6718.CrossRefGoogle ScholarPubMed
Cholet, O, Henaut, A, Casaregola, S and Bonnarme, P (2007) Gene expression and biochemical analysis of cheese-ripening yeasts: focus on catabolism of L-methionine, lactate, and lactose. Applied and Environmental Microbiology 73, 2561–2570.CrossRefGoogle ScholarPubMed
Del Bove, M, Lattanzi, M, Rellini, P, Pelliccia, C, Fatichenti, F and Cardinali, G (2009) Comparison of molecular and metabolomic methods as characterization tools of Debaryomyces hansenii cheese isolates. Food Microbiology 26, 453–459.CrossRefGoogle ScholarPubMed
Dragoni, L, Papa, A and Vallone, L (1997) Mycological quality standards of Italian cheese Taleggio. Typical and unacceptable surface moulds – mycological paste standard [Lombardia]. Archivio Veterinario Italiano 48, 154–160.Google Scholar
Garnier, L, Valence, F, Pawtowski, A, Auhustsinava-Galerne, L, Frotté, N, Baroncelli, R, Deniel, F, Coton, E and Mounier, J (2017) Diversity of spoilage fungi associated with various French dairy products. International Journal of Food Microbiology 241, 191–197.CrossRefGoogle ScholarPubMed
Huis in't Veld, JHJ (1996) Microbial and biochemical spoilage of foods: an overview. International Journal of Food Microbiology 33, 1–18.CrossRefGoogle Scholar
Jacques, N and Casaregola, S (2008) Safety assessment of dairy microorganisms: the hemiascomycetous yeasts. International Journal of Food Microbiology 126, 321–326.CrossRefGoogle ScholarPubMed
Kandasamy, S, Park, WS, Yoo, J, Yun, J, Kang, HB, Seol, KH, Oh, MH and Ham, JS (2020) Characterisation of fungal contamination sources for use in quality management of cheese production farms in Korea. Asian-Australasian Journal of Animal Sciences 33, 1002–1011.CrossRefGoogle ScholarPubMed
Kure, C, Skaar, I and Brendehaug, J (2004) Mould contamination in production of semi-hard cheese. International Journal of Food Microbiology 93, 41–49.CrossRefGoogle ScholarPubMed
Ledenbach, LH and Marshall, RT (2010) Microbiological spoilage of dairy products. In Sperber, WH and Doyle, MP (eds), Compendium of the Microbiological Spoilage of Foods and Beverages. Food Microbiology and Food Safety. New York: Springer, pp. 41–67.Google Scholar
Montagna, MT, Santacroce, MP, Spilotros, G, Napoli, C, Minervini, F, Papa, A and Dragoni, I (2004) Investigation of fungal contamination in sheep and goat cheeses in southern Italy. Mycopathologia 158, 245–249.CrossRefGoogle ScholarPubMed
Mounier, J, Monnet, C, Vallaeys, T, Arditi, R, Sarthou, AS, Hélias, A and Irlinger, F (2008) Microbial interactions within a cheese microbial community. Applied and Environmental Microbiology 74, 172–181.CrossRefGoogle ScholarPubMed
Pitt, JI and Hocking, AD (2009) Fungi and Food Spoilage, 3rd Edn, New York, USA: Springer Science &Business Media.10.1007/978-0-387-92207-2CrossRefGoogle Scholar
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