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Development of an improved Streptococcus uberis experimental mastitis challenge model using different doses and strains in lactating dairy cows

Published online by Cambridge University Press:  20 July 2015

Manouchehr Khazandi ,
Patricia Eats ,
Darren Trott ,
Esmaeil Ebrahimie ,
Jeanette Perry ,
Elizabeth Hickey ,
Stephen Page ,
Sanjay Garg  and
Kiro R Petrovski
Manouchehr Khazandi
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Patricia Eats
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Darren Trott
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Esmaeil Ebrahimie
Affiliation:
Department of Genetics and Evolution, School of Biological Sciences, The University of Adelaide, South Australia, 5005, Australia Institute of Biotechnology, The University of Shiraz, Shiraz, Iran
Jeanette Perry
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Elizabeth Hickey
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Stephen Page
Affiliation:
Luoda Pharma Pty Ltd, Caringbah, New South Wales 2229, Australia
Sanjay Garg
Affiliation:
School of Pharmacy and Medical Science, The University of South Australia, South Australia 5000, Australia
Kiro R Petrovski*
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
*
*For correspondence; e-mail: [email protected]
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Abstract

Developing a reliable mastitis challenge infection model is required to test new intramammary antimicrobial preparations, other novel bovine mastitis treatments, and study mastitis pathogenesis. Three treatment groups of Holstein Friesian cows in active lactation were administered two doses (104 and 106 cfu/quarter) on a single occasion with one of the three Streptococcus uberis strains (BFR6019, MFF1283 and SA002) suspended in 5 ml of sterile PBS, administered via intramammary inoculation immediately after milking. All quarters that were challenged with S. uberis strains MLF1283 and BFR6019 showed clinical signs of mastitis on day 1 and 2 after the challenge. Strain SA002 had a lower rate of inducing clinical mastitis which was detected later than day 3 after the challenge. We successfully developed a rapid and reliable model for inducing experimental S. uberis mastitis with 100% success rate in cows in active lactation. On the basis of the correlation results between strains, RAPD fingerprinting results, clinical findings, and a 100% success rate of mastitis induction for low and high doses S. uberis strains MLF1283 and BFR6019, strain virulence seems to be a more important effect than challenge dose in induction of clinical mastitis following experimental challenge.

Keywords

Mastitischallenge modeldairy cowsStreptococcus uberis
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 4 , November 2015 , pp. 470 - 477
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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