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Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media

Published online by Cambridge University Press:  26 November 2009

Luís I M Souto ,
Clarice Y Minagawa ,
Evelise O Telles ,
Márcio A Garbuglio ,
Marcos Amaku ,
Priscilla A Melville ,
Ricardo A Dias ,
Sonia T Sakata  and
Nilson R Benites
Luís I M Souto*
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Clarice Y Minagawa
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Evelise O Telles
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Márcio A Garbuglio
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Marcos Amaku
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Priscilla A Melville
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Ricardo A Dias
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Sonia T Sakata
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Nilson R Benites
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
*
*For correspondence; e-mail: [email protected]
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Abstract

Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of one or more healthy animals. Mastitis is an inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk in four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were used: KF Streptococcus Agar, Edwards Agar, Baird-Parker Agar, Blood Agar plus potassium tellurite. Spearman's correlation coefficient was calculated in order to compare the occurrence of mastitis (percentage) in each herd with respective selective culture media counts of microorganisms in bulk raw milk. Thirty-six possibilities were analysed (Tamis and CMT-positive rates were compared with the log-transformed count in four selective culture media) and there was a negative correlation between Tamis 3 and the Baird-Parker Agar plate count. The total results of microbiological tests showed that there were three correlations of the counts in selective culture media. Fifty-two possibilities were analysed and there was a negative correlation between no-bacterial-growth mastitis rates and log10 of KF Streptoccocus Agar plate count and there were two positive correlations between coagulase-positive staphylococci and log10 of Baird-Parker Agar plate count and Blood Agar plus potassium tellurite plate count.

Keywords

Mastitisbulk raw milkselective culture media
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 1 , February 2010 , pp. 63 - 70
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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