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Compositional and electrophoretic changes in buffalo milk-fat globule membrane proteins during lactation

Published online by Cambridge University Press:  01 June 2009

Sukhminder Singh  and
N. C. Ganguli
Sukhminder Singh
Affiliation:
National Dairy Research Institute, Karnal-132001, India
N. C. Ganguli
Affiliation:
National Dairy Research Institute, Karnal-132001, India
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Summary

Chemical analyses, polyacrylamide-gel electrophoresis and isoelectric focusing of milk-fat globule membrane proteins (FGMP) obtained from the milk of 2 Murrah buffaloes were done to determine if any change in composition occurred during lactation. Changes in the levels of sialic acid, hexose, hexosamine, N and P were found in the FGMP obtained at different stages of lactation. On the day of parturition, 8 major proteins in FGMP were determined by sodium dodecyl sulphate polyacrylamide-gel electrophoresis whereas 6 major proteins were obtained in FGMP of middle and late lactation milks. Isoelectric focusing of FGMP showed 8–9, 9–13 and 13–16 proteins from colostrum, middle and late lactation milks, respectively and the isoelectric pH of the proteins varied from 5·25 to 7·80, 5·85 to 8·30 and 5·75 to 8·61 respectively.

Type
Original Articles
Information
Journal of Dairy Research , Volume 43 , Issue 3 , October 1976 , pp. 381 - 388
Copyright
Copyright © Proprietors of Journal of Dairy Research 1976

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