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Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk

Published online by Cambridge University Press:  27 February 2012

Katharina Hinz ,
Paula M O'Connor ,
Thom Huppertz ,
R Paul Ross  and
Alan L Kelly
Katharina Hinz
Affiliation:
School of Food and Nutritional Sciences, University College Cork, Cork, Ireland
Paula M O'Connor
Affiliation:
Teagasc Food Research Centre, Moorepark, Fermoy, Ireland
Thom Huppertz
Affiliation:
NIZO food research, Ede, The Netherlands
R Paul Ross
Affiliation:
Teagasc Food Research Centre, Moorepark, Fermoy, Ireland
Alan L Kelly*
Affiliation:
School of Food and Nutritional Sciences, University College Cork, Cork, Ireland
*
*For correspondence; e-mail: [email protected]
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Abstract

Proteomic analysis of bovine, caprine, buffalo, equine and camel milk highlighted significant interspecies differences. Camel milk was found to be devoid of β-lactoglobulin, whereas β-lactoglobulin was the major whey protein in bovine, buffalo, caprine, and equine milk. Five different isoforms of κ-casein were found in camel milk, analogous to the micro-heterogeneity observed for bovine κ-casein. Several spots observed in 2D-electrophoretograms of milk of all species could tentatively be identified as polypeptides arising from the enzymatic hydrolysis of caseins. The understanding gained from the proteomic comparison of these milks may be of relevance both in terms of identifying sources of hypoallergenic alternatives to bovine milk and detection of adulteration of milk samples and products.

Keywords

Milk proteinbovine milk protein allergy2-DEMALDI-ToF
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 2 , May 2012 , pp. 185 - 191
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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References

Ametani, A, Sakurai, T, Katakura, Y, Kuhara, S, Hirakawa, H, Hosoi, T, Dosako, S & Kaminogawa, S 2003 Amino acid residue substitution at T-cell determinant-flanking sites in beta-lactoglobulin modulates antigen presentation to T-cells through subtle conformational change. Bioscience, Biotechnology, Biochemistry 67 1507–1514CrossRefGoogle ScholarPubMed
Armaforte, E, Curran, E, Huppertz, T, Ryan, AC, Caboni, MF, O'Connor, PM, Ross, RP, Hirtz, C, Sommerer, N, Chevalier, F & Kelly, AL 2010 Proteins and proteolysis in pre-term and term human milk and possible implications for infant formulae. International Dairy Journal 20 715–723CrossRefGoogle Scholar
Baer, A, Ryba, I & Farah, Z 1994 Plasmin activity in camel milk. Lebensmittel Wissenschaft und Technologie 27 595–598CrossRefGoogle Scholar
Bevilacqua, C, Martin, P, Candalh, C, Fauquant, J, Piot, M, Roucayrol, AM, Pilla, F & Heyman, M 2001 Goat's milk of defective αs1-casein genotype decreases intestinal and systemic sensitization to β-lactoglobulin in guinea pigs. Journal of Dairy Research 68 217–227CrossRefGoogle Scholar
Buffoni, JN, Bonizzi, I, Paciullo, A, Ramunno, L & Feligini, M 2011 Characterization of the major whey proteins from milk of Mediterranean water buffalo (Bubalus bubalis). Food Chemistry 127 1515–1520CrossRefGoogle Scholar
Chin, HW & Rosenberg, M 1998 Monitoring proteolysis during cheddar cheese ripening using two-dimensional gel elctrophoresis. Journal of Food Science 63 423–428.CrossRefGoogle Scholar
D'Auria, E, Agostoni, C, Giovannini, M, Riva, E, Zetterstroem, R, Fortin, R, Greppi, F, Bonizzi, L, Roncada, P 2005 Proteomic evaluation of milk from different mammalian species as a substitute for breast milk. Acta Paediatrica 94 1708–1713CrossRefGoogle ScholarPubMed
Egito, AS, Miclo, L, López, C, Adam, A, Girardet, JM & Gaillard, JL 2002 Separation and characterization of mares’ milk αs1-, β-, κ-caseins, γ-casein-like, and proteose peptone component 5-like peptides. Journal of Dairy Science 85 697–706CrossRefGoogle ScholarPubMed
El-Agamy, EI 2006 Camel milk. In Hand Book of Milk of Non-Bovine Mammals (Ed. Park, Haenlan). USA: BlackwellGoogle Scholar
El-Agamy, EI 2007 The challenge of cow milk protein allergy. Small Ruminant Research 68 64–72CrossRefGoogle Scholar
El-Agamy, EI, Nawar, M, Shamsia, SM, Awad, S & Haenlein, GFW 2009 Are camel milk proteins convenient to the nutrition of cow milk allergic children. Small Ruminant Research 82 1–6CrossRefGoogle Scholar
Ereifej, KI, Alu'datt, H, AlKhalidy, HA, Alli, I & Rababah, T 2011 Comparison and characterisation of fat and protein composition for camel milk from eight Jordanian locations. Food Chemistry 127 282–289CrossRefGoogle Scholar
Farah, Z 1986 Effect of heat treatment on whey proteins of camel milk. Milchwissenschaft 41 763–765Google Scholar
Farah, Z & Farah-Riesen, M 1985 Separation and characterization of major components of camel milk casein. Milchwissenschaft 40 669–671Google Scholar
Fong, BY, Norris, CS & Palmano, KP 2008 Fractionation of bovine whey proteins and characterization by proteomic techniques. International Dairy Journal 18 23–46CrossRefGoogle Scholar
Ginger, MR & Grigor, MR 1999 Review Comparative aspects of milk caseins. Comparative Biochemistry and Physiology Part B 124 133–145CrossRefGoogle ScholarPubMed
Haenlein, GFW 2004 Goat milk in human nutrition. Small Ruminant Research 51 155–163CrossRefGoogle Scholar
Hinz, K, Huppertz, T, Kulozik, U & Kelly, AL 2007 Influence of enzymatic cross-linking on milk fat globules and emulsifying properties of milk proteins. International Dairy Journal 17 289–293CrossRefGoogle Scholar
Holland, JW, Deeth, HC & Alewood, PF 2004 Proteomic analysis of κ-casein micro-heterogeneity. Proteomics 4 743–752CrossRefGoogle ScholarPubMed
Holt, DL & Zeece, MG 1987 Two dimensional electrophoresis of bovine milk proteins. Journal of Dairy Science 71 2044–2050CrossRefGoogle Scholar
Kappeler, SR, Farah, Z & Puhan, Z 2003 5′-flanking regions of camel milk genes are highly similar to homologue regions of other species and can be divided into two distinct groups. Journal of Dairy Science 86 498–508CrossRefGoogle ScholarPubMed
Larsen, LB, Hinz, K, Jørgensen, ALW, Møller, HS, Wellnitz, O, Bruckmaier, RM, & Kelly, AL 2010 Proteomic and peptidomic study of proteolysis in quarter milk after infusion with lipoteichoic acid from Staphylococcus aureus. Journal of Dairy Science 93 5613–5626CrossRefGoogle ScholarPubMed
Lara-Villoslada, F, Olivares, M & Xaus, J 2005 The balance between caseins and whey proteins in cow's milk determines its allergenicity. Journal of Dairy Science 88 1654–1660CrossRefGoogle ScholarPubMed
Lindmark-Månsson, H, Timgren, A, Aldén, G & Paulsson, M 2004 Two-dimensional gel electrophoresis of proteins and peptides in bovine milk. International Dairy Journal 15 111–121CrossRefGoogle Scholar
Madkor, SA & Fox, PF 1991 Plasmin activity in buffalo milk. Food Chemistry 39 139–156CrossRefGoogle Scholar
Matéos, A, Girardet, JM, Molle, D, Dary, A, Miclo, L & Gaillard, JL 2009 Two-dimensional cartography of equine β-casein variants achieved by isolation of phosphorylation isoforms and control of the deamidation phenomenon. Journal of Dairy Science 92 2389–2399CrossRefGoogle ScholarPubMed
Merin, U, Bernstein, S, Bloch-Damti, A, Yagil, R, van Creveld, C, Lindner, P, Gollop, N 2001 A comparative study of milk serum proteins in camel and bovine colostrums. Livestock Production Science 67 297–301CrossRefGoogle Scholar
Pappa, EC, Robertsona, JA, Rigbya, NM, Mellona, F, Kandarakisb, I & Mills, ENC 2008 Application of proteomic techniques to protein and peptide profiling of Teléme cheese made from different types of milk. International Dairy Journal 18 605–614CrossRefGoogle Scholar
Park, YW 2001 Proteolysis and lipolysis of goats milk cheese. Journal of Dairy Science 84 84–92CrossRefGoogle Scholar
Rasmussen, LK, Johnsen, LB, Tsiora, A, Sørensen, ES, Thomsen, JK & Nielsen, NC 1999 Disulphide-linked caseins and casein micelles. International Dairy Journal 9 215–218CrossRefGoogle Scholar
Restani, P, Gaiaschi, A, Plebani, A, Beretta, B, Cavagni, G, Fiocchi, A, Poisei, C, Velona, T, Ugazio, AG & Galii, CL 1999 Crossreactivity between milk proteins from different animal species. Clinical and Experimental Allergy 29 997–1004CrossRefGoogle ScholarPubMed
Sanz Caballos, L, Morales, ER, de la Torre, Adarve G, Diaz, Castro J, Perez, Martinez L & Sanz, Sampelayo MR 2009 Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology. Journal of Food Composition and Analysis 22 322–329CrossRefGoogle Scholar
Santillo, A, Kelly, AL, Palermo, C, Sevi, A & Albenzio, M 2009 Role of indigenous enzymes in proteolysis of casein in caprine milk. International Dairy Journal 19 655–660CrossRefGoogle Scholar
Satija, KC, Rajpal, S, Pandey, R & Sharma, VK 1979 Electrophoresis of buffalo (Bos bubalis) serum proteins including immunoglobulins. Infection and Immunity 24 567–570CrossRefGoogle ScholarPubMed
Trujillo, AJ, Guamis, B & Carretero, C 1997 Hydrolysis of caprine β-casein by plasmin. Journal of Dairy Science 80 2258–2263CrossRefGoogle ScholarPubMed
West, DW 1986 Structure and function of the phosphorylated residues of casein. Journal of Dairy Research 53 333–352CrossRefGoogle ScholarPubMed
Work Group on Breastfeeding 1997 American Academy of Pediatrics: breastfeeding and the use of human milk. Pediatrics 100 1035–1039CrossRefGoogle Scholar