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Comparison of a simple butterfat agar medium with other media used for isolation and enumeration of lipolytic bacteria from dairy products

Published online by Cambridge University Press:  01 June 2009

Arthur W. Shelley
Affiliation:
Queensland Food Research Laboratories, Queensland Department of Primary Industries, Hamilton, Queensland 4007, Australia
Hilton C. Deeth
Affiliation:
Queensland Food Research Laboratories, Queensland Department of Primary Industries, Hamilton, Queensland 4007, Australia
Ian C. MacRae
Affiliation:
Department of Microbiology, University of Queensland, St Lucia, Queensland 4067, Australia

Summary

A nutrient agar medium containing 0·1 % of a low melting point fraction of butterfat was shown to be suitable for detection, enumeration and isolation of lipolytic bacteria from milk. Bacterial growth was not inhibited by the butterfat and lipolytic reactions were clearly visible and easily interpreted. Lipolytic counts on the butterfat agar compared favourably with lipolytic counts obtained with other commonly used media.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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