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Coagulase-negative staphylococci from non-mastitic bovine mammary gland: characterization of Staphylococcus chromogenes and Staphylococcus haemolyticus by antibiotic susceptibility testing and pulsed-field gel electrophoresis

Published online by Cambridge University Press:  09 November 2011

Mateja Pate ,
Irena Zdovc ,
Jana Avberšek ,
Matjaž Ocepek ,
Andrej Pengov  and
Ožbalt Podpečan
Mateja Pate*
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Irena Zdovc
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Jana Avberšek
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Matjaž Ocepek
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Andrej Pengov
Affiliation:
University of Ljubljana, Veterinary Faculty/National Veterinary Institute, Gerbičeva 60, 1000 Ljubljana, Slovenia
Ožbalt Podpečan
Affiliation:
Savinian Veterinary Policlinic, Celjska 3a, 3310 Žalec, Slovenia
*
*For correspondence; e-mail: [email protected]
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Abstract

During routine microbiological examination of milk samples from dairy cows without clinical signs of mastitis, quarter milk samples of 231 dairy cows from 12 herds were investigated for the presence of coagulase-negative staphylococci (CNS). The isolates were identified on the basis of colony morphology, Gram staining, catalase and coagulase test and the commercial kit, API Staph. CNS was detected in 29% (67/231) of the cows. A total of seven CNS species were identified with the most prevalent being Staphylococcus (Staph.) chromogenes (30%) and Staph. haemolyticus (28·8%), followed by Staph. simulans (11·2%), Staph. xylosus (11·2%), Staph. epidermidis (7·5%), Staph. hyicus (6·3%) and Staph. sciuri (5%). The predominant species, Staph. chromogenes and Staph. haemolyticus, were further characterized by antibiotic susceptibility testing using the agar disc diffusion method (Kirby-Bauer) and by pulsed-field gel electrophoresis (PFGE). Considerable resistance to ampicillin and penicillin was observed in both species. Isolates with identical or highly similar PFGE profiles were detected at the herd level despite a marked heterogeneity seen for both species. On the basis of somatic cell count, absence of clinical signs of inflammation and heterogeneity of genotypes, we assume that CNS isolated in this study could not be considered as important causative agents of the bovine mammary gland inflammation.

Keywords

Staphylococcus chromogenesStaphylococcus haemolyticusantibiotic susceptibility testingPFGEbovine milk
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 2 , May 2012 , pp. 129 - 134
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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