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Characterization of the 12% trichloroacetic acid-insoluble oligopeptides of Parmigiano-Reggiano cheese

Published online by Cambridge University Press:  01 June 2009

Francesco Addeo ,
Lina Chianese ,
Antonio Salzano ,
Raffaele Sacchi ,
Ugo Cappuccio ,
Pasquale Ferranti  and
Antonio Malorni
Francesco Addeo
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Lina Chianese
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Antonio Salzano
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Raffaele Sacchi
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Ugo Cappuccio
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Pasquale Ferranti
Affiliation:
Servizio di Spettrometria di Massa, CNR, via Pansini 5, 80131 Napoli, Italia and Istituto per la Chimica delle Molecole di Interesse Biologico, CNR, Arcofelice (NA), Italia
Antonio Malorni
Affiliation:
Servizio di Spettrometria di Massa, CNR, via Pansini 5, 80131 Napoli, Italia and Istituto per la Chimica delle Molecole di Interesse Biologico, CNR, Arcofelice (NA), Italia
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Summary

The isolation and identification of low molecular mass peptides formed during the ripening of Parmigiano-Reggiano cheese is described. A strategy was used based on the fractionation of nitrogenous material using chemical methods followed by HPLC to isolate peptides and fast atom bombardment-mass spectrometry to identify them. It was found that the majority of cheese oligopeptides arose from the proteolysis of β-casein. Several phosphopeptides and oligopeptides known in vivo to be biologically active have also been identified during the ripening of cheese.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 3 , August 1992 , pp. 401 - 411
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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References

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