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Characterisation of Staphylococcus aureus strains isolated from mastitis bovine milk in Argentina

Published online by Cambridge University Press:  22 February 2018

Mariela E Srednik ,
Valentine Usongo ,
Sarah Lépine ,
Xavier Janvier ,
Marie Archambault  and
Elida R Gentilini
Mariela E Srednik*
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Microbiología, Av. Chorroarín 280, C1427CWO, Buenos Aires, Argentina
Valentine Usongo
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Sarah Lépine
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Xavier Janvier
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Marie Archambault
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Elida R Gentilini
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Microbiología, Av. Chorroarín 280, C1427CWO, Buenos Aires, Argentina
*
*For correspondence; e-mail: [email protected]
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Abstract

The study reported in this Research Communication was conducted to characterise Staphylococcus aureus isolates recovered from mastitic bovine milk from dairy herds in Argentina. A total of 829 mastitic milk samples, both clinical and subclinical, were collected from 21 farms by veterinarians and submitted to the laboratory for testing from which 229 S. aureus isolates were recovered, an isolation rate of 28·1%. These isolates were tested for susceptibility to the antibiotics penicillin, erythromycin and clindamycin. Of the 229 isolates, 53 (23·1%) were resistant to penicillin, 31 (13·5%) to erythromycin and 28 (12·2%) to clindamycin. All isolates were negative for the mecA, mecC and pvl genes by PCR. Southernblot hybridisation revealed that the ermC gene was located on plasmid bands. Eighty isolates were randomly selected from the 229 for further characterisation. Restriction analysis of chromosomal DNA with Cf9I followed by PFGE of the 80 isolates revealed 23 distinct pulsotypes at 80% similarity. Seven major types (A, B, N, P, S, T, U and V) accounted for 68·7% of these isolates and 12 pulsotypes (A, B, F, G, J, K, M, N, P, S, T and U) occurred on more than one farm indicating genetic diversity within the farms. MLST of a representative isolate from dominant types identified the STs 97 705, 746, 2102 and 2187 with ST97 being the most predominant. Antibiotic susceptibility testing showed that 53·7% of the 80 randomly selected isolates were resistant to at least one of the three antibiotics tested. To our knowledge, this study represents the first large scale molecular studies on S. aureus isolates from dairy farms in Argentina.

Keywords

Staphylococcus aureusbovine mastitisantimicrobial resistancemolecular typing
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 1 , February 2018 , pp. 57 - 63
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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Footnotes

†

These authors contributed equally to the work.

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