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β-Casomorphin immunoreactive materials in cows' milk incubated with various bacterial species

Published online by Cambridge University Press:  01 June 2009

Ulrike Hamel ,
Gerhard Kielwein  and
Hansjörg Teschemacher
Ulrike Hamel
Affiliation:
Rudolf Buchheim-Institut for Pharmacology
Gerhard Kielwein
Affiliation:
Professor of Milk ·Hygiene and Technology, Justus Liebig-Universität Giessen, D-6300 Giessen, FRG
Hansjörg Teschemacher
Affiliation:
Rudolf Buchheim-Institut for Pharmacology
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Summary

To obtain information about the possible release of β-casomorphins from β-casein under in vitro conditions, cows' milk was incubated with 13 strains of Gram-negative or Gram-positive bacterial species isolated from bovine milk. After incubation periods of 1–24 d, milk samples were assayed for β-casomorphin-4, -5, -6 or -7 immunoreactive materials. In general, no β-casomorphin immunoreactive material was found in samples incubated with non-caseolytic strains, e.g. Pseudomonas putida or Streptococcus faecalis subsp. faecalis; however, high levels were found in several samples incubated with caseolytic strains, e.g. Ps. aeruginosa or Bacillus cereus. When such materials were present, the amounts of β-casomorphin-4, -6 or -7 greatly exceeded β-casomorphin-5 immunoreactivity. It was concluded that while some of these materials probably represented authentic β-casomorphins, others were more likely to be β-casomorphin precursors.

Type
Original Articles
Information
Journal of Dairy Research , Volume 52 , Issue 1 , February 1985 , pp. 139 - 148
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

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